Our laboratory investigates the biological chemistry and signaling pathways of nitric oxide by using biochemical and molecular methodologies in partnership with mass spectroscopy-based approaches. We uncovered that selective modification of cysteine residue by nitric oxide to generate S-nitrosocysteine regulates the function of enzymes participating in metabolism and mitochondrial bioenergetics. Current efforts are centered on uncovering the importance of S-nitrosocysteine in the regulation of B-oxidationof fatty acids and in the coordination of liver responses to metabolic deprivation or overloading. Another project investigates the utility of pharmacological agents that restore nitric oxide signaling to treat longchain fatty acid oxidation disorders.
Weare also generating inventories of cellular proteomes and secretomes of the mouse brain. These inventories are used to create 3D-functional landscapes of the mouse brain extracellular space and to identify astrocyte secreted proteins that influence neuronal development and neuronal demise. Other studies explore the role of nitric oxide and nitric-oxide mediated protein S-nitrosylation in the neuronastrocyte metabolism of glutamate.« Back to Previous Page