Methotrexate
- LIS Mnemonic: MTX
Collect
Gold (SST - Clot activator & gel)
Satellite locations must send Gold top tube
Volume Required
1 mL blood
Minimum Required
0.5 mL blood
Transport
Protect from light
Stability
4 hrs room temp, 24 hrs refrigerated
Processing
Place specimens in rack for 30 minutes prior to centrifugation to allow for complete clotting of sample. All chemistry specimens collected in a SST or PST should be centrifuged at 3,400 RPM's for 10 minutes. If there is gel separating the serum or plasma from the red cells, it is not necessary to transfer the serum or plasma to another tube. Follow directions in the centrifuge manual to set the centrifuge appropriately.If there is not gel separating the serum or plasma from the red cells, transfer the serum or plasma using a disposable pipette to another tube (pour off tube) being careful not to disturb the buffy coat. Rubber band the original tube (parent tube) with the labeled pour off tube prior to sending the specimen to CLS.
Days Performed
daily
Reported
Same day
Reflex Testing
N/A
CPT
83520
Methodology
TDX
Remarks
Daytime stat tat=3hrs. Red tube (serum) and light green tube (PST) light protected also acceptable (except CHOP satellites)
Contact Us
- (215) 590-1823
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