Immunogenetics Laboratory

HLA Class I Antibody Identification - Flow Cytometry

  • Synonyms: PRA, DSA (Post-Transplant)
  • LIS Mnemonic: HLAFID1

    Collect

    Red (No additive)

    Volume Required

    5ml

    Minimum Required

    4ml; Patients <20kg- 3ml

    Transport

    Room temperature

    Stability

    72 hrs

    Unacceptable conditions

    Samples older than 72 hours. Mislabeled samples: Samples must be labeled with at least two different identifiers, date & time of collection, and collector's signature.

Days Performed

Monday- Friday (7:30AM- 5:00PM)

Reported

5 days

Reflex Testing

N/A

CPT

86808

Methodology

Flow Cytometry Single Antigen Beads

Remarks

If patient has received transfusion(s) within two weeks prior to specimen collection, please notify the lab. It is recommended that antibody screening be performed again two weeks following any transfusion.

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