Immunogenetics Laboratory

HLA Typing - Low Resolution (HLA-A,B,C,DRB1,DRB3,4,5,DQB1)

  • Synonyms: HLA-A, HLA-B, HLA-C, HLA-DR, HLA-DQ

    Collect

    Lavender (EDTA), Cord Blood (1 ml), or 4 buccal brushes

    Volume Required

    4ml

    Minimum Required

    1ml (4ml if low WBC) or 4 buccal brushes

    Transport

    Room temperature

    Stability

    72 hrs

    Processing

    Buccal Brush Collection Instructions: Collect tissue by rolling the Buccal Brush firmly on the inside of the cheek, approximately 20 times on each side, making certain to move the brush over the entire cheek. 4 brushes should be collected per individual. Allow brushes to air dry for 10-15 minutes at room temperature. Return the dry brushes to their plastic sleeves and send to the laboratory.

    Unacceptable conditions

    Samples older than 72 hours. Mislabeled samples: Samples must be labeled with at least two different identifiers, date & time of collection, and collector's signature.

Days Performed

Monday- Friday (7:30AM- 5:00PM)

Reported

5 days

Reflex Testing

N/A

CPT

83890, 83898(x6), 83894(6), 88385 (x6)

Methodology

Sequence Specific Oligonucleotide Probes (SSOP) - Luminex

Remarks

Low Resolution HLA Typing includes HLA-A, B, C, DRB1,DRB3,4,5 and DQB1 loci.If patient has received multiple transfusion(s) within two weeks prior to collection, buccal cells are the recommended specimen type.

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