Shiga/E.coli toxin assay
- LIS Mnemonic: ECT
Collect
Stool or colonic aspirate in clean leak-proof container or Enteric Transport Medium (ETM)
Volume Required
1 tablespoon stool or 5mLs of colonic aspirate
Minimum Required
1 tablespoon stool or 5mLs of colonic aspirate
Transport
Unpreserved - immediately at room temperature
Stool in ETM - ASAP at room temperature
Stability
Specimens in ETM: 72 hours after collection
Specimens NOT in ETM: 1 hour after collection
Unacceptable conditions
Specimen submitted on a swab, containing barium or refrigerated
Days Performed
Monday - Friday, excluding holidays
Reported
Maximum of 72 hours after receipt in laboratory
CPT
87335
Methodology
EIA
Remarks
Recommended for any child with bloody diarrhea or a history of bloody diarrhea. If patient has had barium: specimen should not be submitted until >=7 days later.
The assay performed detects Shiga Toxins I and II.
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