Microbiology Laboratory

KOH Prep

  • LIS Mnemonic: KOH

    Collect

    Skin, nails or hair in sterile container

    Volume Required

    5-6 Flakes, 5-6 Hairs, 1 Nail Clipping

    Minimum Required

    5-6 Flakes, 5-6 Hairs, 1 Nail Clipping

    Transport

    Room temperature

    Stability

    Up to 72 hours after collection

    Unacceptable conditions

    Specimens submitted on swabs are not acceptable since the cotton strands can resemble hyphae.

Days Performed

Daily

Reported

Same day

CPT

87210

Methodology

Direct Examination

Remarks

This test is only performed on skin, hair and nails. Skin areas should be well disinfected with alcohol. Scraping should be taken from the leading edge of the lesion. Nails should be scraped with a sterile scalpel blade.

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