Microbiology Laboratory

Tissue Culture

  • LIS Mnemonic: CWD

    Collect

    Tissue specimen in a sterile leak-proof screw-capped container-(For Surgical specimens, see Surgical Specimen Collection Algorithm)

    Volume Required

    A larger specimen yields better recovery of pathogens

    Minimum Required

    Tiny pieces are discouraged due to decreased yield

    Transport

    Send To Lab Immediately at room temperature. Submit in sterile container wrapped in moist sterile guaze to prevent drying.

    Stability

    Up to 1 hour if tissue kept moist

    Unacceptable conditions

    Tissue in Formalin; dried out tissue

Days Performed

Daily

Reported

Daily updates until final

CPT

87070

Methodology

Culture

Remarks

Avoid contamination from sinus tracts and/or skin when collecting specimen. Do not insert tissue specimen into a swab set as this complicates aseptic specimen processing in the laboratory.

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