Microbiology Laboratory

Bordetella Pertussis PCR

  • Synonyms: Whooping Cough
  • LIS Mnemonic: BPPCR

    Collect

    Nasal aspirate in a leukens trap or sterile leak-proof screw-cap leak proof container

    Volume Required

    1 - 5mLs

    Minimum Required

    0.5mLs

    Transport

    Send To Lab Immediately or if transport is delayed, freeze and send on ice.Must be received by 7am for same-day result.Do not send in Viral Transport Media.

    Stability

    Specimen must be frozen and sent on ice.

    Unacceptable conditions

    Specimen not frozen or sent on ice.

Days Performed

Weekdays (excluding holidays)

Reported

same day - generally by 1pm if received by 7am

CPT

83898, 83890, 83892, 83894, 83896, 83912

Methodology

PCR

Remarks

PCR is the methodology of choice for diagnosis;It is critical to adhere to transport instructions as enzymes in the specimen will degrade nucleic acid over time and promote false negative results. PCR detects nucleic acid, not viable organisms, so it should not be used as a test of cure. Duplicate specimens are not necessary

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