Epstein-Barr Virus (EBV) PCR (Qualitative) - CSF specimen
- LIS Mnemonic: EBVPCR
Collect
CSF
Volume Required
1 ml
Minimum Required
0.5 ml
Transport
Keep specimen at 4C
Processing
If an extended delay in transport of specimens is anticipated, rapidly freeze the specimens to at least -60°C and transport to the laboratory on dry ice. Please consult the laboratory if necessary.
Days Performed
Daily
Reported
Same day
Reflex Testing
N/A
CPT
87798
Methodology
Amplification and detection of EBV DNA of the nonglycosylated membrane protein BNRF1 p143 gene using TaqMan real-time PCR technology. This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.
Interpretation
If positive, results are reported as Epstein-Barr virus DNA detected.
Reference Values
Negative or no Epstein-Barr virus DNA detected
Remarks
Clinical Utility: EBV is the primary cause of infectious mononucleosis with symptoms of fever, exudative pharyngitis, lymphadenopathy, enlarged liver and spleen, and an atypical lymphocytosis. Infections with EBV can lead to hepatitis, pneumonia, myocarditis, neurologic syndromes, hemolytic anemia, thrombocytopenia, and hemophagocyctic syndrome. The virus is also associated with posttransplantation lymphoproliferative disorders (PTLD), Burkitt lymphoma, X-linked lymphoproliferative syndrome, nasopharyngeal carcinoma, and lymphomas of the CNS. The diagnosis of EBV infection is increasingly being made by PCR, and EBV DNA has been detected in specimens from solid-organ and bone marrow transplant recipients and HIV-infected patients with lymphoproliferative disorders; individuals with infectious mononucleosis, and in patients with other manifestations of primary or recurrent EBV infections. Quantitative PCR assays should be used in immunocompromised patients to associate infection with disease, predict and monitor disease progression, assess efficacy of antiviral therapy, and to facilitate our understanding of the pathogenesis of EBV. In these patients, viremia is considered to be the best predictor of disease, and quantitative measures of EBV DNA in blood is useful for the continued surveillance and management of transplant patients.
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