BK Virus PCR (Qualitative) - urine specimen
- LIS Mnemonic: BKVPCR
Collect
Random Urine Container
Volume Required
5-10 ml of clean voided urine
Minimum Required
5ml of clean voided urine
Transport
Keep specimen at 4C
Processing
For infants, a volume of 1 to 2 ml of urine is acceptable. If an extended delay in transport of specimens is anticipated, rapidly freeze the specimens to at least -60°C and transport to the laboratory on dry ice. Please consult the laboratory if necessary.
Days Performed
Daily
Reported
Same day
Reflex Testing
Specimens that are positive for BK virus DNA by the qualitative real-time PCR will recieve a quantitative BK virus PCR to determine the copies/ml of BKV DNA within the clinical material.
CPT
87798 (qualitative BK virus PCR); 87799 (quantitative BK virus PCR)
Methodology
Amplification and detection of BK virus DNA using real-time TaqMan PCR and nucleic acid primer/probe pairs specific for conserved regions of the BK virus genome. This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.
Interpretation
If positive, results are reported as BK virus DNA detected.
Reference Values
Negative or no BK virus DNA detected
Remarks
Clinical Utility: BK virus can cause ureteral ulcerations, stenosis, and hemorrhagic cystitis in renal transplant recipients. Persistent active replication of BKV in renal transplant recipients also has been associated with progressive loss of the graft due to BKV-associated nephropathy. BKV-associated hemorrhagic cystitis is a major complication of bone marrow transplants. Continuous monitoring of urine and plasma by real-time PCR is desirable to predict disease and follow recovery over time.
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