HLA Typing - Confirmatory (HLA-A,B,DRB1)
- Synonyms: HLA-A,B,DR
- LIS Mnemonic: HLA CONFIRM
Collect
Lavender (EDTA), Cord Blood (1 ml), or 4 buccal brushes
Volume Required
4ml
Minimum Required
1ml (4ml if low WBC) or 4 buccal brushes
Transport
Room temperature
Stability
72 hrs
Processing
Buccal Brush Collection Instructions: Collect tissue by rolling the Buccal Brush firmly on the inside of the cheek, approximately 20 times on each side, making certain to move the brush over the entire cheek. 4 brushes should be collected per individual. Allow brushes to air dry for 10-15 minutes at room temperature. Return the dry brushes to their plastic sleeves and send to the laboratory.
Unacceptable conditions
Samples older than 72 hours. Mislabeled samples: Samples must be labeled with at least two different identifiers, date & time of collection, and collector's signature.
Days Performed
Monday- Friday (7:30AM- 5:00PM)
Reported
5 days
Reflex Testing
N/A
CPT
83890, 83898(x3), 83894(3), 88385 (x3)
Methodology
Sequence Specific Oligonucleotide Probes (SSOP) – Luminex
Remarks
This test is for confirmatory typing of stem cell transplant patients and donors that have already been typed at high resolution with a different sample. Confirmatory HLA Typing includes HLA-A, B, and DRB1 loci. If patient has received multiple transfusion(s) within two weeks prior to collection, buccal cells are the recommended specimen type.
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- (215) 590-5648
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