Frozen tumor tissue
0.5 x 0.5 x 0.5 cm tumor tissue
0.5 x 0.5 x 0.2 cm tumor tissue
Monday - Friday
Mutation analysis of BRAF gene, detection and identification of KIAA1549-BRAF fusion products
1. RT-PCR for KIAA1549-BRAF fusion products is performed;
2. If no fusion products are detected, PCR amplification and direct sequencing of BRAF exons 11 and 15 are performed on the DNA;
3. If no BRAF mutations are detected a whole genome SNP array analysis of the DNA is performed.