Immunogenetics Laboratory

Flow Cytometry T and B Cell Crossmatch

  • Synonyms: Flow Cytometry Crossmatch, HLA Crossmatch
  • LIS Mnemonic: HLAFXM

    Collect

    Recipient: Red top and Yellow tops (ACD); Donor: Yellow tops

    Volume Required

    Recipient: 7ml (Red top) and 20-30ml (ACD); Donor: 20-25ml (ACD)

    Minimum Required

    3ml (Red top) and 10ml (ACD); Donor: 20-25ml (ACD)

    Transport

    Room temperature

    Stability

    24 hrs

    Unacceptable conditions

    Samples older than 24 hours. Samples must be labeled with at least two different identifiers, date & time of collection, and collector's signature.

Days Performed

Monday- Friday (7:30AM- 5:00PM)

Reported

1-2 days; 4-5 hours for Deceased Donor Crossmatch

Reflex Testing

N/A

CPT

86805 x 2

Methodology

Flow Cytometry (T and B Cells)

Remarks

If the patient has received transfusion(s) within two weeks prior to specimen collection, please notify the lab. It is recommended that antibody screening and crossmatching be performed again two weeks following any transfusion.

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