Confirmatory Low Resolution HLA-A,B,DRB1 Typing
- Synonyms: Confirmatory HLA-A,B,DR (EPIC)
- LIS Mnemonic: HLACONFIRM
Collect
Lavender (EDTA), Cord Blood (1 ml), or 4 buccal brushes
Volume Required
4ml
Minimum Required
1ml (4ml if low WBC) or 4 buccal brushes
Transport
Room temperature
Stability
72 hrs
Processing
Buccal Brush Collection Instructions: Collect tissue by rolling the Buccal Brush firmly on the inside of the cheek, approximately 20 times on each side, making certain to move the brush over the entire cheek. 4 brushes should be collected per individual. Allow brushes to air dry for 10-15 minutes at room temperature. Return the dry brushes to their plastic sleeves and send to the laboratory.
Unacceptable conditions
Samples older than 72 hours. Mislabeled samples: Samples must be labeled with at least two different identifiers, date & time of collection, and collector's signature.
Days Performed
Monday- Friday (7:30AM- 5:00PM)
Reported
5 days
Reflex Testing
N/A
CPT
81371
Methodology
Sequence Specific Oligonucleotide Probes (SSOP)
Remarks
This test is for confirmatory typing of stem cell transplant patients and donors that have already been typed at high resolution with a different sample. Confirmatory HLA Typing includes HLA-A, B, and DRB1 loci. If patient has received multiple transfusion(s) within two weeks prior to collection, buccal cells are the recommended specimen type.
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- (215) 590-5648
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