Bone marrow in EDTA anticoagulated tube
Keep specimen at 4C
Amplification and detection of adenovirus DNA using real-time TaqMan PCR and nucleic acid primer/probe pairs specific for conserved regions of the adenovirus genome. Detects all 51 known serotypes of adenoviruses; does not differentiate by type. This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.
If positive, results are reported as adenovirus DNA detected.
Negative or no adenovirus DNA detected
Clinical Utility: RESPIRATORY: Adenoviruses are important causes of acute upper and lower respiratory tract illnesses in immunocompetent infants and children. Manifestations include the common cold, pharyngitis, tonsillitis, conjunctivitis, pharyngoconjuntival fever, croup, a pertussis-like syndrome, bronchiolitis, and pneumonia. OCULAR: Adenoviruses are commonly associated with acute follicular conjunctivitis and pharyngoconjunctival fever, and less frequently with epidemic keratoconjunctivitis (occurs mainly in adults) in immunocompetent individuals. GASTROINTESTINAL: The fastidious adenovirus types 40 and 41 can cause viral gastroenteritis. These adenovirus types are widespread and cause endemic diarrhea and outbreaks of diarrhea; infections appear to occur year-round. DISSEMINATED INFECTIONS: Adenoviruses can cause life-threatening disseminated infections in young infants and immunocompromised hosts such as bone marrow, liver, kidney, and lung transplant recipients and patients with primary immunodeficiency disorders, AIDS or malignancies. Clinical manifestations of adenovirus disease in immunocompromised patients include pneumonia, hepatitis, nephritis, colitis, encephalitis, hemorrhagic cystitis, and myocarditis. Detection of adenovirus DNA in blood plasma is useful for identifying immunocompromised patients at risk for invasive disease. Depending on the particular patient and specific disease, other specimens may be submitted as well. These include bone marrow, tissue (e.g., liver, lung, kidney, colon, endomyocardial biopsy), CSF, urine, respiratory, conjunctival, and stool. PCR of bone marrow can be helpful in patients suspected of having adenovirus-associated bone marrow suppression or hemophagocytic syndrome.
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