Stool in a sterile, leak-proof container
2 to 4 ml of liquid stool; 2 to 4 grams of formed stool
2 ml of liquid stool; 2 grams of formed stool
Keep specimen at 4C
The specimen of choice is stool. Rectal swabs are inferior to stool specimens for the detection of viruses and are discouraged. If it is necessary to collect a rectal swab, a sufficient quantity of fecal material (at least a pea-sized amount) should be obtained. If fecal material is not clearly visible on the swab, the specimen is most likely inadequate. Rectal swabs should be immediately placed in viral transport medium.
Swab specimens not received in viral transport medium or received in bacteriological transport medium are discouraged. DO NOT USE CALCIUM ALGINATE OR WOODEN SHAFT SWABS FOR COLLECTION OF SPECIMENS; ONLY USE DACRON OR RAYON TIPPED SWABS ON PLASTIC OR METAL SHAFTS.
Amplification and detection of HSV DNA polymerase gene using TaqMan real-time PCR technology. This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.
If positive, results are reported as herpes simplex virus DNA detected.
Negative or no herpes simplex virus DNA detected
Clinical Utility: PCR of CSF specimens is now considered the 'gold standard' for the detection of HSV in patients with HSV encephalitis or other HSV-related central nervous system diseases such as recurrent meningitis. PCR can also be used to accurately diagnose HSV infections of the skin and mucous membranes and for the diagnosis of HSV neonatal disease. For diagnosis of neonatal HSV infection, collect specimens from skin vesicles, conjunctiva, mouth or nasopharynx, urine, blood, stool or rectum, and CSF. Detection of HSV from any of these sites more than 48 hours after birth may indicate active viral replication and infection of the infant rather than colonization after intrapartum exposure.
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