Single-cell Analysis of Embryonic Hematopoiesis and Tumor Microenvironment

Emerging single-cell and in situ technologies are now facilitating the multi-omic characterization of all major cell types and their interactions in a complex tissue, revolutionizing multiple disciplines such as developmental biology and cancer biology. In ongoing projects, we are using single-cell RNA-Seq to identify novel precursor cell populations of embryonic HSCs. Additionally, as a member of the NCI-funded Human Tumor Atlas Network, we are leading the Center for Pediatric Tumor Cell Atlas to better understand clonal evolution and tumor heterogeneity at single-cell level for three types of high-risk pediatric cancers.