Erin H. Graf, PhD, D(ABMM)

Erin H. Graf, PhD, D(ABMM)

Erin H. Graf, PhD, D(ABMM), is Director of the Infectious Disease Diagnostics Lab at Children's Hospital of Philadelphia.

Locations: Main Campus
Appointments and Referrals: 1-800-TRY-CHOP (1-800-879-2467)

Education and Training

Fellowship

Medical Microbiology and Infectious Disease Serology - University of Utah/ARUP Laboratories, Salt Lake City, UT

Additional Training

CDC/APHL Emerging Infectious Diseases Advanced Laboratory Training - University of Iowa Hygienic Laboratory, Iowa City, IA

Board Certification

Medical Microbiology

Undergraduate Degree

BS in Cell Biology and Molecular Genetics - University of Maryland, College Park, MD

Graduate Degree

PhD in Cell and Molecular Biology - University of Pennsylvania, Philadelphia, PA

Titles and Academic Titles

Director, Infectious Disease Diagnostics Lab

Assistant Professor of Clinical Pathology and Laboratory Medicine, Perelman School of Medicine at the University of Pennsylvania

Departments and Services

Publications

Papers

2017

Sandra Montgomery, Kiana Roman, Lan M. Nguyen, Ana Maria Cardenas, James J. Knox, Erin H. Graf: Prospective evaluation of light scatter technology paired with MALDI-TOF mass spectrometry for rapid diagnosis of urinary tract infections. Journal of Clinical Microbiology 55(6): 1802-1811, May 2017.

2016

Danielle Fortuna, Ana M Cárdenas, Erin HGraf, Larry A Harshyne, D.Craig Hooper, John Shields,Mark Thomas Curtis: Human parechovirus and enterovirus initiate distinct CNS innateimmune responses: pathogenic and diagnostic implications. Journal of Clinical Virology 86: 39-45, November 2016.

Graf EH, Farquharson MV, Cardenas AM.: Comparative Evaluation of the FilmArray Meningitis/Encephalitis Molecular Panel in a Pediatric Population. Diagnostic Microbiology and Infectious Disease Accepted(pii: S0732-8893(16)30321-2), October 2016.

Pinzone M,Graf E, Lynch L, McLaughlin B, Hecht F, Migueles S, Nunnari G, O'Doherty U: Monitoring integration over time supports a role for CTL and ongoing replication as determinants of reservoir size. Journal of Virology pii: JVI.00242-16, September 2016.

Flygare S, Simmon KE, Miller C, Qiao Y, Kennedy B, DiSera T, Graf EH, Tardif KD, Kapusta A, Rynearson S, Stockman C, Queen K, Tong S, Volkerding K, Blaschke, Byington C, Jain S, Pavia A, Ampofo K, Eilbeck K, Marth G, Yandell M, Schlaberg R. : Ineractive web-based metagenomics analysis portal for universal pathogen detection and host response-based diagnosis and discovery. Genome Biology 17(1): 111, May 2016.

Erin H. Graf, Keith E. Simmon, Keith D. Tardif, Weston Hymas, Steven Flygare, Karen Eilbeck, Mark Yandell and Robert Schlaberg: Unbiased Detection of Respiratory Viruses Using RNA-seq-Based Metagenomics: A Systematic Comparison to A Commercial PCR Panel. Journal of Clinical Microbiology 54(4): 1000-1007, March 2016.

2013

Pace MJ*, Graf EH* and O'Doherty U: HIV 2-long terminal repeat circular DNA is stable in primary CD4+T cells Virology 6822((7)): 143-8, 2013.

Hatano H, Yukl SA, Ferre AL, Graf EH, Somsouk M, Sinclair E, Abdel-Mohsen M, Liegler T, Harvill K, Hoh R, Palmer S, Bacchetti P, Hunt PW, Martin JN, McCune JM, Tracy RP, Busch MP, O'Doherty U, Schacklett BL, Wong JK, Deeks SG : Prospective Antiretroviral Treatment of Asymptomatic, HIV-1 Infected Controllers. PLoS Pathogens 9((10)): e1003691, 2013.

Graf EH, Pace MJ, Peterson BA, Lynch LJ, Chukwulebe S, Mexas AM, Shaheen F, Martin JN, Deeks SG, Connors M, Migueles SA and O'Doherty U : Gag-positive reservoir cells are susceptible to HIV-specific cytotoxic-T lymphocyte mediated clearance in vitro and can be detected in vivo. PLoS One 8((8)): e71879, 2013.

Eriksson S*, Graf EH*, Dahl V*, Strain MC*, Yukl S*, Lysenko ES, BoschRJ, Lai J, Chioma S, Emad F, Abdel-Mohsen M, Hoh R, Hecht F, Hunt P, Somosouk M, Wong JK, Siliciano RF, Richman D, O'Doherty U, Palmer S, Deeks SG, Siliciano JD : Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies. PLoS Pathogens. PLoS Pathogens 9((2)): e1003174, 2013.

2012

Pace MJ, Graf EH, Agosto LM, Mexas AM, Male F, Brady T, Bushman FD, O'Doherty: Directly infected resting CD4+T cells can produce HIV Gag without spreading infection in a model of HIV latency. PLoS Pathogens 8((7)): e1002818, 2012.

2011

Pace MJ, Agosto LM, Graf EH, O'Doherty U : HIV reservoirs and latency models. Virology 411(2): 344-54, 2011.

Mendoza D, Johnson SA, Peterson BA, Natarajan V, Dewar RL, Burbelo PD, Doria- Rose NA, Graf EH, Greenwald JH, Hodge JN, Thompson WL Cogliano NA, Chairez CL, Rehm CA, Jones S, Hallahan CW, Kovacs JA, Sereti I, Sued O, O'Connell RJ, O'Doherty U, Chun TW, Connors M and Migueles SA : Comprehensive Analysis of Unique Cases with Profound Control over HIV Replication. Blood 119(20): 4645-55, 2011.

Graf EH, Mexas AM, Yu JJ, Shaheen F, Liszewski MK, Di Mascio M, Migueles SA, Connors M, O'Doherty U: Elite suppressors harbor low levels of integrated HIV DNA and high levels of 2-LTR circular HIV DNA compared to HIV+ patients on and off HAART PLoS Pathogens 7((2)): e1001300, 2011.

Agosto LM, Liszewski MK, Mexas A, Graf E, Pace M, Yu JJ, Bhandoola A, O'Doherty U: Patients on HAART often have an excess of unintegrated HIV DNA: implications for monitoring reservoirs. Virology 409(1): 46-53, 2011.

Abstracts

2017

Natasha Lopez Fisher, Stephanie Mitchell, Caitlin Dougherty, Bryan Krock and Erin Graf: Cell-free DNA for pathogen detection in the diagnosis of invasive infections. ASM Conference on Rapid Applied Microbial Next-Generation Sequencing and Bioinformatic Pipelines 2017.

Books

Chapters

2016

Erin H. Graf: Chlamydia trachomatis, Neisseria gonorrhoeae. Clinical Microbiology Procedures Handbook, 4th Edition. Amy Leber (eds.). 12(3), July 2016.

Posters and Presentations

2017

Julia S. Sammons, Sara Townsend, Cindy L. Hoegg, Sarah A. Smathers, Erin H. Graf, Susan Coffin, Katie Williams, Eileen Farnon, Ursula Nawab, David Munson, Kevin Dysart, Gil Binenbaum: Outbreak of Adenovirus in a Neonatal Intensive Care Unit: Rapid Detection and Successful Prevention of Secondary Transmission. Oral presentation at the Association for Professionals in Infection Control Annual Meeting June 2017.

Stephanie Mitchell and Erin H. Graf: Limb Weakness in an 8 year old. Oral case presentation at the Clinical Virology Symposium May 2017.

Reddy V Ponaka, Clarissa Gomez, Denise Patel, Vladimir Slepnev, José R. Romero, Erin Graf, Slava Elagin: illumigene Kingella by Meridian Bioscience: Isothermal Amplification test for the Detection of Kingella kingae from Synovial Fluids. Poster presentation for the Clinical Virology Symposium May 2017.

Mitchell SL, Sammons JS, Graf EH: Real-time Molecular Serotyping and Improved Resolution Through Whole-Virus Genome Sequencing During an Adenovirus Outbreak in a Neonatal Intensive Care Unit. Poster presentation for Clinical Virology Symposium May 2017 Notes: **Recipient of the "Edith Hsuing Travel Award" for outstanding abstract.

Laurel Glaser, Julia Was, Erin Graf: Multi-locus Amplification Followed by Next Generation Sequencing and Metagenomic Analysis Dramatically Improves Diagnostic Yield Over Sanger-Based Pan-Bacterial Sequencing in Formalin Fixed Paraffin Embedded Tissues Poster presentation at ASM Microbe May 2017.

Erlinda Ulloa, Erin Graf, Ana Maria Cardenas, Michael Silverman, Eimear Kitt: Malaria Trojan Horse: A case of mixed-species malaria infection Poster presentation at European Society for Pediatric Infectious Diseases Annual Meeting May 2017.

E. Kitt, E.H. Graf, J.M. Bergelson: Dengue Associated Hemophagocytic Lymphohistiocytosis. Poster presentation at European Society for Pediatric Infectious Diseases Annual Meeting May 2017.

Robert Schlaberg, Heng Xie, Steven Flygare, Yuying Mei, Hajime Matsuzaki, Mark Yandell, Erin Graf: Detection of Previously Missed Pathogens in Immunocompromised Children with Suspected Pulmonary Infections by a Fully-Validated Next-Generation Sequencing Test Poster Presentation at the American Thoracic Society Annual Meeting May 2017.

Invited Lectures

2017

Graf E. "Data Analysis", Molecular Virology Workshop, Savannah, GA. May 2017.

Graf E. "From base pairs to bedside: practical applications of sequencing technologies in the clinical microbiology laboratory", Pennsylvania American Society for Clinical Laboratory Science, King of Prussia, PA. May 2017.

Awards and Honors

2014, First Place for Fellows' Research Presentations, University of Utah School of Medicine, Department of Pathology

2013, University of Utah School of Medicine, Department of Pathology Research Grant Award

2012, Pan American Society for Clinical Virology Travel Award

2012-2013, HIV Pathogenesis T32 predoctoral training grant recipient

2012, Young Investigator Award, 19th annual Conference on Retroviruses and Opportunistic Infections, Seattle, WA

2012, President Gutmann Leadership Award, University of Pennsylvania

2011, Young Investigator Award, 18th annual Conference on Retroviruses and Opportunistic Infections, Boston, MA

2008-2013, Howard Huges Medical Institute Med-into-Grad Scholar

Editorial and Academic Positions

Academic and Institutional Committees

2017, Intraoperative Lab Orders, Assessment Committee Member

2016-2017, HIV Policy revision, committee member

2016-present, Meningitis Pathway, Committee Member

2016, Increasing Value in Respiratory Virus Testing, Temporary Committee Member

2015-present, Antibiotic Drug Use Evaluation, Committee Member

2015-present, Influenza Pathway, Committee Member

Leadership and Memberships

Memberships in Professional Organizations

2014-present, Association for Molecular Pathology

2011-present, American Society for Microbiology

 - 2017-present, Development Committee Member