Manuela Lavorato, PhD Manuela Lavorato, PhD, is a research associate with Mitochondrial Medicine and the Division of Human Genetics at Children’s Hospital of Philadelphia. Appointments and Referrals: 1-800-TRY-CHOP (1-800-879-2467) Education and Training FellowshipPostdoctoral Fellow - Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA Undergraduate DegreeBachelor’s Degree in Biology - University of Calabria, Italty Graduate DegreePhD in Animal Biology - University of Calabria, Italy Master’s Degree in Biology (molecular pathology) - University of Calabria, Italy Titles and Academic Titles Researcher Departments and Services Division of Human GeneticsMitochondrial Medicine Research Interests Electron microscopy and live-cell imaging Excitation contraction coupling Skeletal and cardiac muscle diseases Mitochondrial diseases Mitochondria morphology and dynamics Ecotoxicology Video-tracking analysis of amphibians affected by neurotoxic pesticides impairing neuromuscular system Publications Papers2017 Lavorato M, Iyer V R, Dewight W, Cupo R R, Debattisti V, Gomez L, De la Fuente S, Zhao Y-T, Valdivia H H, Hajnóczky G, Franzini-Armstrong C. Increased mitochondrial nanotunneling activity, induced by calcium imbalance, affects intermitochondrial matrix exchanges. Proc Natl Acad Sci U S A. 2017 Jan 31;114(5):E849-E858. doi: 10.1073/pnas.1617788113. Epub 2017 Jan 17. Perni S, Lavorato M, Beam KG. De novo reconstitution reveals the proteins required for skeletal muscle voltage-induced Ca2+ release. Proc Natl Acad Sci USA 114 (52), 13822-13827. Proc Natl Acad Sci U S A. 2017 Dec 26;114(52):13822-13827. doi: 10.1073/pnas.1716461115. Epub 2017 Dec 11. 2016 Lavorato M, Gupta P K, Hopkins P M, Franzini-Armstrong C.Skeletal muscle microalterations in patients carrying Malignant Hyperthermia-related mutations of the e-c coupling machinery. Eur J Transl Myol. 2016 Sep 15;26(4):6105. doi: 10.4081/ejtm.2016.6105. eCollection 2016 Sep 15. Linsley J W, Hsu I-U, Groomc L, Yarotskyy V, Lavorato M, Horstick E J , Linsley D, Wang W, Franzini-Armstrong C, Dirksen R T, Kuwada J Y, 2016. Congenital myopathy results from misregulation of a muscle Ca2+ channel by mutant Stac3. Proc Natl Acad Sci USA, 114(2): E228–E236. 2015 Lavorato M, Taiqin H, Iyer V R, Perni S, Meissner G, and Franzini-Armstrong C, 2015. Dyad content is reduced in cardiac myocytes of mice with impaired calmodulin regulation of RyR2. J Muscle Res Cell Motil. 36(2):205-14. 2013 Lavorato M, Bernabò I, Crescente A, Denoël M, Tripepi, Brunelli E, 2013. Endosulfan Effects on Rana dalmatina Tadpoles: Quantitative Developmental and Behavioural Analysis. Arch Environ Contam Toxicol. 2013 Feb;64(2):253-62. doi: 10.1007/s00244-012-9819-7. Epub 2012 Oct 13. Abstracts2017 Lavorato M. Mitochondria fission in fatigued muscle. Gordon Research Seminar and Gordon Conference, Muscle: Excitation-Contraction Coupling, 2017. Les Diablerets, Switzerland. Lavorato M, Iyer V. R, Franzini-Armstrong C. A convenient alternative view of muscle membranes. Gordon Conference, Muscle: Excitation-Contraction Coupling, 2017. Les Diablerets, Switzerland. Madan A, Viswanathan M C, Lavorato M, Schmidt W, Ochala J, Cammarato A, 2017. ACTA1-Related Nemaline Myopathy Mutations Engender a Range of Structural and Functional Phenotypes in Drosophila Indirect Flight Muscles. Biophys J, 112 (3) p182a. 2016 Iyer V R, Monfredi O, Maltsev V, Lavorato M; Stern M, Franzini-Armstrong C, 2016. SR Contribution to Calcium Cycling in Sino-Atrial Node Cells: As seen from Nanoscale Electron Microscopy and Numerical Modeling. Biophys J 110, 359a. Lavorato M, Ramesh V I, Zhao Y-T, Valdivia H H, Franzini-Armstrong C, 2016. Electron tomography of mitochondrial nanotunnels in a CPVT model with RyR2 loss-of-function mutation. Biophys J 110, 367a. 2015 Lavorato M, Schmidt W, Viswanathan M C, Ochala J, Franzini-Armstrong C, Cammarato A, 2015. The actin F352S nemaline myopathy mutation disrupts indirect flight muscle structure and function in Drosophila. Biophys J 110, 128a. Iyer V R, Lavorato M, Zhao Y-T, Valdivia H H, Franzini-Armstrong C, 2015. Mitochondrial abnormalities in a mouse CPVT model with RyR2 loss-of-function mutation. Biophys J 108, 132a. Beqollari D, Romberg CF, Feng W, Lopez JR, Lavorato M, Perni S, Hopkins PM, Franzini-Armstrong C, Pessah IN, Allen PD, Beam KG, Bannister RA, 2015. Calcium channel dysfunction in a mutant mouse model of malignant hyperthermia (CaV1.1 R174W). Biophys J 108, 504a. Iyer V R*, Lavorato M*, Zhao YT, Valdivia H., Franzini-Armstrong C. Ultrastructural cardiac alterations resulting from cardiac RyR2 mutations with opposite functions. * contributed equally. Gordon Conference: Muscle: Excitation-Contraction Coupling, 2015, Sunday River in Newry ME United States. Posters and Presentations2017 Lavorato M. Gordon Research Conference and Gordon Research Seminar. Muscle: Excitation -Contraction Coupling. Les Diablerets, Switzerland. Selected talk, two posters’ presenter. 2016 Lavorato M. 60th Biophysical Society Annual Meeting. Selected talk and poster presenter. Lavorato M. Biomedical Postdoctoral research symposium, University of Pennsylvania, Philadelphia, PA. Poster presenter. 2015 Lavorato M. Gordon Conference: Muscle: Excitation-Contraction Coupling, 2015, Sunday River in Newry ME United States. Selected talk and poster presenter. Lavorato M. 59th Biophysical Society Annual Meeting, Baltimore, Maryland (poster presenter). Lavorato M. Biomedical Postdoctoral research symposium. Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA. Poster presenter. Invited Lectures 2017 Lavorato M. Mitochondrial nanotunnels in a CPVT model with RyR2 loss-of-function mutation. The ISHR-Latin American Section-Sponsored Symposia during the XXIV Congreso Argentino de Hipertensión, Mendoza, Argentina. 2015 Lavorato M. Invited speaker: Strange mitochondrial behavior. Friendly or not? At the Mitocare, Jefferson University, Philadelphia PA Awards and Honors 2017, FEBS Journal Poster Prize at the GRC on Muscle: Excitation-Contraction Coupling, Les Diablerets, Switzerland 2010-2011, Two International Mobility Fellowships for PhD students, Department of Environmental Sciences, Behavioral Biology Unit, University of Liège (Belgium) 2009, Bonino Pulejo Foundation Fellowship, Messina, Italy Leadership and Memberships Memberships in Professional Organizations 2012-present, Biophysical Society Patient Experience Ratings About the Patient Experience Rating System The Patient Experience Rating is an average of all responses to the care provider related questions shown below from our nationally-recognized Press Ganey Patient Satisfaction Survey. Patients that are treated in outpatient or hospital environments may receive different surveys, and the volume of responses will vary by question. Responses are measured on a scale of 1 to 5 with 5 being the best score. 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