Itzhak Nissim, PhD

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Itzhak Nissim, PhD, is a researcher with the Division of Human Genetics and the Director of the Metabolomics Core at Children's Hospital of Philadelphia.

Locations: Main Campus
Appointments and Referrals: 1-800-TRY-CHOP (1-800-879-2467)

Education and Training

Graduate Degree

MSc in Life Science - Hebrew University, Israel
PhD in Biochemistry & Biophysics - Weizmann Institute, Israel

Titles and Academic Titles

Director, Metabolomics Core

Researcher

Professor of Pediatrics (Primary), Perelman School of Medicine at the University of Pennsylvania

Professor of Biochemistry & Biophysics (Secondary), Perelman School of Medicine at the University of Pennsylvania

Departments and Services

Research Interests

Metabolic disorders
Cancer metabolism
Neurochemical aberrations
Obesity
Fatty liver disease
Diabetes
Pathogenic mitochondrial integrity and function
Development of pharmacotherapeutic intervention to mitigate metabolic derangement

Publications

Papers

2018

Zhang K, Hast MW, Izumi S, Usami Y, Shetye S, Nissim I, et al. Modulating Glucose Metabolism and Lactate Synthesis in Injured Mouse Tendons: Treatment With Dichloroacetate, a Lactate Synthesis Inhibitor, Improves Tendon Healing. Am J Sports Med. 2018 Jul;46(9):2222-2231. doi: 10.1177/0363546518778789. Epub 2018 Jun 21.

Ochocki JD, Khare S, Hess M, Ackerman D, Qiu B, Nissim I, et al. Arginase 2 Suppresses Renal Carcinoma Progression via Biosynthetic Cofactor Pyridoxal Phosphate Depletion and Increased Polyamine Toxicity. Cell Metab. 2018 Jun 5;27(6):1263-1280.e6. doi: 10.1016/j.cmet.2018.04.009. Epub 2018 May 10.

2017

Ni J, Shen TD, Chen EZ, Bittinger K, Bailey A, Nissim I, et al. A role for bacterial urease in gut dysbiosis and Crohn's disease. Sci Transl Med. 2017 Nov 15;9(416). pii: eaah6888. doi: 10.1126/scitranslmed.aah6888.

Li C, Ackermann AM, Boodhansingh KE, Bhatti TR, Liu C, Nissim I, et al. Functional and Metabolomic Consequences of KATP Channel Inactivation in Human Islets. Diabetes. 2017 Jul;66(7):1901-1913. doi: 10.2337/db17-0029. Epub 2017 Apr 25.

2015

Raju K, Doulias PT, Evans P, Krizman EN, Jackson JG, Nissim I, et al. Regulation of brain glutamate metabolism by nitric oxide and S-nitrosylation. Sci Signal. 2015 Jul 7;8(384):ra68. doi: 10.1126/scisignal.aaa4312.

Shen TC, Albenberg L, Bittinger K, Chehoud C, Chen YY, Judge CA, Nissim I, et al. Engineering the gut microbiota to treat hyperammonemia.J Clin Invest. 2015 Jul 1;125(7):2841-50. doi: 10.1172/JCI79214. Epub 2015 Jun 22.

2014

Li B, Qiu B, Lee DS, Walton ZE, Ochocki JD, Nissim I, et al. Fructose-1,6-bisphosphatase opposes renal carcinoma progression. Nature. 2014 Sep 11;513(7517):251-5. doi: 10.1038/nature13557. Epub 2014 Jul 20.

Ah Mew N, McCarter R, Daikhin Y, Lichter-Konecki U, Nissim I, Yudkoff M, Tuchman M. Augmenting ureagenesis in patients with partial carbamyl phosphate synthetase 1 deficiency with N-carbamyl-L-glutamate. J Pediatr. 2014 Aug;165(2):401-403.e3. doi: 10.1016/j.jpeds.2014.04.012. Epub 2014 May 29.

Nissim I, Horyn O, Daikhin Y, Chen P, Li C, Wehrli SL, Nissim I, Yudkoff M. The molecular and metabolic influence of long term agmatine consumption. J Biol Chem. 2014 Apr 4;289(14):9710-29. doi: 10.1074/jbc.M113.544726. Epub 2014 Feb 12.

2013

Cang C, Zhou Y, Navarro B, Seo YJ, Aranda K, Nissim I, et al. mTOR regulates lysosomal ATP-sensitive two-pore Na(+) channels to adapt to metabolic state. Cell. 2013 Feb 14;152(4):778-790. doi: 10.1016/j.cell.2013.01.023. Epub 2013 Feb 7.

Li C, Liu C, Nissim I, Chen J, Chen P, Doliba N, et al. Regulation of glucagon secretion in normal and diabetic human islets by γ-hydroxybutyrate and glycine. J Biol Chem. 2013 Feb 8;288(6):3938-51. doi: 10.1074/jbc.M112.385682. Epub 2012 Dec 24.

2012

Nissim I, Horyn O, Nissim I, Daikhin Y, Wehrli SL, Yudkoff M, Matschinsky FM. Effects of a glucokinase activator on hepatic intermediary metabolism: study with 13C-isotopomer-based metabolomics. Biochem J. 2012 Jun 15;444(3):537-51. doi: 10.1042/BJ20120163.

2011

Falk MJ, Polyak E, Zhang Z, Peng M, King R, Nissim I, et al. Probucol ameliorates renal and metabolic sequelae of primary CoQ deficiency in Pdss2 mutant mice. EMBO Mol Med. 2011 Jul;3(7):410-27. doi: 10.1002/emmm.201100149. Epub 2011 Jun 8.

Nissim I, Horyn O, Nissim I, Daikhin Y, Caldovic L, Barcelona B, et al. Down-regulation of hepatic urea synthesis by oxypurines: xanthine and uric acid inhibit N-acetylglutamate synthase. J Biol Chem. 2011 Jun 24;286(25):22055-68. doi: 10.1074/jbc.M110.209023. Epub 2011 May 3.

Falk MJ, Rao M, Ostrovsky J, Daikhin E, Nissim I, Yudkoff M. Stable isotopic profiling of intermediary metabolic flux in developing and adult stage Caenorhabditis elegans. J Vis Exp. 2011 Feb 27;(48). pii: 2288. doi: 10.3791/2288.

2010

Li C, Chen P, Palladino A, Narayan S, Russell LK, Nissim I, et al. Mechanism of hyperinsulinism in short-chain 3-hydroxyacyl-CoA dehydrogenase deficiency involves activation of glutamate dehydrogenase. J Biol Chem. 2010 Oct 8;285(41):31806-18. doi: 10.1074/jbc.M110.123638. Epub 2010 Jul 29.

Ah Mew N, McCarter R, Daikhin Y, Nissim I, Yudkoff M, Tuchman M.

21.

N-carbamylglutamate augments ureagenesis and reduces ammonia and glutamine in propionic acidemia. Pediatrics. 2010 Jul;126(1):e208-14. doi: 10.1542/peds.2010-0008. Epub 2010 Jun 21.

Yudkoff M, Ah Mew N, Daikhin Y, Horyn O, Nissim I, et al. Measuring in vivo ureagenesis with stable isotopes. Mol Genet Metab. 2010;100 Suppl 1:S37-41. doi: 10.1016/j.ymgme.2010.02.017. Epub 2010 Feb 26.

Cole JT, Mitala CM, Kundu S, Verma A, Elkind JA, Nissim I, Cohen AS. Dietary branched chain amino acids ameliorate injury-induced cognitive impairment. Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):366-71. doi: 10.1073/pnas.0910280107. Epub 2009 Dec 7. Erratum in: Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):2373.

2009

Ah Mew N, Payan I, Daikhin Y, Nissim I, Nissim I, Tuchman M, Yudkoff M. Effects of a single dose of N-carbamylglutamate on the rate of ureagenesis. Mol Genet Metab. 2009 Dec;98(4):325-30. doi: 10.1016/j.ymgme.2009.07.010. Epub 2009 Jul 14. Erratum in: Mol Genet Metab. 2010 Apr;99(4):440.

da Silva RP, Nissim I, Brosnan ME, Brosnan JT. Creatine synthesis: hepatic metabolism of guanidinoacetate and creatine in the rat in vitro and in vivo. Am J Physiol Endocrinol Metab. 2009 Feb;296(2):E256-61. doi: 10.1152/ajpendo.90547.2008. Epub 2008 Nov 18.

2008

Yudkoff M, Daikhin Y, Horyn O, Nissim I, Nissim I. Ketosis and brain handling of glutamate, glutamine, and GABA. Epilepsia. 2008 Nov;49 Suppl 8:73-5. doi: 10.1111/j.1528-1167.2008.01841.x. Review.

Wise DR, DeBerardinis RJ, Mancuso A, Sayed N, Zhang XY, Nissim I, et al. Myc regulates a transcriptional program that stimulates mitochondrial glutaminolysis and leads to glutamine addiction. Proc Natl Acad Sci U S A. 2008 Dec 2;105(48):18782-7. doi: 10.1073/pnas.0810199105. Epub 2008 Nov 24.

Li C, Nissim I, Chen P, Buettger C, Najafi H, Daikhin Y, et al. Elimination of KATP channels in mouse islets results in elevated [U-13C]glucose metabolism, glutaminolysis, and pyruvate cycling but a decreased gamma-aminobutyric acid shunt. J Biol Chem. 2008 Jun 20;283(25):17238-49. doi: 10.1074/jbc.M709235200. Epub 2008 Apr 29.

Tuchman M, Caldovic L, Daikhin Y, Horyn O, Nissim I, et al. N-carbamylglutamate markedly enhances ureagenesis in N-acetylglutamate deficiency and propionic acidemia as measured by isotopic incorporation and blood biomarkers. Pediatr Res. 2008 Aug;64(2):213-7. doi: 10.1203/PDR.0b013e318179454b.

Nissim I, Horyn O, Nissim I, Daikhin Y, Wehrli SL, Yudkoff M. 3-isobutylmethylxanthine inhibits hepatic urea synthesis: protection by agmatine. J Biol Chem. 2008 May 30;283(22):15063-71. doi: 10.1074/jbc.M800163200. Epub 2008 Mar 28.

Falk MJ, Zhang Z, Rosenjack JR, Nissim I, Daikhin E, et al. Metabolic pathway profiling of mitochondrial respiratory chain mutants in C. elegans. Mol Genet Metab. 2008 Apr;93(4):388-97. doi: 10.1016/j.ymgme.2007.11.007. Epub 2008 Feb 21.

2007

DeBerardinis RJ, Mancuso A, Daikhin E, Nissim I, Yudkoff M, Wehrli S, Thompson CB. Beyond aerobic glycolysis: transformed cells can engage in glutamine metabolism that exceeds the requirement for protein and nucleotide synthesis. Proc Natl Acad Sci U S A. 2007 Dec 4;104(49):19345-50. Epub 2007 Nov 21.

Yudkoff M, Daikhin Y, Melø TM, Nissim I, Sonnewald U, Nissim I. The ketogenic diet and brain metabolism of amino acids: relationship to the anticonvulsant effect. Annu Rev Nutr. 2007;27:415-30. Review.

Turturro F, Oliver R 3rd, Friday E, Nissim I, Welbourne T. Troglitazone and pioglitazone interactions via PPAR-gamma-independent and -dependent pathways in regulating physiological responses in renal tubule-derived cell lines. Am J Physiol Cell Physiol. 2007 Mar;292(3):C1137-46. Epub 2006 Oct 25.

2006

Nissim I, Horyn O, Daikhin Y, Nissim I, Luhovyy B, Phillips PC, Yudkoff M. Ifosfamide-induced nephrotoxicity: mechanism and prevention. Cancer Res. 2006 Aug 1;66(15):7824-31.

Li C, Matter A, Kelly A, Petty TJ, Najafi H, MacMullen C, Nissim I, et al. Effects of a GTP-insensitive mutation of glutamate dehydrogenase on insulin secretion in transgenic mice. J Biol Chem. 2006 Jun 2;281(22):15064-72. Epub 2006 Mar 30.

Yudkoff M, Daikhin Y, Nissim I, Horyn O, Luhovyy B, Lazarow A, Nissim I. Short-term fasting, seizure control and brain amino acid metabolism. Neurochem Int. 2006 May-Jun;48(6-7):650-6. Epub 2006 Feb 28.

Nissim I, Daikhin Y, Nissim I, Luhovyy B, Horyn O, Wehrli SL, Yudkoff M. Agmatine stimulates hepatic fatty acid oxidation: a possible mechanism for up-regulation of ureagenesis. J Biol Chem. 2006 Mar 31;281(13):8486-96. Epub 2006 Feb 1.

2005

Yudkoff M, Daikhin Y, Nissim I, Horyn O, Luhovyy B, Luhovyy B, Lazarow A, Nissim I. Brain amino acid requirements and toxicity: the example of leucine. J Nutr. 2005 Jun;135(6 Suppl):1531S-8S. doi: 10.1093/jn/135.6.1531S. Review. Erratum in: J Nutr. 2005 Aug;135(8):2009. Luhovyy, Bogdan [corrected to Luhovyy, Bohdan].

Yudkoff M, Daikhin Y, Nissim I, Horyn O, Lazarow A, Luhovyy B, Wehrli S, Nissim I. Response of brain amino acid metabolism to ketosis. Neurochem Int. 2005 Jul;47(1-2):119-28.

Nissim I, Shohat T, Inbar Y. From dumping to sanitary landfills--solid waste management in Israel. Waste Manag. 2005;25(3):323-7. Review.

Nissim I, Luhovyy B, Horyn O, Daikhin Y, Nissim I, Yudkoff M. The role of mitochondrially bound arginase in the regulation of urea synthesis: studies with [U-15N4]arginine, isolated mitochondria, and perfused rat liver. J Biol Chem. 2005 May 6;280(18):17715-24. Epub 2005 Mar 7.

Horyn O, Luhovyy B, Lazarow A, Daikhin Y, Nissim I, Yudkoff M, Nissim I. Biosynthesis of agmatine in isolated mitochondria and perfused rat liver: studies with 15N-labelled arginine. Biochem J. 2005 Jun 1;388(Pt 2):419-25.

2004

Caldovic L, Morizono H, Daikhin Y, Nissim I, McCarter RJ, Yudkoff M, Tuchman M. Restoration of ureagenesis in N-acetylglutamate synthase deficiency by N-carbamylglutamate. J Pediatr. 2004 Oct;145(4):552-4.

Yudkoff M, Daikhin Y, Nissim I, Lazarow A, Nissim I. Ketogenic diet, brain glutamate metabolism and seizure control. Prostaglandins Leukot Essent Fatty Acids. 2004 Mar;70(3):277-85. Review.

Li C, Buettger C, Kwagh J, Matter A, Daikhin Y, Nissim IB, et al. A signaling role of glutamine in insulin secretion. J Biol Chem. 2004 Apr 2;279(14):13393-401. Epub 2004 Jan 20.

Brosnan JT, Brosnan ME, Nissim I. A window into cellular metabolism: hepatic metabolism of (15)N-labelled substrates. Metab Eng. 2004 Jan;6(1):6-11.

Kasumov T, Brunengraber LL, Comte B, Puchowicz MA, Jobbins K, Nissim I, et al. Drug Metab Dispos. 2004 Jan;32(1):10-9.

Feldkamp T, Kribben A, Roeser NF, Senter RA, Kemner S, Venkatachalam MA, Nissim I, Weinberg JM. Preservation of complex I function during hypoxia-reoxygenation-induced mitochondrial injury in proximal tubules. Am J Physiol Renal Physiol. 2004 Apr;286(4):F749-59. Epub 2003 Dec 9.

Welbourne T, Friday E, Fowler R, Turturro F, Nissim I. Troglitazone acts by PPARgamma and PPARgamma-independent pathways on LLC-PK1-F+ acid-base metabolism. Am J Physiol Renal Physiol. 2004 Jan;286(1):F100-10. Epub 2003 Sep 23.

2003

Nissim I, Horyn O, Luhovyy B, Lazarow A, Daikhin Y, Nissim I, Yudkoff M. Role of the glutamate dehydrogenase reaction in furnishing aspartate nitrogen for urea synthesis: studies in perfused rat liver with 15N. Biochem J. 2003 Nov 15;376(Pt 1):179-88.

Yudkoff M, Daikhin Y, Nissim I, Horyn O, Lazarow A, Nissim I. Metabolism of brain amino acids following pentylenetetrazole treatment. Epilepsy Res. 2003 Feb;53(1-2):151-62.

Li C, Najafi H, Daikhin Y, Nissim IB, Collins HW, Yudkoff M, Matschinsky FM, Stanley CA. Regulation of leucine-stimulated insulin secretion and glutamine metabolism in isolated rat islets. J Biol Chem. 2003 Jan 31;278(5):2853-8. Epub 2002 Nov 19.

2002

Nissim I, Horyn O, Daikhin Y, Nissim I, Lazarow A, Yudkoff M. Regulation of urea synthesis by agmatine in the perfused liver: studies with 15N. Am J Physiol Endocrinol Metab. 2002 Dec;283(6):E1123-34. Epub 2002 Aug 13.

Coates G, Nissim I, Battarbee H, Welbourne T. Glitazones regulate glutamine metabolism by inducing a cellular acidosis in MDCK cells. Am J Physiol Endocrinol Metab. 2002 Oct;283(4):E729-37.

Brosnan JT, Brosnan ME, Nissim I. Application of 15N to the study of hepatic nitrogen metabolism. Adv Enzyme Regul. 2002;42:305-15.

Comte B, Kasumov T, Pierce BA, Puchowicz MA, Scott ME, Dahms W, Kerr D, Nissim I, Brunengraber H. Identification of phenylbutyrylglutamine, a new metabolite of phenylbutyrate metabolism in humans. J Mass Spectrom. 2002 Jun;37(6):581-90.

2001

Yudkoff M, Daikhin Y, Nissim I, Lazarow A, Nissim I. Ketogenic diet, amino acid metabolism, and seizure control. J Neurosci Res. 2001 Dec 1;66(5):931-40. Review.

Yudkoff M, Daikhin Y, Nissim I, Lazarow A, Nissim I. Brain amino acid metabolism and ketosis. J Neurosci Res. 2001 Oct 15;66(2):272-81.

Welbourne T, Routh R, Yudkoff M, Nissim I. The glutamine/glutamate couplet and cellular function. News Physiol Sci. 2001 Aug;16:157-60.

Brosnan JT, Brosnan ME, Yudkoff M, Nissim I, Daikhin Y, Lazarow A, Horyn O, Nissim I. Alanine metabolism in the perfused rat liver. Studies with (15)N. J Biol Chem. 2001 Aug 24;276(34):31876-82. Epub 2001 Jun 21.

Weinberg JM, Venkatachalam MA, Roeser NF, Senter RA, Nissim I. Energetic determinants of tyrosine phosphorylation of focal adhesion proteins during hypoxia/reoxygenation of kidney proximal tubules. Am J Pathol. 2001 Jun;158(6):2153-64.

Welbourne T, Nissim I. Regulation of mitochondrial glutamine/glutamate metabolism by glutamate transport: studies with (15)N. Am J Physiol Cell Physiol. 2001 May;280(5):C1151-9.

Weinberg JM, Venkatachalam MA, Roeser NF, Saikumar P, Dong Z, Senter RA, Nissim I. Anaerobic and aerobic pathways for salvage of proximal tubules from hypoxia-induced mitochondrial injury. Am J Physiol Renal Physiol. 2000 Nov;279(5):F927-43.

2000

Yudkoff M, Daikhin Y, Nissim I, Nissim I. Acidosis and astrocyte amino acid metabolism. Neurochem Int. 2000 Apr;36(4-5):329-39.

Weinberg JM, Venkatachalam MA, Roeser NF, Nissim I. Mitochondrial dysfunction during hypoxia/reoxygenation and its correction by anaerobic metabolism of citric acid cycle intermediates. Proc Natl Acad Sci U S A. 2000 Mar 14;97(6):2826-31.

Nissim I, Starr SE, Sullivan KE, Campbell DE, Douglas SD, Daikhin Y, Yudkoff M. Rapid method for determining the rate of DNA synthesis and cellular proliferation. Anal Biochem. 2000 Feb 15;278(2):198-205.

1999

Nissim I. Newer aspects of glutamine/glutamate metabolism: the role of acute pH changes. Am J Physiol. 1999 Oct;277(4):F493-7. doi: 10.1152/ajprenal.1999.277.4.F493. Review.

Nissim I, Brosnan ME, Yudkoff M, Brosnan JT. Studies of hepatic glutamine metabolism in the perfused rat liver with (15)N-labeled glutamine. J Biol Chem. 1999 Oct 8;274(41):28958-65.

1997

Berry GT, Nissim I, Gibson JB, Mazur AT, Lin Z, Elsas LJ, Singh RH, Klein PD, Segal S. Quantitative assessment of whole body galactose metabolism in galactosemic patients. Eur J Pediatr. 1997 Aug;156 Suppl 1:S43-9.

Yudkoff M, Daikhin Y, Nissim I, Grunstein R, Nissim I. Effects of ketone bodies on astrocyte amino acid metabolism. J Neurochem. 1997 Aug;69(2):682-92.

Brosnan JT, Brosnan ME, Charron R, Nissim I. Incorporation of 15N-labelled precursors into urea isotopomers. Contrib Nephrol. 1997;121:156-62.

1996

Nissim I, Yudkoff M, Brosnan JT. Regulation of [15N]urea synthesis from [5-15N]glutamine. Role of pH, hormones, and pyruvate. J Biol Chem. 1996 Dec 6;271(49):31234-42.

Yudkoff M, Daikhin Y, Nissim I, Jawad A, Wilson J, Batshaw M. In vivo nitrogen metabolism in ornithine transcarbamylase deficiency. J Clin Invest. 1996 Nov 1;98(9):2167-73.

Brosnan JT, Brosnan ME, Charron R, Nissim I. A mass isotopomer study of urea and glutamine synthesis from 15N-labeled ammonia in the perfused rat liver. J Biol Chem. 1996 Jul 5;271(27):16199-207.

Tannen RL, Nissim I, Sahi A. Hormonal mediators of ammoniagenesis: mechanism of action of PGF2 alpha and the implications for other hormones. Kidney Int. 1996 Jul;50(1):15-25. Review.

Yudkoff M, Daikhin Y, Nelson D, Nissim I, Erecińska M. Neuronal metabolism of branched-chain amino acids: flux through the aminotransferase pathway in synaptosomes. J Neurochem. 1996 May;66(5):2136-45.

Nissim I, Weinberg JM. Glycine attenuates Fanconi syndrome induced by maleate or ifosfamide in rats. Kidney Int. 1996 Mar;49(3):684-95.

Yudkoff M, Daikhin Y, Grunstein L, Nissim I, Stern J, Pleasure D, Nissim I. Astrocyte leucine metabolism: significance of branched-chain amino acid transamination. J Neurochem. 1996 Jan;66(1):378-85.

1995

Berry GT, Nissim I, Mazur AT, Elsas LJ, Singh RH, Klein PD, Gibson JB, Lin Z, Segal S. In vivo oxidation of [13C]galactose in patients with galactose-1-phosphate uridyltransferase deficiency. Biochem Mol Med. 1995 Dec;56(2):158-65.

Berry GT, Nissim I, Lin Z, Mazur AT, Gibson JB, Segal S. Endogenous synthesis of galactose in normal men and patients with hereditary galactosaemia. Lancet. 1995 Oct 21;346(8982):1073-4.

Foreman JW, Benson LL, Wellons M, Avner ED, Sweeney W, Nissim I, Nissim I. Metabolic studies of rat renal tubule cells loaded with cystine: the cystine dimethylester model of cystinosis. J Am Soc Nephrol. 1995 Aug;6(2):269-72.

Sahai A, Nissim I, Sandler RS, Tannen RL. Prostaglandin F2 alpha- and 12-O-tetradecanoylphorbol-13-acetate-induced alterations in the pathways of renal ammoniagenesis. J Am Soc Nephrol. 1995 Apr;5(10):1792-8.

Yudkoff M, Nissim I. Stable isotopes as metabolic probes for nutritional studies in neonates. Clin Perinatol. 1995 Mar;22(1):97-109. Review.

Nissim I, States B, Nissim I, Lin ZP, Yudkoff M. Hormonal regulation of glutamine metabolism by OK cells. Kidney Int. 1995 Jan;47(1):96-105.

1994

Yudkoff M, Daikhin Y, Nissim I, Pleasure D, Stern J, Nissim I. Inhibition of astrocyte glutamine production by alpha-ketoisocaproic acid. J Neurochem. 1994 Oct;63(4):1508-15.

Erecińska M, Nelson D, Nissim I, Daikhin Y, Yudkoff M. Cerebral alanine transport and alanine aminotransferase reaction: alanine as a source of neuronal glutamate. J Neurochem. 1994 May;62(5):1953-64.

Nissim I, Sahai A, Sandler RS, Tannen RL. The intensity of acidosis differentially alters the pathways of ammoniagenesis in LLC-PK1 cells. Kidney Int. 1994 Apr;45(4):1014-9.

Yudkoff M, Daikhin Y, Lin ZP, Nissim I, Stern J, Pleasure D, Nissim I. Interrelationships of leucine and glutamate metabolism in cultured astrocytes. J Neurochem. 1994 Mar;62(3):1192-202.

Nissim I, States B, Nissim I, Lin ZP, Yudkoff M. pH and hormonal regulation of ammoniagenesis in cultured OK cells. Contrib Nephrol. 1994;110:30-40.

Nissim I, Sahai A, Tannen RL. Effect of acute acidosis on the pathways of ammoniagenesis in LLC-PK1 cells. Contrib Nephrol. 1994;110:25-9.

1993

Nissim I, States B, Hardy M, Pleasure J, Nissim I. Effect of glutamine on heat-shock-induced mRNA and stress proteins. J Cell Physiol. 1993 Nov;157(2):313-8.

Erecińska M, Pleasure D, Nelson D, Nissim I, Yudkoff M. Cerebral aspartate utilization: near-equilibrium relationships in aspartate aminotransferase reaction. J Neurochem. 1993 May;60(5):1696-706.

Nissim I, Cattano C, Lin Z, Nissim I. Acid-base regulation of hepatic glutamine metabolism and ureagenesis: study with 15N. J Am Soc Nephrol. 1993 Jan;3(7):1416-27.

Yudkoff M, Nissim I, Daikhin Y, Lin ZP, Nelson D, Pleasure D, Erecinska M. Brain glutamate metabolism: neuronal-astroglial relationships. Dev Neurosci. 1993;15(3-5):343-50. Review.

1992

Nissim I, Hardy M, Pleasure J, Nissim I, States B. A mechanism of glycine and alanine cytoprotective action: stimulation of stress-induced HSP70 mRNA. Kidney Int. 1992 Sep;42(3):775-82. Erratum in: Kidney Int 1992 Dec;42(6):1488.

Nissim I, Cattano C, Nissim I, Yudkoff M. Relative role of the glutaminase, glutamate dehydrogenase, and AMP-deaminase pathways in hepatic ureagenesis: studies with 15N. Arch Biochem Biophys. 1992 Feb 1;292(2):393-401.

Yudkoff M, Nissim I, Hertz L, Pleasure D, Erecinska M. Nitrogen metabolism: neuronal-astroglial relationships. Prog Brain Res. 1992;94:213-24. Review.

1991

Sahai A, Nissim I, Tannen RL. Pathways of acute pH regulation of ammoniagenesis in LLC-PK1 cells: study with [15N]glutamine. Am J Physiol. 1991 Sep;261(3 Pt 2):F481-7.

Nissim I, Wehrli S, States B, Nissim I, Yudkoff M. Analysis and physiological implications of renal 2-oxoglutaramate metabolism. Biochem J. 1991 Jul 1;277 ( Pt 1):33-8.

Yudkoff M, Nissim I, Nelson D, Lin ZP, Erecińska M. Glutamate dehydrogenase reaction as a source of glutamic acid in synaptosomes. J Neurochem. 1991 Jul;57(1):153-60.

Weinberg JM, Nissim I, Roeser NF, Davis JA, Schultz S, Nissim I. Relationships between intracellular amino acid levels and protection against injury to isolated proximal tubules. Am J Physiol. 1991 Mar;260(3 Pt 2):F410-9.

Nissim I, Nissim I, Yudkoff M. Adaptation of renal tricarboxylic acid cycle metabolism to various acid-base states: study with [3-13C,5-15N]glutamine. Miner Electrolyte Metab. 1991;17(1):21-31.

Nissim I, States B, Nissim I, Yudkoff M. The role of gamma-glutamyl transpeptidase in glutamine metabolism by cultured human kidney cells. Contrib Nephrol. 1991;92:191-9.

1990

Yudkoff M, Nissim I, Hertz L. Precursors of glutamic acid nitrogen in primary neuronal cultures: studies with 15N. Neurochem Res. 1990 Dec;15(12):1191-6.

Yudkoff M, Pleasure D, Cregar L, Lin ZP, Nissim I, Stern J, Nissim I. Glutathione turnover in cultured astrocytes: studies with [15N]glutamate. J Neurochem. 1990 Jul;55(1):137-45.

Erecińska M, Zaleska MM, Nelson D, Nissim I, Yudkoff M. Neuronal glutamine utilization: glutamine/glutamate homeostasis in synaptosomes. J Neurochem. 1990 Jun;54(6):2057-69.

Nissim I, Nissim I, Yudkoff M. Carbon flux through tricarboxylic acid cycle in rat renal tubules. Biochim Biophys Acta. 1990 Feb 26;1033(2):194-200.

Nissim I, Korzets Z. Ammonia metabolism in cultured renal cells. Miner Electrolyte Metab. 1990;16(5):270-6. Review.

1989

Yudkoff M, Zaleska MM, Nissim I, Nelson D, Erecińska M. Neuronal glutamine utilization: pathways of nitrogen transfer studied with [15N]glutamine. J Neurochem. 1989 Aug;53(2):632-40.

Yudkoff M, Nissim I, Nissim I, Stern J, Pleasure D. Effects of palmitate on astrocyte amino acid contents. Neurochem Res. 1989 Apr;14(4):367-70.

Nissim I, Yudkoff M, Nissim I, States B. Effect of 5-amino-4-imidazolecarboxamide riboside (AICA-riboside) on the purine nucleotide synthesis and growth of rat kidney cells in culture: study with [15N]aspartate. J Cell Physiol. 1989 Mar;138(3):536-40.

Polin RA, Yoder MC, Douglas SD, McNelis W, Nissim I, Yudkoff M. Fibronectin turnover in the premature neonate measured with [15N]glycine. Am J Clin Nutr. 1989 Feb;49(2):314-9.

Nissim I, States B. Ammoniagenesis by cultured human renal cortical epithelial cells: study with 15N. Am J Physiol. 1989 Jan;256(1 Pt 2):F187-96.

1988

Erecińska M, Zaleska MM, Nissim I, Nelson D, Dagani F, Yudkoff M. Glucose and synaptosomal glutamate metabolism: studies with [15N]glutamate. J Neurochem. 1988 Sep;51(3):892-902.

Yudkoff M, Nissim I, Pleasure D. Astrocyte metabolism of [15N]glutamine: implications for the glutamine-glutamate cycle. J Neurochem. 1988 Sep;51(3):843-50.

Nissim I, Yudkoff M, Segal S. Interrelationships between renal TCA-cycle metabolism and ammoniagenesis: studies with 13C-NMR and GC-MS. Contrib Nephrol. 1988;63:60-70.

1987

Nissim I, States B, Yudkoff M, Segal S. Characterization of amino acid metabolism by cultured rat kidney cells: study with 15N. Am J Physiol. 1987 Dec;253(6 Pt 2):F1243-52. Erratum in: Am J Physiol 1988 Mar;254(3 Pt 2):F296.

Nissim I, Yudkoff M, Segal S. Metabolic fate of glutamate carbon in rat renal tubules. Studies with 13C nuclear magnetic resonance and gas chromatography-mass spectrometry. Biochem J. 1987 Jan 15;241(2):361-70.

Yudkoff M, Nissim I, McNellis W, Polin R. Albumin synthesis in premature infants: determination of turnover with [15N]glycine. Pediatr Res. 1987 Jan;21(1):49-53.

Yudkoff M, Nissim I, Pleasure D. [15N]aspartate metabolism in cultured astrocytes. Studies with gas chromatography-mass spectrometry. Biochem J. 1987 Jan 1;241(1):193-201.

1986

Nissim I, Yudkoff M, Segal S. Nitrogen sources for renal ammoniagenesis: study with 15N amino acid. Am J Physiol. 1986 Dec;251(6 Pt 2):F995-1002.

Nissim I, Yudkoff M, Segal S. Effect of 5-amino-4-imidazolecarboxamide riboside on renal ammoniagenesis. Study with [15N]aspartate. J Biol Chem. 1986 May 15;261(14):6509-14.

Yudkoff M, Nissim I. Methods for determining the protein requirement of infants. Clin Perinatol. 1986 Mar;13(1):123-32. Review.

Yudkoff M, Nissim I, Hummeler K, Medow M, Pleasure D. Utilization of [15N]glutamate by cultured astrocytes. Biochem J. 1986 Feb 15;234(1):185-92.

Nissim I, Lapidot A. Dynamic aspects of amino acid metabolism in alloxan-induced diabetes and insulin-treated rabbits: in vivo studies with 15N and gas chromatography-mass spectrometry. Biochem Med Metab Biol. 1986 Feb;35(1):88-100.

1985

Nissim I, Yudkoff M, Segal S. Metabolism of glutamine and glutamate by rat renal tubules. Study with 15N and gas chromatography-mass spectrometry. J Biol Chem. 1985 Nov 15;260(26):13955-67.

Yudkoff M, Nissim I, Pleasure D, Hummeler K, Segal S. Application of gas chromatography-mass spectrometry to in vitro studies of nitrogen metabolism. Contrib Nephrol. 1985;47:209-18.

1984

Nissim I, Yudkoff M, Lapidot A. Simultaneous determination of [2-15N]- and [5-15N]glutamine with gas chromatography-mass spectroscopy: applications to nitrogen metabolic studies. Anal Biochem. 1984 Nov 15;143(1):14-20.

Yudkoff M, Nissim I, Pereira G, Segal S. Urinary excretion of dimethylarginines in premature infants. Biochem Med. 1984 Oct;32(2):242-51.

Nissim I, Lapidot A. The roles of insulin and glucagon in the regulation of amino acid turnover rate and pool size: in vivo study with [15N]glycine and gas chromatography--mass spectrometry. Biochem Med. 1984 Apr;31(2):185-200.

Yudkoff M, Nissim I, Glassman M, Segal S. Whole body nitrogen kinetics in man: determination from plasma [guanidino-15N]arginine. Clin Sci (Lond). 1984 Mar;66(3):337-42.

Lapidot A, Nissim I, Shaklai M, Samuel R, Liberman UA. Glycine pools and turnover rates in leukaemia patients measured with [15N]glycine. Clin Sci (Lond). 1984 Feb;66(2):147-53.

Yudkoff M, Nissim I, Kim SU, Pleasure D, Segal S. Metabolism of 15NH3 in organotypic cerebellar explants and cultured astrocytes: studies with gas chromatography-mass spectrometry. J Neurochem. 1984 Jan;42(1):283-6.

Yudkoff M, Nissim I, Stanley C, Baker L, Segal S. Glycogen storage disease: effects of glucose infusions on [15N]glycine kinetics and nitrogen metabolism. J Pediatr Gastroenterol Nutr. 1984;3(1):81-8.

1983

Yudkoff M, Nissim I, Kim S, Pleasure D, Hummeler K, Segal S. [15N] leucine as a source of [15N] glutamate in organotypic cerebellar explants. Biochem Biophys Res Commun. 1983 Aug 30;115(1):174-9.

Nissim I, Yudkoff M, Segal S. A model for determination of total body protein synthesis based upon compartmental analysis of the plasma [15N] glycine decay curve. Metabolism. 1983 Jul;32(7):646-53.

Nissim I, Yudkoff M, Terwilliger T, Segal S. Rapid determination of [guanidino-15N]arginine in plasma with gas chromatography--mass spectrometry: application to human metabolic studies. Anal Biochem. 1983 May;131(1):75-82.

Nissim I, Yudkoff M, Pereira G, Segal S. Effects of conceptual age and dietary intake on protein metabolism in premature infants. J Pediatr Gastroenterol Nutr. 1983;2(3):507-16.

1982

Yudkoff M, Nissim I, Segal S. Determination of [amide-15N]glutamine in plasma with gas chromatography-mass spectrometry. Clin Chim Acta. 1982 Feb 5;118(2-3):159-66.

1981

Yudkoff M, Nissim I, Schneider A, Segal S. Cysteamine inhibition of [15N]-glycine turnover in cystinosis and of glycine cleavage system in vitro. Metabolism. 1981 Nov;30(11):1096-103.

Nissim I, Yudkoff M, Yang W, Terwilliger T, Segal S. Gas chromatography-mass spectrometry determination of [15N]ammonia enrichment in blood and urine. Anal Biochem. 1981 Jun;114(1):125-30.

Nissim I, Yudkoff M, Yang W, Terwilliger T, Segal S. Rapid gas chromatographic-mass spectrometric analysis of [15N]urea: application to human metabolic studies. Clin Chim Acta. 1981 Feb 5;109(3):295-304.

1980

Lapidot A, Nissim I. Regulation of pool sizes and turnover rates of amino acids in humans: 15N-glycine and 15N-alanine single-dose experiments using gas chromatography-mass spectrometry analysis. Metabolism. 1980 Mar;29(3):230-9.

1979

Nissim I, Lapidot A. Plasma amino acid turnover rates and pools in rabbits: in vivo studies using stable isotopes. Am J Physiol. 1979 Nov;237(5):E418-27.

1978

Irving CS, Nissim I, Lapidot A. The determination of amino acid pool sizes and turnover rates by gas chromatographic mass spectrometric analysis of stable isotope enrichment. Biomed Mass Spectrom. 1978 Feb;5(2):117-22.

Irving CS, Nissim I, Lapidot A. Whole body amino acid pools and turnover rates determined by a stable isotope gas chromatographic mass spectrometric methodology. Monogr Hum Genet. 1978;9:50-7.

Awards and Honors

2002, Research Recognition Award, The Leukemia & Lymphoma Society, Penn. Chapter
1995, Elected to New York Academy of Sciences
1991, Established Investigator Award, American Heart Association
1991, Research Achievement Award, Joseph Stokes Research Institute, Children's Hospital of Philadelphia
1979, International Union Against Cancer on International Cancer Research Technology Transfer Award

Leadership and Memberships

Memberships in Professional Organizations

International

1988-present, International Society of Nephrology

National

1998-present, American Physiologic Society
1997-present, American Society for Biochemistry and Molecular Biology
1990-present, American Chemical Society
1985-present, American Society of Nephrology