Bryan A. Wolf, MD, PhD

male silhouette icon
Bryan A. Wolf, MD, PhD, is Chief Scientific Officer and Chair of the Department of Biomedical and Health Informatics at Children's Hospital of Philadelphia. He holds the Edmond F. Notebaert Endowed Chair in Pediatric Research.

Locations: Main Campus
Appointments and Referrals: 1-800-TRY-CHOP (1-800-879-2467)

Background

Bryan Wolf, MD, PhD, has an outstanding record of creative thought leadership in research at Children’s Hospital of Philadelphia and the Perelman School of Medicine at the University of Pennsylvania. His early career as a diabetes researcher evolved into several hospital, administrative, and faculty appointments on the CHOP/Penn campus since 1990, including serving as pathologist-in-chief and chair of CHOP’s Department of Pathology and Laboratory Medicine from 2001 to 2008. Dr. Wolf held the positions of CHOP’s chief information officer and senior vice president from 2008 to 2015. He was named the Research Institute’s Executive Vice President and Chief Scientific Officer in 2015 and began leading, mentoring, and recruiting a skilled team of researchers and administration staff with a wide range of expertise that is devoted to performing the best research that improves the health of children.

In his role as Chair, Department of Biomedical and Health Informatics (DBHi), Dr. Wolf oversees various programs, applications, research, and services to explore how technology can impact both research and patient care. The department’s talented team of data scientists, programmers, and bioinformatics scientists combine technological and scientific expertise to transform research and clinical data into innovative solutions that directly impact patient outcomes. Additionally, DBHi provides an academic home for all research informatics activities, including the development and deployment of intellectual, technical, and educational resources in biomedical computing. Under the purview of the DBHi, the Arcus, Bioinformatics, Data Reporting and Management, Application Development, and Education groups help translate big data into healthcare discoveries.

Education and Training

Medical School

MD - University of Paris Reni-Descartes School of Medicine Necker-Enfants-Malades, Paris, France

Residency

Laboratory Medicine - Washington University School of Medicine, St. Louis, MO

Fellowship

Post doctoral Research Fellow in Biochemistry - Washington University School of Medicine, St. Louis, MO

Board Certification

Clinical Pathology

Graduate Degree

PhD in Nutrition/Endocrinology - University of Paris Jussieu, Paris, France
MA (Honorary) - University of Pennsylvania, Philadelphia, PA

Titles and Academic Titles

Executive Vice President and Chief Scientific Officer

Chair, Department of Biomedical and Health Informatics

Edmond F. Notebaert Endowed Chair in Pediatric Research

Professor of Pathology and Laboratory Medicine, Perelman School of Medicine at the University of Pennsylvania

Departments and Services

Publications

Papers

2014

Forrest CB, Margolis PA, Bailey LC, Marsolo K, Del Beccaro MA, Finkelstein JA, Milov DE, Vieland VJ, Wolf BA, Yu FB, Kahn MG. PEDSnet: a National Pediatric Learning Health System. J Am Med Inform Assoc. 2014 Jul-Aug;21(4):602-6. doi: 10.1136/amiajnl-2014-002743. Epub 2014 May 12. PubMed PMID: 24821737; PubMed Central PMCID: PMC4078288.

2012

Talbot K, Wang HY, Kazi H, Han LY, Bakshi KP, Stucky A, Fuino RL, Kawaguchi KR, Samoyedny AJ, Wilson RS, Arvanitakis Z, Schneider JA, Wolf BA, Bennett DA, Trojanowski JQ, Arnold SE. Demonstrated brain insulin resistance in Alzheimer's disease patients is associated with IGF-1 resistance, IRS-1 dysregulation, and cognitive decline. J Clin Invest. 2012 Apr;122(4):1316-38. doi: 10.1172/JCI59903. PubMed PMID: 22476197; PubMed Central PMCID: PMC3314463.

2010

Wilson CG, Schupp M, Burkhardt BR, Wu J, Young RA, Wolf BA. Liver-specific overexpression of pancreatic-derived factor (PANDER) induces fasting hyperglycemia in mice. Endocrinology. 2010 Nov;151(11):5174-84. doi: 10.1210/en.2010-0379. Epub 2010 Sep 15. PubMed PMID: 20844005; PubMed Central PMCID: PMC2954722.

Robert-Cooperman CE, Carnegie JR, Wilson CG, Yang J, Cook JR, Wu J, Young RA, Wolf BA, Burkhardt BR. Targeted disruption of pancreatic-derived factor (PANDER, FAM3B) impairs pancreatic beta-cell function. Diabetes. 2010 Sep;59(9):2209-18. doi: 10.2337/db09-1552. Epub 2010 Jun 21. PubMed PMID: 20566664; PubMed Central PMCID: PMC2927943.

Carnegie JR, Robert-Cooperman CE, Wu J, Young RA, Wolf BA, Burkhardt BR. Characterization of the expression, localization, and secretion of PANDER in alpha-cells. Mol Cell Endocrinol. 2010 Aug 30;325(1-2):36-45. doi: 10.1016/j.mce.2010.05.008. PubMed PMID: 20638985; PubMed Central PMCID: PMC2908920.

Yang J, Chi Y, Burkhardt BR, Guan Y, Wolf BA. Leucine metabolism in regulation of insulin secretion from pancreatic beta cells. Nutr Rev. 2010 May;68(5):270-9. doi: 10.1111/j.1753-4887.2010.00282.x. Review. PubMed PMID: 20500788; PubMed Central PMCID: PMC2969169.

2009

Yang J, Wang C, Li J, Burkhardt BR, Robert-Cooperman CE, Wilson C, Gao Z, Wolf BA. PANDER binds to the liver cell membrane and inhibits insulin signaling in HepG2 cells. FEBS Lett. 2009 Sep 17;583(18):3009-15. doi: 10.1016/j.febslet.2009.08.008. Epub 2009 Aug 14. PubMed PMID: 19683528.

2008

Burkhardt BR, Cook JR, Young RA, Wolf BA. PDX-1 interaction and regulation of the Pancreatic Derived Factor (PANDER, FAM3B) promoter. Biochim Biophys Acta. 2008 Oct;1779(10):645-51. doi: 10.1016/j.bbagrm.2008.07.007. Epub 2008 Jul 29. PubMed PMID: 18708173; PubMed Central PMCID: PMC2574553.

2006

Xiong X, Eckelt J, Wolf BA, Zhang Z, Zhang L. Continuous spin fractionation and characterization by size-exclusion chromatography for styrene-butadiene block copolymers. J Chromatogr A. 2006 Mar 31;1110(1-2):53-60. doi: 10.1016/j.chroma.2006.01.063. Epub 2006 Feb 8. PubMed PMID: 16466731.

Burkhardt BR, Greene SR, White P, Wong RK, Brestelli JE, Yang J, Robert CE, Brusko TM, Wasserfall CH, Wu J, Atkinson MA, Gao Z, Kaestner KH, Wolf BA. PANDER-induced cell-death genetic networks in islets reveal central role for caspase-3 and cyclin-dependent kinase inhibitor 1A (p21). Gene. 2006 Mar 15;369:134-41. doi: 10.1016/j.gene.2005.10.040. Epub 2006 Jan 18. PubMed PMID: 16412588.

Yang J, Wong RK, Park M, Wu J, Cook JR, York DA, Deng S, Markmann J, Naji A, Wolf BA, Gao Z. Leucine regulation of glucokinase and ATP synthase sensitizes glucose-induced insulin secretion in pancreatic beta-cells. Diabetes. 2006 Jan;55(1):193-201. PubMed PMID: 16380493.

2005

Cao X, Yang J, Burkhardt BR, Gao Z, Wong RK, Greene SR, Wu J, Wolf BA. Effects of overexpression of pancreatic derived factor (FAM3B) in isolated mouse islets and insulin-secreting betaTC3 cells. Am J Physiol Endocrinol Metab. 2005 Oct;289(4):E543-50. doi: 10.1152/ajpendo.00113.2005. Epub 2005 May 31. PubMed PMID: 15928025.

Burkhardt BR, Yang MC, Robert CE, Greene SR, McFadden KK, Yang J, Wu J, Gao Z, Wolf BA. Tissue-specific and glucose-responsive expression of the pancreatic derived factor (PANDER) promoter. Biochim Biophys Acta. 2005 Sep 25;1730(3):215-25. doi: 10.1016/j.bbaexp.2005.07.003. PubMed PMID: 16102856.

Yang J, Gao Z, Robert CE, Burkhardt BR, Gaweska H, Wagner A, Wu J, Greene SR, Young RA, Wolf BA. Structure-function studies of PANDER, an islet specific cytokine inducing cell death of insulin-secreting beta cells. Biochemistry. 2005 Aug 30;44(34):11342-52. doi: 10.1021/bi0503908. PubMed PMID: 16114871.

Xu W, Gao Z, Wu J, Wolf BA. Interferon-gamma-induced regulation of the pancreatic derived cytokine FAM3B in islets and insulin-secreting betaTC3 cells. Mol Cell Endocrinol. 2005 Aug 30;240(1-2):74-81. doi: 10.1016/j.mce.2005.05.010. PubMed PMID: 16006032.

2004

Yang J, Wong RK, Wang X, Moibi J, Hessner MJ, Greene S, Wu J, Sukumvanich S, Wolf BA, Gao Z. Leucine culture reveals that ATP synthase functions as a fuel sensor in pancreatic beta-cells. J Biol Chem. 2004 Dec 24;279(52):53915-23. doi: 10.1074/jbc.M405309200. Epub 2004 Oct 14. PubMed PMID: 15489222.

Guo CJ, Douglas SD, Gao Z, Wolf BA, Grinspan J, Lai JP, Riedel E, Ho WZ. Interleukin-1beta upregulates functional expression of neurokinin-1 receptor (NK-1R) via NF-kappaB in astrocytes. Glia. 2004 Nov 15;48(3):259-66. doi: 10.1002/glia.20079. PubMed PMID: 15390113; PubMed Central PMCID: PMC4016813.

Schmitz A, Shiue CY, Feng Q, Shiue GG, Deng S, Pourdehnad MT, Schirrmacher R, Vatamaniuk M, Doliba N, Matschinsky F, Wolf B, Rösch F, Naji A, Alavi AA. Synthesis and evaluation of fluorine-18 labeled glyburide analogs as beta-cell imaging agents. Nucl Med Biol. 2004 May;31(4):483-91. doi: 10.1016/j.nucmedbio.2003.12.003. PubMed PMID: 15093819.

Deng S, Vatamaniuk M, Huang X, Doliba N, Lian MM, Frank A, Velidedeoglu E, Desai NM, Koeberlein B, Wolf B, Barker CF, Naji A, Matschinsky FM, Markmann JF. Structural and functional abnormalities in the islets isolated from type 2 diabetic subjects. Diabetes. 2004 Mar;53(3):624-32. doi: 10.2337/diabetes.53.3.624. PubMed PMID: 14988246.

2003

Desai NM, Goss JA, Deng S, Wolf BA, Markmann E, Palanjian M, Shock AP, Feliciano S, Brunicardi FC, Barker CF, Naji A, Markmann JF. Elevated portal vein drug levels of sirolimus and tacrolimus in islet transplant recipients: local immunosuppression or islet toxicity?. Transplantation. 2003 Dec 15;76(11):1623-5. doi: 10.1097/01.TP.0000081043.23751.81. PubMed PMID: 14702535.

Bell E, Cao X, Moibi JA, Greene SR, Young R, Trucco M, Gao Z, Matschinsky FM, Deng S, Markman JF, Naji A, Wolf BA. Rapamycin has a deleterious effect on MIN-6 cells and rat and human islets. Diabetes. 2003 Nov;52(11):2731-9. doi: 10.2337/diabetes.52.11.2731. PubMed PMID: 14578291.

Cao X, Gao Z, Robert CE, Greene S, Xu G, Xu W, Bell E, Campbell D, Zhu Y, Young R, Trucco M, Markmann JF, Naji A, Wolf BA. Pancreatic-derived factor (FAM3B), a novel islet cytokine, induces apoptosis of insulin-secreting beta-cells. Diabetes. 2003 Sep;52(9):2296-303. doi: 10.2337/diabetes.52.9.2296. PubMed PMID: 12941769.

Markmann JF, Deng S, Huang X, Desai NM, Velidedeoglu EH, Lui C, Frank A, Markmann E, Palanjian M, Brayman K, Wolf B, Bell E, Vitamaniuk M, Doliba N, Matschinsky F, Barker CF, Naji A. Insulin independence following isolated islet transplantation and single islet infusions. Ann Surg. 2003 Jun;237(6):741-9; discussion 749-50. doi: 10.1097/01.SLA.0000072110.93780.52. PubMed PMID: 12796569; PubMed Central PMCID: PMC1514687.

Markmann JF, Deng S, Desai NM, Huang X, Velidedeoglu E, Frank A, Liu C, Brayman KL, Lian MM, Wolf B, Bell E, Vitamaniuk M, Doliba N, Matschinsky F, Markmann E, Barker CF, Naji A. The use of non-heart-beating donors for isolated pancreatic islet transplantation. Transplantation. 2003 May 15;75(9):1423-9. doi: 10.1097/01.TP.0000061119.32575.F4. PubMed PMID: 12792491.

Gao Z, Young RA, Li G, Najafi H, Buettger C, Sukumvanich SS, Wong RK, Wolf BA, Matschinsky FM. Distinguishing features of leucine and alpha-ketoisocaproate sensing in pancreatic beta-cells. Endocrinology. 2003 May;144(5):1949-57. doi: 10.1210/en.2002-0072. PubMed PMID: 12697702.

Borge PD Jr, Wolf BA. Insulin receptor substrate 1 regulation of sarco-endoplasmic reticulum calcium ATPase 3 in insulin-secreting beta-cells. J Biol Chem. 2003 Mar 28;278(13):11359-68. doi: 10.1074/jbc.M209521200. Epub 2003 Jan 10. PubMed PMID: 12524443.

Deng S, Vatamaniuk M, Lian MM, Doliba N, Wang J, Bell E, Wolf B, Raper S, Matschinsky FM, Markmann JF. Insulin gene transfer enhances the function of human islet grafts. Diabetologia. 2003 Mar;46(3):386-93. doi: 10.1007/s00125-003-1038-3. Epub 2003 Mar 7. PubMed PMID: 12687337.

2002

Westerlund J, Wolf BA, Bergsten P. Glucose-dependent promotion of insulin release from mouse pancreatic islets by the insulin-mimetic compound L-783,281. Diabetes. 2002 Feb;51 Suppl 1:S50-2. doi: 10.2337/diabetes.51.2007.s50. PubMed PMID: 11815458.

Sudoh S, Frosch MP, Wolf BA. Differential effects of proteases involved in intracellular degradation of amyloid beta-protein between detergent-soluble and -insoluble pools in CHO-695 cells. Biochemistry. 2002 Jan 29;41(4):1091-9. doi: 10.1021/bi011193l. PubMed PMID: 11802707.

2001

Major CD, Wolf BA. Interleukin-1beta stimulation of c-Jun NH(2)-terminal kinase activity in insulin-secreting cells: evidence for cytoplasmic restriction. Diabetes. 2001 Dec;50(12):2721-8. doi: 10.2337/diabetes.50.12.2721. PubMed PMID: 11723054.

2000

Jolly-Tornetta C, Wolf BA. Protein kinase C regulation of intracellular and cell surface amyloid precursor protein (APP) cleavage in CHO695 cells. Biochemistry. 2000 Dec 12;39(49):15282-90. doi: 10.1021/bi001723y. PubMed PMID: 11106509.

Xu GG, Gao ZY, Borge PD Jr, Jegier PA, Young RA, Wolf BA. Insulin regulation of beta-cell function involves a feedback loop on SERCA gene expression, Ca(2+) homeostasis, and insulin expression and secretion. Biochemistry. 2000 Dec 5;39(48):14912-9. doi: 10.1021/bi001260w. PubMed PMID: 11101307.

Gao Z, Reavey-Cantwell J, Young RA, Jegier P, Wolf BA. Synaptotagmin III/VII isoforms mediate Ca2+-induced insulin secretion in pancreatic islet beta -cells. J Biol Chem. 2000 Nov 17;275(46):36079-85. doi: 10.1074/jbc.M004284200. PubMed PMID: 10938083.

Talbot K, Young RA, Jolly-Tornetta C, Lee VM, Trojanowski JQ, Wolf BA. A frontal variant of Alzheimer's disease exhibits decreased calcium-independent phospholipase A2 activity in the prefrontal cortex. Neurochem Int. 2000 Jul;37(1):17-31. doi: 10.1016/s0197-0186(00)00006-1. PubMed PMID: 10781842.

Jolly-Tornetta C, Wolf BA. Regulation of amyloid precursor protein (APP) secretion by protein kinase calpha in human ntera 2 neurons (NT2N). Biochemistry. 2000 Jun 27;39(25):7428-35. doi: 10.1021/bi0003846. PubMed PMID: 10858291.

Coughlan CM, McManus CM, Sharron M, Gao Z, Murphy D, Jaffer S, Choe W, Chen W, Hesselgesser J, Gaylord H, Kalyuzhny A, Lee VM, Wolf B, Doms RW, Kolson DL. Expression of multiple functional chemokine receptors and monocyte chemoattractant protein-1 in human neurons. Neuroscience. 2000;97(3):591-600. doi: 10.1016/s0306-4522(00)00024-5. PubMed PMID: 10828541.

1999

Gao ZY, Li G, Najafi H, Wolf BA, Matschinsky FM. Glucose regulation of glutaminolysis and its role in insulin secretion. Diabetes. 1999 Aug;48(8):1535-42. doi: 10.2337/diabetes.48.8.1535. PubMed PMID: 10426370.

Major CD, Gao ZY, Wolf BA. Activation of the sphingomyelinase/ceramide signal transduction pathway in insulin-secreting beta-cells: role in cytokine-induced beta-cell death. Diabetes. 1999 Jul;48(7):1372-80. doi: 10.2337/diabetes.48.7.1372. PubMed PMID: 10389841.

Xu GG, Gao ZY, Borge PD Jr, Wolf BA. Insulin receptor substrate 1-induced inhibition of endoplasmic reticulum Ca2+ uptake in beta-cells. Autocrine regulation of intracellular ca2+ homeostasis and insulin secretion. J Biol Chem. 1999 Jun 18;274(25):18067-74. doi: 10.1074/jbc.274.25.18067. PubMed PMID: 10364259.

Young RA, Talbot K, Gao ZY, Trojanowski JQ, Wolf BA. Phospholipase pathway in Alzheimer's disease brains: decrease in Galphai in dorsolateral prefrontal cortex. Brain Res Mol Brain Res. 1999 Mar 20;66(1-2):188-90. doi: 10.1016/s0169-328x(99)00023-6. PubMed PMID: 10095092.

1998

Gao ZY, Chen M, Collins HW, Matschinsky FM, Lee VM, Wolf BA. Mechanisms of spontaneous cytosolic Ca2+ transients in differentiated human neuronal cells. Eur J Neurosci. 1998 Jul;10(7):2416-25. doi: 10.1046/j.1460-9568.1998.00252.x. PubMed PMID: 9749769.

Gao ZY, Xu G, Stwora-Wojczyk MM, Matschinsky FM, Lee VM, Wolf BA. Retinoic acid induction of calcium channel expression in human NT2N neurons. Biochem Biophys Res Commun. 1998 Jun 18;247(2):407-13. doi: 10.1006/bbrc.1998.8826. PubMed PMID: 9642141.

Jolly-Tornetta C, Gao ZY, Lee VM, Wolf BA. Regulation of amyloid precursor protein secretion by glutamate receptors in human Ntera 2 neurons. J Biol Chem. 1998 May 29;273(22):14015-21. doi: 10.1074/jbc.273.22.14015. PubMed PMID: 9593752.

Gao ZY, Collins HW, Matschinsky FM, Lee VM, Wolf BA. Cytotoxic effect of beta-amyloid on a human differentiated neuron is not mediated by cytoplasmic Ca2+ accumulation. J Neurochem. 1998 Apr;70(4):1394-400. doi: 10.1046/j.1471-4159.1998.70041394.x. PubMed PMID: 9523555.

Wolf BA. Assay of cellular diacylglycerol and monoacylglycerol lipases. Methods Mol Biol. 1998;105:167-74. doi: 10.1385/0-89603-491-7:167. Review. PubMed PMID: 10427560.

Eder AF, McGrath CM, Dowdy YG, Tomaszewski JE, Rosenberg FM, Wilson RB, Wolf BA, Shaw LM. Ethylene glycol poisoning: toxicokinetic and analytical factors affecting laboratory diagnosis. Clin Chem. 1998 Jan;44(1):168-77. PubMed PMID: 9550575.

1996

Konard RJ, Stoller JZ, Gao ZY, Wolf BA. Eicosapentaenoic acid (C20:5) augments glucose-induced insulin secretion from beta-TC3 insulinoma cells. Pancreas. 1996 Oct;13(3):253-8. doi: 10.1097/00006676-199610000-00007. PubMed PMID: 8884846.

Konrad RJ, Dean RM, Young RA, Billings PC, Wolf BA. Glucose-induced tyrosine phosphorylation of p125 in beta cells and pancreatic islets. A novel proximal signal in insulin secretion. J Biol Chem. 1996 Sep 27;271(39):24179-86. doi: 10.1074/jbc.271.39.24179. PubMed PMID: 8798659.

Eder AF, Dowdy YG, Gardiner JA, Wolf BA, Shaw LM. Serum lactate and lactate dehydrogenase in high concentrations interfere in enzymatic assay of ethylene glycol. Clin Chem. 1996 Sep;42(9):1489-91. PubMed PMID: 8787711.

Wolf BA. Overview of therapeutic drug monitoring and biotechnologic drugs. Ther Drug Monit. 1996 Aug;18(4):402-4. doi: 10.1097/00007691-199608000-00016. PubMed PMID: 8857559.

Chen M, Yang Z, Naji A, Wolf BA. Identification of calcium-dependent phospholipase A2 isoforms in human and rat pancreatic islets and insulin secreting beta-cell lines. Endocrinology. 1996 Jul;137(7):2901-9. doi: 10.1210/endo.137.7.8770912. PubMed PMID: 8770912.

Gao Z, Konrad RJ, Collins H, Matschinsky FM, Rothenberg PL, Wolf BA. Wortmannin inhibits insulin secretion in pancreatic islets and beta-TC3 cells independent of its inhibition of phosphatidylinositol 3-kinase. Diabetes. 1996 Jul;45(7):854-62. doi: 10.2337/diab.45.7.854. PubMed PMID: 8666133.

Harbeck MC, Louie DC, Howland J, Wolf BA, Rothenberg PL. Expression of insulin receptor mRNA and insulin receptor substrate 1 in pancreatic islet beta-cells. Diabetes. 1996 Jun;45(6):711-7. doi: 10.2337/diab.45.6.711. PubMed PMID: 8635642.

Bernard DW, Bowman RL, Grimm FA, Wolf BA, Simson MB, Shaw LM. Nighttime dosing assures post distribution sampling for therapeutic drug monitoring of digoxin. Clin Chem. 1996 Jan;42(1):45-9. PubMed PMID: 8565231.

1995

Rothenberg PL, Willison LD, Simon J, Wolf BA. Glucose-induced insulin receptor tyrosine phosphorylation in insulin-secreting beta-cells. Diabetes. 1995 Jul;44(7):802-9. doi: 10.2337/diab.44.7.802. PubMed PMID: 7540574.

Konrad RJ, Young RA, Record RD, Smith RM, Butkerait P, Manning D, Jarett L, Wolf BA. The heterotrimeric G-protein Gi is localized to the insulin secretory granules of beta-cells and is involved in insulin exocytosis. J Biol Chem. 1995 May 26;270(21):12869-76. doi: 10.1074/jbc.270.21.12869. PubMed PMID: 7759545.

Alter CA, Wolf BA. Identification of phosphatidylinositol 3,4,5-trisphosphate in pancreatic islets and insulin-secreting beta-cells. Biochem Biophys Res Commun. 1995 Mar 8;208(1):190-7. doi: 10.1006/bbrc.1995.1322. PubMed PMID: 7887929.

Wolf BA, Wertkin AM, Jolly YC, Yasuda RP, Wolfe BB, Konrad RJ, Manning D, Ravi S, Williamson JR, Lee VM. Muscarinic regulation of Alzheimer's disease amyloid precursor protein secretion and amyloid beta-protein production in human neuronal NT2N cells. J Biol Chem. 1995 Mar 3;270(9):4916-22. doi: 10.1074/jbc.270.9.4916. PubMed PMID: 7876266.

1994

Konrad RJ, Major CD, Wolf BA. Diacylglycerol hydrolysis to arachidonic acid is necessary for insulin secretion from isolated pancreatic islets: sequential actions of diacylglycerol and monoacylglycerol lipases. Biochemistry. 1994 Nov 15;33(45):13284-94. doi: 10.1021/bi00249a015. PubMed PMID: 7947736.

Frattali AL, Wolf BA. 1,5-Anhydroglucitol: a novel serum marker for screening and monitoring diabetes mellitus?. Clin Chem. 1994 Nov;40(11 Pt 1):1991-3. PubMed PMID: 7955366.

Major C, Wolf BA. Quantitation of the fatty acid composition of phosphatidic acid by capillary gas chromatography electron-capture detection with picomole sensitivity. J Chromatogr B Biomed Appl. 1994 Aug 19;658(2):233-40. doi: 10.1016/0378-4347(94)00254-1. PubMed PMID: 7820251.

Kelly CJ, Gallagher H, Wolf BA, Daly JM. Alterations in macrophage signal transduction pathways mediate post-traumatic changes in macrophage function. J Surg Res. 1994 Jul;57(1):221-6. doi: 10.1006/jsre.1994.1135. PubMed PMID: 8041143.

Alter CA, Amagasu M, Shah K, Jolly YC, Major C, Wolf BA. U-73122 does not specifically inhibit phospholipase C in rat pancreatic islets and insulin-secreting beta-cell lines. Life Sci. 1994;54(8):PL107-12. doi: 10.1016/0024-3205(94)90009-4. PubMed PMID: 8107526.

1993

Baffy G, Yang L, Wolf BA, Williamson JR. G-protein specificity in signaling pathways that mobilize calcium in insulin-secreting beta-TC3 cells. Diabetes. 1993 Dec;42(12):1878-82. doi: 10.2337/diab.42.12.1878. PubMed PMID: 8243834.

Jolly YC, Major C, Wolf BA. Transient activation of calcium-dependent phospholipase A2 by insulin secretagogues in isolated pancreatic islets. Biochemistry. 1993 Nov 16;32(45):12209-17. doi: 10.1021/bi00096a034. PubMed PMID: 8218298.

Konrad RJ, Jolly YC, Major C, Wolf BA. Fuel secretagogue stimulation of arachidonic acid accumulation in fresh and cultured pancreatic islets. Mol Cell Endocrinol. 1993 Mar;92(1):135-40. doi: 10.1016/0303-7207(93)90084-w. PubMed PMID: 8472864.

1992

Krause DS, Wolf BA, Shaw LM. Acute aspirin overdose: mechanisms of toxicity. Ther Drug Monit. 1992 Dec;14(6):441-51. doi: 10.1097/00007691-199212000-00001. Review. PubMed PMID: 1485363.

Corbett JA, Wang JL, Hughes JH, Wolf BA, Sweetland MA, Lancaster JR Jr, McDaniel ML. Nitric oxide and cyclic GMP formation induced by interleukin 1 beta in islets of Langerhans. Evidence for an effector role of nitric oxide in islet dysfunction. Biochem J. 1992 Oct 1;287 ( Pt 1):229-35. doi: 10.1042/bj2870229. PubMed PMID: 1384465; PubMed Central PMCID: PMC1133148.

Konrad RJ, Jolly YC, Major C, Wolf BA. Carbachol stimulation of phospholipase A2 and insulin secretion in pancreatic islets. Biochem J. 1992 Oct 1;287 ( Pt 1):283-90. doi: 10.1042/bj2870283. PubMed PMID: 1417779; PubMed Central PMCID: PMC1133156.

Konrad RJ, Jolly YC, Major C, Wolf BA. Inhibition of phospholipase A2 and insulin secretion in pancreatic islets. Biochim Biophys Acta. 1992 Jun 10;1135(2):215-20. doi: 10.1016/0167-4889(92)90139-3. PubMed PMID: 1616940.

Landt M, Easom RA, Colca JR, Wolf BA, Turk J, Mills LA, McDaniel ML. Parallel effects of arachidonic acid on insulin secretion, calmodulin-dependent protein kinase activity and protein kinase C activity in pancreatic islets. Cell Calcium. 1992 Mar;13(3):163-72. doi: 10.1016/0143-4160(92)90044-s. PubMed PMID: 1315620.

1991

Konrad RJ, Jolly YC, Wolf BA. Glucose and carbachol synergistically stimulate phosphatidic acid accumulation in pancreatic islets. Biochem Biophys Res Commun. 1991 Oct 31;180(2):960-6. doi: 10.1016/s0006-291x(05)81159-3. PubMed PMID: 1953765.

Wolf BA, Pasquale SM, Turk J. Free fatty acid accumulation in secretagogue-stimulated pancreatic islets and effects of arachidonate on depolarization-induced insulin secretion. Biochemistry. 1991 Jul 2;30(26):6372-9. doi: 10.1021/bi00240a004. PubMed PMID: 1905151.

Wolf BA, Williamson JR, Easom RA, Chang K, Sherman WR, Turk J. Diacylglycerol accumulation and microvascular abnormalities induced by elevated glucose levels. J Clin Invest. 1991 Jan;87(1):31-8. doi: 10.1172/JCI114988. PubMed PMID: 1985103; PubMed Central PMCID: PMC294984.

1990

Wolf BA, Conrad-Kessel W, Turk J. Long-chain fatty alcohol quantitation in subfemtomole amounts by gas chromatography-negative ion chemical ionization mass spectrometry. Application to long-chain acyl coenzyme A measurement. J Chromatogr. 1990 Jun 22;509(2):325-32. doi: 10.1016/s0021-9673(01)93090-0. PubMed PMID: 2211898.

Wolf BA, Easom RA, McDaniel ML, Turk J. Diacylglycerol synthesis de novo from glucose by pancreatic islets isolated from rats and humans. J Clin Invest. 1990 Feb;85(2):482-90. doi: 10.1172/JCI114463. PubMed PMID: 2405021; PubMed Central PMCID: PMC296449.

Elson EL, Pasternak C, Liu ZY, Young JI, Schwab B 3rd, Worthen GS, Downey G, Michaels R, McConnaughey WB, McDaniel M. Activation of mechanical responses in leukocytes. Biorheology. 1990;27(6):849-58. doi: 10.3233/bir-1990-27605. PubMed PMID: 2093393.

Turk J, Stump WT, Conrad-Kessel W, Seabold RR, Wolf BA. Quantitation of epoxy- and dihydroxyeicosatrienoic acids by stable isotope-dilution mass spectrometry. Methods Enzymol. 1990;187:175-86. doi: 10.1016/0076-6879(90)87023-v. PubMed PMID: 2233344.

1989

Easom RA, Hughes JH, Landt M, Wolf BA, Turk J, McDaniel ML. Comparison of effects of phorbol esters and glucose on protein kinase C activation and insulin secretion in pancreatic islets. Biochem J. 1989 Nov 15;264(1):27-33. doi: 10.1042/bj2640027. PubMed PMID: 2690823; PubMed Central PMCID: PMC1133543.

Hughes JH, Easom RA, Wolf BA, Turk J, McDaniel ML. Interleukin 1-induced prostaglandin E2 accumulation by isolated pancreatic islets. Diabetes. 1989 Oct;38(10):1251-7. doi: 10.2337/diab.38.10.1251. PubMed PMID: 2507377.

Wolf BA, Garrett NC, Nahm MH. The "hook effect": high concentrations of prostate-specific antigen giving artifactually low values on one-step immunoassay. N Engl J Med. 1989 Jun 29;320(26):1755-6. doi: 10.1056/NEJM198906293202614. PubMed PMID: 2471931.

Bodor G, Wolf BA, Hinds B, Nahm MH, Scott MG. More on "hook effects" in immunometric assays for prostate-specific antigen. Clin Chem. 1989 Jun;35(6):1262-3. PubMed PMID: 2471604.

Wolf BA, Easom RA, Hughes JH, McDaniel ML, Turk J. Secretagogue-induced diacylglycerol accumulation in isolated pancreatic islets. Mass spectrometric characterization of the fatty acyl content indicates multiple mechanisms of generation. Biochemistry. 1989 May 16;28(10):4291-301. doi: 10.1021/bi00436a026. PubMed PMID: 2504278.

Wolf BA, Hughes JH, Florholmen J, Turk J, McDaniel ML. Interleukin-1 inhibits glucose-induced Ca2+ uptake by islets of Langerhans. FEBS Lett. 1989 May 8;248(1-2):35-8. doi: 10.1016/0014-5793(89)80426-0. PubMed PMID: 2656294.

Turk J, Wolf BA, Easom RA, Hughes JH, McDaniel ML. Arachidonic acid metabolism in isolated pancreatic islets. V. The enantiomeric composition of 12-hydroxy-5,8,10,14-eicosatetraenoic acid indicates synthesis by a 12-lipoxygenase rather than a monooxygenase. Biochim Biophys Acta. 1989 Jan 23;1001(1):16-24. doi: 10.1016/0005-2760(89)90301-9. PubMed PMID: 2492199.

Wolf BA, Daft MC, Koenig JW, Flye MW, Turk JW, Scott MG. Measurement of cyclosporine concentrations in whole blood: HPLC and radioimmunoassay with a specific monoclonal antibody and 3H- or 125I-labeled ligand compared. Clin Chem. 1989 Jan;35(1):120-4. PubMed PMID: 2642760.

1988

Turk J, Stump WT, Wolf BA, Easom RA, McDaniel ML. Quantitative stereochemical analysis of subnanogram amounts of 12-hydroxy-(5,8,10,14)-eicosatetraenoic acid by sequential chiral phase liquid chromatography and stable isotope dilution mass spectrometry. Anal Biochem. 1988 Nov 1;174(2):580-8. doi: 10.1016/0003-2697(88)90059-0. PubMed PMID: 3071179.

Vaidya HC, Wolf BA, Garrett N, Catalona WJ, Clayman RV, Nahm MH. Extremely high values of prostate-specific antigen in patients with adenocarcinoma of the prostate; demonstration of the "hook effect". Clin Chem. 1988 Oct;34(10):2175-7. PubMed PMID: 2458866.

McDaniel ML, Hughes JH, Wolf BA, Easom RA, Turk JW. Descriptive and mechanistic considerations of interleukin 1 and insulin secretion. Diabetes. 1988 Oct;37(10):1311-5. doi: 10.2337/diab.37.10.1311. PubMed PMID: 3046964.

Turk J, Hughes JH, Easom RA, Wolf BA, Scharp DW, Lacy PE, McDaniel ML. Arachidonic acid metabolism and insulin secretion by isolated human pancreatic islets. Diabetes. 1988 Jul;37(7):992-6. doi: 10.2337/diab.37.7.992. PubMed PMID: 3133262.

Wolf BA, Florholmen J, Turk J, McDaniel ML. Studies of the Ca2+ requirements for glucose- and carbachol-induced augmentation of inositol trisphosphate and inositol tetrakisphosphate accumulation in digitonin-permeabilized islets. Evidence for a glucose recognition site in insulin secretion. J Biol Chem. 1988 Mar 15;263(8):3565-75. PubMed PMID: 2831191.

Wolf BA, Colca JR, Turk J, Florholmen J, McDaniel ML. Regulation of Ca2+ homeostasis by islet endoplasmic reticulum and its role in insulin secretion. Am J Physiol. 1988 Feb;254(2 Pt 1):E121-36. doi: 10.1152/ajpendo.1988.254.2.E121. Review. PubMed PMID: 3279798.

Colca JR, Wolf BA, McDaniel ML. Regulation of calcium uptake/efflux from the islet-cell endoplasmic reticulum with regard to the secretion of insulin. Prog Clin Biol Res. 1988;265:117-32. PubMed PMID: 3043440.

McDaniel ML, Wolf BA, Turk J. Signal transduction and insulin secretion: role of intracellular Ca2+, inositoltrisphosphate, and arachidonic acid. Prog Clin Biol Res. 1988;265:99-116. PubMed PMID: 3136457.

1987

Comens PG, Wolf BA, Unanue ER, Lacy PE, McDaniel ML. Interleukin 1 is potent modulator of insulin secretion from isolated rat islets of Langerhans. Diabetes. 1987 Aug;36(8):963-70. doi: 10.2337/diab.36.8.963. PubMed PMID: 3297891.

Hugues JN, Wolf B, Sebaoun J, Buisson N, Gourdji D. Is thyrotropin-releasing hormone receptor involved in thyrotrope adaptation to starvation?. Acta Endocrinol (Copenh). 1987 Jul;115(3):353-6. doi: 10.1530/acta.0.1150353. PubMed PMID: 3039768.

Leduque P, Aratan-Spire S, Wolf B, Dubois PM, Czernichow P. Localization of thyrotropin-releasing hormone- and insulin-immunoreactivity in the pancreas of neonatal rats after injection of streptozotocin at birth. Cell Tissue Res. 1987 Apr;248(1):89-94. doi: 10.1007/bf01239967. PubMed PMID: 2952279.

Wolf BA, Florholmen J, Colca JR, McDaniel ML. GTP mobilization of Ca2+ from the endoplasmic reticulum of islets. Comparison with myo-inositol 1,4,5-trisphosphate. Biochem J. 1987 Feb 15;242(1):137-41. doi: 10.1042/bj2420137. PubMed PMID: 3297043; PubMed Central PMCID: PMC1147675.

Turk J, Wolf BA, McDaniel ML. The role of phospholipid-derived mediators including arachidonic acid, its metabolites, and inositoltrisphosphate and of intracellular Ca2+ in glucose-induced insulin secretion by pancreatic islets. Prog Lipid Res. 1987;26(2):125-81. doi: 10.1016/0163-7827(87)90010-5. Review. PubMed PMID: 3116559.

1986

Wolf BA, Colca JR, McDaniel ML. Calmodulin inhibits inositol trisphosphate-induced Ca2+ mobilization from the endoplasmic reticulum of islets. Biochem Biophys Res Commun. 1986 Dec 15;141(2):418-25. doi: 10.1016/s0006-291x(86)80189-9. PubMed PMID: 2948504.

Wolf BA, Colca JR, Comens PG, Turk J, McDaniel ML. Glucose 6-phosphate regulates Ca2+ steady state in endoplasmic reticulum of islets. A possible link in glucose-induced insulin secretion. J Biol Chem. 1986 Dec 15;261(35):16284-7. PubMed PMID: 3023346.

Turk J, Wolf BA, Lefkowith JB, Stump WT, McDaniel ML. Glucose-induced phospholipid hydrolysis in isolated pancreatic islets: quantitative effects on the phospholipid content of arachidonate and other fatty acids. Biochim Biophys Acta. 1986 Dec 5;879(3):399-409. doi: 10.1016/0005-2760(86)90232-8. PubMed PMID: 3535899.

Turk J, Wolf BA, McDaniel ML. Glucose-induced accumulation of inositol triphosphates in isolated pancreatic islets. Predominance of the 1,3,4-isomer. Biochem J. 1986 Jul 1;237(1):259-63. doi: 10.1042/bj2370259. PubMed PMID: 3541896; PubMed Central PMCID: PMC1146973.

Turk J, Wolf BA, McDaniel ML. Quantitation of myo-inositol as its hexakis(trifluoroacetyl) derivative with negative ion chemical ionization mass spectrometry. Biomed Environ Mass Spectrom. 1986 May;13(5):237-44. doi: 10.1002/bms.1200130506. PubMed PMID: 2941090.

Bullier-Picard F, Wolf BA, Hugues JN, Durand D, Voirol MJ, Charrier J, Czernichow P, Postel-Vinay MC. The Brattleboro rat: normal growth hormone secretion, decreased hepatic growth hormone receptors and low plasma somatomedin activity. Mol Cell Endocrinol. 1986 Apr;45(1):49-56. doi: 10.1016/0303-7207(86)90081-x. PubMed PMID: 3009248.

Wolf BA, Turk J, Sherman WR, McDaniel ML. Intracellular Ca2+ mobilization by arachidonic acid. Comparison with myo-inositol 1,4,5-trisphosphate in isolated pancreatic islets. J Biol Chem. 1986 Mar 15;261(8):3501-11. PubMed PMID: 3081507.

Aratan-Spire S, Wolf B, Czernichow P. Enzymatic degradation of thyrotropin releasing hormone by pancreatic homogenates. Failure to detect His-Pro-diketopiperazine as TRH metabolite. Neuroendocrinology. 1986;42(5):399-406. doi: 10.1159/000124478. PubMed PMID: 3084986.

1985

Colca JR, Wolf BA, Comens PG, McDaniel ML. Protein phosphorylation in permeabilized pancreatic islet cells. Biochem J. 1985 Jun 15;228(3):529-36. doi: 10.1042/bj2280529. PubMed PMID: 3896230; PubMed Central PMCID: PMC1145019.

Turk J, Wolf BA, Comens PG, Colca J, Jakschik B, McDaniel ML. Arachidonic acid metabolism in isolated pancreatic islets. IV. Negative ion-mass spectrometric quantitation of monooxygenase product synthesis by liver and islets. Biochim Biophys Acta. 1985 Jun 14;835(1):1-17. doi: 10.1016/0005-2760(85)90023-2. PubMed PMID: 3924104.

Wolf BA, Hugues JN, Aratan-Spire S, Reinberg A, Voirol MJ, Czernichow P. Hypothalamo-pituitary regulation of thyrotrophin secretion in chronically catheterized Brattleboro rats. J Endocrinol. 1985 May;105(2):277-83. doi: 10.1677/joe.0.1050277. PubMed PMID: 3921647.

Wolf BA, Comens PG, Ackermann KE, Sherman WR, McDaniel ML. The digitonin-permeabilized pancreatic islet model. Effect of myo-inositol 1,4,5-trisphosphate on Ca2+ mobilization. Biochem J. 1985 May 1;227(3):965-9. doi: 10.1042/bj2270965. PubMed PMID: 3890834; PubMed Central PMCID: PMC1144928.

Leduque P, Wolf B, Aratan-Spire S, Dubois PM, Czernichow P. Immunocytochemical location of thyrotropin-releasing hormone (TRH) in the B-cells of adult hypothyroid rat pancreas. Regul Pept. 1985 Apr;10(4):281-92. doi: 10.1016/0167-0115(85)90040-0. PubMed PMID: 3923574.

1984

Czernichow P, Wolf B, Fermanian J, Pomarede R, Rappaport R. Twenty-four hour variations of thyroid hormones and thyrotrophin concentrations in hypothyroid infants treated with L-thyroxine. Clin Endocrinol (Oxf). 1984 Oct;21(4):393-7. doi: 10.1111/j.1365-2265.1984.tb03226.x. PubMed PMID: 6509783.

Aratan-Spire S, Wolf B, Czernichow P. Effects of hypo- and hyperthyroidism on pancreatic TRH-degrading activity and TRH concentrations in developing rat pancreas. Acta Endocrinol (Copenh). 1984 Jun;106(2):209-14. doi: 10.1530/acta.0.1060209. PubMed PMID: 6428122.

Aratan-Spire S, Wolf B, Portha B, Bailbe D, Czernichow P. Streptozotocin treatment at birth induces a parallel depletion of thyrotropin-releasing hormone and insulin in the rat pancreas during development. Endocrinology. 1984 Jun;114(6):2369-73. doi: 10.1210/endo-114-6-2369. PubMed PMID: 6233134.

Aratan-Spire S, Wolf B, Czernichow P. Developmental pattern of TRH-degrading activity and TRH content in rat pancreas. Acta Endocrinol (Copenh). 1984 May;106(1):102-8. doi: 10.1530/acta.0.1060102. PubMed PMID: 6428113.

Wolf B, Aratan-Spire S, Czernichow P. Hypothyroidism increases pancreatic thyrotropin-releasing hormone concentrations in adult rats. Endocrinology. 1984 Apr;114(4):1334-7. doi: 10.1210/endo-114-4-1334. PubMed PMID: 6423372.

Savino W, Wolf B, Aratan-Spire S, Dardenne M. Thymic hormone containing cells. IV. Fluctuations in the thyroid hormone levels in vivo can modulate the secretion of thymulin by the epithelial cells of young mouse thymus. Clin Exp Immunol. 1984 Mar;55(3):629-35. PubMed PMID: 6584256; PubMed Central PMCID: PMC1535945.

1983

Wolf B, Gaultier C, Lopez C, Boulé M, Girard F. Hypoxemia in attack free asthmatic children: relationship with lung volumes and lung mechanics. Bull Eur Physiopathol Respir. 1983 Sep-Oct;19(5):471-6. PubMed PMID: 6640166.

Awards and Honors

2015, Computerworld Premier 100 IT Leaders
1994-1998, Research and Career Development Award, National Institutes of Health
1993-1994, Hartford Foundation Fellow
1992, Award for Outstanding Scientific Achievements by a Young Investigator, American Association of Clinical Chemistry
1990-1993, Research and Development Award, American Diabetes Association
1990, Career Development Award, Juvenile Diabetes Foundation
1989, Young Investigator Award, Academy of Clinical Laboratory Physicians and Scientists
1986-1987, Juvenile Diabetes Foundation Fellowship
1986, Endocrine Society Travel Award
1992, Diplomate, American Board of Pathology in clinical pathology

Leadership and Memberships

Memberships in Professional Organizations

International

1991-present, International Association of Therapeutic Drug Monitoring and Clinical Toxicology
1990-present, European Association for the Study of Diabetes

National

2009-present, College of Healthcare Information Management Executives
2003-present, College of American Pathologists
2001-present, American Society for Investigative Pathology
2001-present, American Society for Clinical Investigation
1991-present, Academy of Clinical Laboratory Physicians and Scientists
1989-present, American Association for Clinical Chemistry Inc.
1986-present, American Society for Biochemistry and Molecular Biology Inc.
1985-present, American Diabetes Association
1985-present, American Association for the Advancement of Science