Haralambos Ischiropoulos, PhD

Locations: Main Campus
Appointments and Referrals: 1-800-TRY-CHOP

  • Background

    Our laboratory investigates the biological chemistry and signaling pathways of nitric oxide by using biochemical and molecular methodologies in partnership with mass spectroscopy-based approaches. We uncovered that selective modification of cysteine residue by nitric oxide to generate S-nitrosocysteine regulates the function of enzymes participating in metabolism and mitochondrial bioenergetics. Current efforts are centered on uncovering the importance of S-nitrosocysteine in the regulation of B-oxidation of fatty acids and in the coordination of liver responses to metabolic deprivation or overloading. Another project investigates the utility of pharmacological agents that restore nitric oxide signaling to treat long-chain fatty acid oxidation disorders.

    Weare also generating inventories of cellular proteomes and secretomes of the mouse brain. These inventories are used to create 3D-functional landscapes of the mouse brain extracellular space and to identify astrocyte secreted proteins that influence neuronal development and neuronal demise. Other studies explore the role of nitric oxide and nitric-oxide mediated protein S-nitrosylation in the neuronastrocyte metabolism of glutamate.

  • Education and Training

    Graduate Degree

    Experimental Pathology - New York Medical College, Valhalla, NY

  • Titles and Academic Titles

    Physician

    Research Professor of Pediatrics and Pharmacology, Perelman School of Medicine at the University of Pennsylvania

  • Centers and Programs
  • Publications

    Papers

    2013

    Doulias P-T. Tenopoulou M, Greene, JL, Raju, K and Ischiropoulos H. Nitric oxide regulates mitochondrial fatty acid metabolism through reversible protein S-nitrosylation. Sci. Signal. 6:rs1-7,2013.

    2012

    Martinez M, Cuker A, Mills A, Lightfoot R, Fan Y, Tang "VB, Hazen SL, Ischiropoulos H. Nitrated fibrinogen is a biomarker of oxidative stress in venous thromboembolism. Free Radic Biol Med. 53:230-236,2012. PMC3392413.

    Malleus K and Ischiropoulos H, Regional deficiencies in chaperone-mediated autophagy underlie a-synuclein aggregation and neurodegeneration. Neurobiol. Dis. 46:732-744,2012. PMC335297. 

    Thomson L, Tenopoulou M, Lightfoot R, Tsika E, Parastatidis I, Martinez M, Greco TM; Doulias P-T, Wu Y, Tang WHW, Hazen SL, Ischiropoulos H. Immunoglobulins against tyrosine nitrated epitopes in coronary artery disease. Circulation 126:2392-2401, 2012. PMC3501999.

  • Editorial and Academic Positions

    Editorial positions

    2009-2013, Editorial board, Journal of Biological Chemistry
    2009-present, Associate editor, Free Radical Biology and Medicine
    2005-2009, Editorial Advisory Panel, Biochemical Journal
    2005-2009, Current Respiratory Medicine Reviews
    2003-present, Faculty Chemical Biology Section, Faculty 1000

    Academic committees

    2009-2013, Research Institute Faculty Advisory Committee on Compliance, The Children's Hospital of Philadelphia
    2007-present, Deputy Chair, Research Institute-Cores Committee, The Children's Hospital of Philadelphia
    2006-present, Director of Neonatology Research, The Children's Hospital of Philadelphia Research Institute