Blood Bank Clinical Rotation Schedule – MLS Students
Week 1
- Introduction and tour of the Blood Bank
- Processing blood and blood products from American Red Cross
- Confirming ABO/Rh type of whole blood and red cells
- Introduction to Laboratory Information System
- Reading and grading reactions
- Entering and processing FFP, cryo, random donor platelets and single donor platelets
- Temperature requirements/restrictions for transportation and storage of blood products
- Hemoglobin S testing and antigen typed units from blood supplier
- Introduction to gel technology
Week 2
- Specimen acceptability
- Perform Type and Screens and crossmatches on real patients using gel technology
- History check requirements including safety checks as well as accreditation requirements
- DATs in tube and IgG DATs in gel (explain implications and mechanisms of positive DAT
- DATs in tube and IgG DATs in gel (explain implications and mechanisms of positive DAT)
- Antigen testing on both, patients and donors (include the effects of a positive DAT on antigen typing)
- Perform testing on expired samples for practice in both, gel and tube technology
- Mid-term evaluation
Week 3
- Perform antibody Screens on patients with known positives
- Discuss importance of history check in antibody identification
- Perform Antibody Identification
- Panel selection in Antigen Plus System
- Dosage effect
- Single and multiple antibodies
- Use of enzymes in antibody identification
- 3+ / 3- rule and statistical validity
- Previously identified antibodies
- Given expired samples with known antibodies
- Students given known samples as wet practicum
- Mixed field reactions
- Neonatal type and screens
- Fetal-maternal incompatibility and selection of blood products for neonates
- Rhogam (why it is given prophylactically)
- Eluate: Acid eluate and Lui Freeze Thaw elute preparation
- Warm auto antibody and cold auto antibody and how this affects patient transfusion
Week 4
- Introduction to component preparation
- Irradiation of blood and platelets
- Why we irradiate
- Which products are irradiated
- CHOP irradiation policy
- Washing a unit of red blood cells using COBE cell washers
- Why we wash
- Expiration time
- Platelet selection
- Saline suspension of platelets
- Thawing plasma and cryoprecipitate
- Expiration time
- Preparing aliquots using syringe
- Labeling requirements
- Expiration times
- Adsol Reduction of red blood cells
- Tagging of unit of blood or blood products for patients
- Communication with clinical staff (phone etiquette)
- Reagent QC (watch/perform)
- Equipment QC (watch/perform)
- Final wet practicum, written quiz and final evaluation