Education and Training
PhD - Hebrew University of Jerusalem (Pharmaceutical Science), Jerusalem, Israel
Medicine - Central Hospital #6, Penza, Russia
Postdoctoral Fellow, Division of Cardiology, Children's Hospital of Philadelphia
MD - University of Saratov; Saratov, Russia
Titles and Academic Titles
Research Associate Professor of Pediatrics, Perelman School of Medicine at the University of Pennsylvania
Departments and Services
- Gene and cell therapy of cardiovascular diseases
- Role of macrophages in the pathogenesis of in-stent restenosis
- Surface modification of biomaterials for improving biocompatibility of medical implants
Inamdar VV, Fitzpatrick EG, Alferiev IS, Levy RJ, Stachelek SJ, Fishbein I: Mitigation of blood borne cell attachment to metal implants through CD47-derived peptide immobilization. Journal of Visualized Experiments(166), Dec 3 2020 Notes: doi: 10.3791/61545.
Inamdar Vaishali V, Fitzpatrick Emmett, Alferiev Ivan, Nagaswami Chandrasekaran, Spruce Lynn A, Fazelinia Hossein, Bratinov George, Seeholzer Steven H, Levy Robert J, Fishbein Ilia, Stachelek Stanley J: Stability and bioactivity of pepCD47 attachment on stainless steel surfaces. Acta biomaterialia 104: 231-240, Mar 2020 Notes: NIHMS ID 1583105.
Battig MR, Alferiev IS, Guerrero DT, Fishbein I, Pressly BB, Levy RJ, Chorny M: An Experimental Single-Platform Approach to Enhance Functionalization of Magnetically Targetable Cells. ACS Applied Bio Materials 2020 Notes: accepted; mt-2020-00466a.R1; DOI: 10.1021/acsabm.0c00466; NIHMS1597029.
Battig Mark R, Fishbein Ilia, Levy Robert J, Alferiev Ivan S, Guerrero David, Chorny Michael: Optimizing endothelial cell functionalization for cell therapy of vascular proliferative disease using a direct contact co-culture system. Drug delivery and translational research 8(4): 954-963, Aug 2018.
Fishbein I, Guerrero DT, Alferiev IS, Foster JB, Minutolo NG, Chorny M, Monteys AM, Driesbaugh KH, Nagaswami C, Levy RJ: Stent-based delivery of adeno-associated viral vectors with sustained vascular transduction and iNOS-mediated inhibition of in-stent restenosis. Gene Ther 24(11): 717-726, Nov 2017.
Fishbein I, WelchT ,Guerrero DT ,Alferiev IS ,Adamo RF , Chorny M, Gupte RK, Tang Y, Levy RJ: Paraffin Processing of Stented Arteries Using a Post-Fixation Dissolution of Metallic and Polymeric Stents. Cardiovascular Pathology. 2016 Aug 20;25(6):483-488. PMID: 27616613 (eds.). 25(6): 483-488, Aug 2016.
Slee JB, Alferiev IS, Nagaswami C, Weisel JW, Levy RJ, Fishbein I, Stachelek SJ: Enhanced biocompatibility of CD47-functionalized vascular stents. Biomaterials 87: 82-92, Feb 2016 Notes: joint senior author. PMCID: NIHMS762654
Adamo RF, Fishbein I, Zhang K, Wen J, Levy RJ, Alferiev IS, Chorny M.: Magnetically enhanced cell delivery for accelerating recovery of the endothelium in injured arteries. Journal of Controlled Release 222: 169-175, Jan 2016 Notes: joint first author. PMCID: PMC4718792
Alferiev IS, Iyer R, Croucher JL, Adamo RF, Zhang K, Mangino JL, Kolla V, Fishbein I, Brodeur GM, Levy RJ, Chorny M: Nanoparticle-mediated delivery of a rapidly activatable prodrug of SN-38 for neuroblastoma therapy. Biomaterials 51(1): 22-29, May 2015.
Tengood JE, Alferiev IS, Zhang K, Fishbein I, Levy RJ, Chorny M: Real-time analysis of composite magnetic nanoparticle disassembly in vascular cells and biomimetic media. PNAS 111(11): 4245-4250, Mar 2014.
Fishbein I, Forbes SP, Adamo RF, Chorny M, Levy RJ, Alferiev IS: Vascular gene transfer from metallic stent surfaces using adenoviral vectors tethered through hydrolysable cross-linkers. Journal of Visualized Experiments(90), e51653, Aug 2014 Notes: doi:10.3791/51653; http://www.jove.com/video/51653.
Kruklitis RJ, Fishbein I, Singhal S, Kappor V, Levy RJ, Alferiev IS, Sterman DH: Stent-mediated gene delivery for site-specific transgene administration to the airway epithelium and management of tracheobronchial tumors. Respiration 88(5): 406-417, Nov 2014 Notes: joint first authorship.
Ma G, Wang Y, Fishbein I, Yu M, Zhang L, Alferiev IS, Yang J, Song C, Levy RJ: Anchoring of self-assembled plasmid DNA/anti-DNA antibody/cationic lipid micelles on bisphosphonate-modified stent for cardiovascular gene delivery. International J Nanomedicine 8: 1029-35, Mar 2013.
Chorny M, Fishbein I, Tengood JE, Adamo RF, Alferiev IS, Levy RJ: Site-specific gene delivery to stented arteries using magnetically guided zinc oleate-based nanoparticles loaded with adenoviral vectors. FASEB journal 27(6): 2198-206, Jun 2013.
Fishbein I, Forbes SP, Chorny M, Connolly JM, Adamo RF, Corrales RA, Alferiev IS, Levy RJ: Adenoviral vector tethering to metal surfaces via hydrolyzable cross-linkers for the modulation of vector release and transduction. Biomaterials 34(28): 6938-48, Sep 2013.
Forbes SP, Alferiev IS, Chorny M, Adamo RF, Levy RJ, Fishbein I: Modulation of NO and ROS production by AdiNOS transduced vascular cells through supplementation with L-Arg and BH4: Implications for gene therapy of restenosis. Atherosclerosis 230(1): 23-32, Sep 2013.
Chorny M, Alferiev IS, Fishbein I, Tengood JE, Folchman-Wagner Z, Forbes SP, Levy RJ: Formulation and in vitro characterization of composite biodegradable magnetic nanoparticles for magnetically guided cell delivery. Pharmaceutical research 29(5): 1232-41, May 2012.
Chorny M, Fishbein I, Yellen BB, Alferiev IS, Bakay M, Ganta S, Adamo RF, Amiji M, Friedman G, Levy RJ: Targeting stents with local delivery of paclitaxel-loaded magnetic nanoparticles using uniform fields. PNAS (Proceedings of the National Academy of Sciences of the United States of America) 107(18): 8346-51, May 2010 Notes: joint first authorship.
Chorny M, Fishbein I, Alferiev IS, Levy RJ: Magnetically responsive biodegradable nanoparticles enhance adenoviral gene transfer in cultured smooth muscle and endothelial cells. Molecular pharmaceutics 6(5): 1380-7, Sep-Oct 2009.
Polyak B, Fishbein I, Chorny M, Alferiev IS, Williams D, Yellen BB, Friedman G, Levy RJ: High field gradient targeting of magnetic nanoparticle-loaded endothelial cells to the surfaces of steel stents. PNAS (Proceedings of the National Academy of Sciences of the United States of America) 105(2): 698-703, Jan 2008 Notes: joint first authorship.
Fishbein I, Alferiev IS, Bakay M, Stachelek SJ, Sobolewski P, Lai M, Choi H, Chen I-W, Levy RJ: Local delivery of gene vectors from bare-metal stents by use of a biodegradable synthetic complex inhibits in-stent restenosis in rat carotid arteries. Circulation 117(16): 2096-103, Apr 2008.
Fishbein I, Alferiev IS, Nyanguile O, Gaster R, Vohs JM, Wong GS, Felderman H, Chen I-W, Choi H, Wilensky RL, Levy RJ: Bisphosphonate-mediated gene vector delivery from the metal surfaces of stents. PNAS (Proceedings of the National Academy of Sciences of the United States of America) 103(1): 159-64, Jan 2006.
Chorny M, Fishbein I, Alferiev IS, Nyanguile O, Gaster R, Levy RJ: Adenoviral gene vector tethering to nanoparticle surfaces results in receptor-independent cell entry and increased transgene expression. Molecular therapy 14(3): 382-91, Sep 2006 Notes: joint first authorship.
Banai S, Chorny M, Gertz SD, Fishbein I, Gao J, Perez L, Lazarovichi G, Gazit A, Levitzki A, Golomb G.: Locally delivered nanoencapsulated tyrphostin (AGL-2043) reduces neointima formation in balloon-injured rat carotid and stented porcine coronary arteries. Biomaterials 26(4): 451-461, Feb 2005.
Burton DY, Song C, Fishbein I, Hazelwood S, Li Q, DeFelice S, Connolly JM, Perlstein I, Coulter DA, Levy RJ.: The incorporation of an ion channel gene mutation associated with the long QT syndrome (Q9E-hMiRP1) in a plasmid vector for site-specific arrhythmia gene therapy: in vitro and in vivo feasibility studies. Hum Gene Ther 14(9): 907-922, Jun 2003.
Danenberg HD, Fishbein I, Epstein H, Waltenberger J, Moerman E, Monkkonen J, Gao J, Gathi I, Reichi R, Golomb G.: Systemic depletion of macrophages by liposomal bisphosphonates reduces neointimal formation following balloon-injury in the rat carotid artery. J Cardiovasc Pharmacol 42(5): 671-679, Nov 2003.
Danenberg HD, Fishbein I, Gao J, Monkkonen J, Reich R, Gati I, Moerman E, Golomb G.: Macrophage depletion by clodronate-containing liposomes reduces neointimal formation after balloon injury in rats and rabbits. Circulation 106(5): 599-605, Jul 2002.
Margolin L, Fishbein I, Banai S, Golomb G, Reich R, Perez LS, Gertz SD.: Metalloproteinase inhibitor attenuates neointima formation and constrictive remodeling after angioplasty in rats: augmentative effect of alpha(v)beta(3) receptor blockade. Atherosclerosis 163(2): 269-277, Aug 2002.
Chorny M, Fishbein I, Danenberg HD, Golomb G.: Lipophilic drug loaded nanospheres prepared by nanoprecipitation: effect of formulation variables on size, drug recovery and release kinetics. J Control Release 83(3): 389-400, Oct 2002.
Chorny M, Fishbein I, Danenberg HD, Golomb G.: Study of the drug release mechanism from tyrphostin AG-1295-loaded nanospheres by in situ and external sink methods. J Control Release 83(3): 401-414, Oct 2002.
Karck M, Meliss R, Hestermann M, Mengel M, Pethig K, Levitzki A, Banai S, Golomb G, Fishbein I, Chorny M, Haverich A.: Inhibition of aortic allograft vasculopathy by local delivery of platelet-derived growth factor receptor tyrosine-kinase blocker AG-1295. Transplantation 74(9): 1335-1341, Nov 2002.
Alferiev IS, Fishbein I.: Activated polyurethane modified with latent thiol groups. Biomaterials 23(24): 4753-4758, Dec 2002.
Fishbein I, Chorny M, Banai S, Levitzki A, Danenberg HD, Gao J, Chen X, Moerman E, Gati I, Goldwasser V, Golomb G.: Formulation and delivery mode affect disposition and activity of tyrphostin-loaded nanoparticles in the rat carotid model. Arterioscler Thromb Vasc Biol 21(9): 1434-1439, Sep 2001.
Fishbein I, Brauner R, Chorny M, Gao J, Chen X, Laks H, Golomb G.: Local delivery of mithramycin restores vascular reactivity and inhibits neointimal formation in injured arteries and vascular grafts. J Control Release 77(3): 167-181, Dec 2001.
Fishbein I, Chorny M, Rabinovich L, Banai S, Gaty I, Golomb G.: Nanoparticulate delivery system of a tyrphostin for the treatment of restenosis. J Control Release 65(1-2): 221-229, Mar 2000.
Fishbein I, Waltenberger J, Rabinovich L, Chorny M, Banai S, Levitzki A, Gazit A, Huber R, Hertz SD, Golomb G.: Local delivery of PDGF-receptor-specific tyrphostin inhibits neointimal formation in rats. Arterioscler Thromb Vasc Biol 20(3): 667-676, Mar 2000.
Cohen H, Levy RJ, Gao J, Fishbein I, Kousaev V, Sosnowski S, Slomkowski S, Golomb G.: Sustained delivery and expression of DNA encapsulated in polymeric nanoparticles. Gene Ther 7(22): 1896-1905, Nov 2000.
Banai S, Wolf Y, Golomb G, Pearle A, Waltenberger J, Fishbein I, Schneider A, Gazit A, Perez L, Huber R, Lazarovichi G, Rabinovich L, Levitzki A, Gertz SD.: PDGF-receptor tyrosine kinase blocker AG1295 selectively attenuates smooth muscle cell growth in vitro and reduces neointimal formation after balloon angioplasty in swine. Circulation 97(19): 1960-1969, May 1998.
Brauner R, Lax H, Drinkwater DC, Chaudhuri G, Shvartz O, Drake T, Bhuta S, Mishaly D, Fishbein I, Golomb G.: Controlled periadventitial administration of verapamil inhibits neointimal smooth muscle cell proliferation and ameliorates vasomotor abnormalities in experimental vein bypass grafts. J Thorac Cardiovasc Surg 114(1): 53-63, Jul 1997.
Mishaly D, Fishbein I, Moscovitz D, Golomb G.: Site-specific delivery of colchicine in rat carotid artery model of restenosis. J Control Release 45(1): 65-73, Mar 1997.
Golomb G, Fishbein I, Banai S, Mishaly D, Moscovitz D, Gertz SD, Gazit A, Poradosu E, Levitzki A.: Controlled delivery of a tyrphostin inhibits intimal hyperplasia in a rat carotid artery model. Atherosclerosis 125(2): 171-182, Sep 1996.
Tengood JE, Fishbein I, Levy RJ, Chorny M: Nanomedicines for restenosis treatment. Handbook of Nanobiomedical Research. World Scientific Publishing, Page: 39-87, Oct 2014 Notes: Chapter 2.
Fishbein I, Chorny M, Alferiev IS, Levy RJ: Site specific controlled release for cardiovascular disease - translational directions. Fundamentals and Applications of Controlled Release Drug Delivery. J. Siepmann, M. J.Rathbone, R. A. Siegal (eds.). Springer, Page: 445-492, Nov 2012.
Fishbein I, Perlstein I, Levy RJ.: Gene therapy stents. In: Polymeric Gene Delivery: Principles and Applications. Mansoor M. Amiji (eds.). CRC Press, Page: 625-658, Oct 2005.
Fishbein I, Chorny M, Gaty I, Rabinovich L, Golomb G.: Delivery systems for the treatment of restenosis. Encyclopedic Handbook of Biomaterials and Bioengineering. Wise DL. (eds.). Marcel Dekker, NY, Oct 1999.
Golomb G, Moscovitz D, Fishbein I, Mishaly D.: Perivascular polymeric controlled-release therapy. Encyclopedic Handbook of Biomaterials and Bioengineering. Wise DL. (eds.). Marcel Dekker, NY, Page: 1043-1055, Nov 1995.
Posters and Presentations
Aghali (Elhashmi) A, Keeney S, Fitzpatrick E, Shukla H, Castillero E, Fishbein I, Ferrari G, Levy RJ.: Serotonin Related Gene Expression Patterns in Aortic Valve Interstitial Cells Are Altered by Proliferation and Serotonin Transporter Inhibition. AHA Scientific Sessions 2019; November 16-18, Philadelphia, PA Nov 2019 Notes: poster presentation.
Inamdar VV, Fitzpatrick E, Alferiev I, Nagasami C, Levy RJ, Fishbein I, Stachelek SJ.: Peptide CD47 functionalization of metal surfaces prevents inflammatory cell attachment, activation and promotes anti-inflammatory phenotype in macrophages. AHA Scientific Sessions 2019; November 16-18, Philadelphia, PA Nov 2019 Notes: poster presentation.
Pressly BB, Hooshdaran B, Alferiev IS, Zoltick P, Wilensky RL, Gorman R, Smith JD, Hazen SL, Levy R, Fishbein I.: The Incorporation Of Oxidation Resistant ApoA1-4WF In Adeno-Associated Viral And Lentiviral Gene Vectors With Robust Cholesterol Efflux In Vitro. AHA Scientific Sessions 2019; November 16-18, Philadelphia, PA Nov 2019 Notes: poster presentation
Guerrero DT, Alferiev IS, Nguyen F, Guan P, Kolla V, Soberman D, Fishbein I, Levy RJ, Brodeur GM, Chorny M: Nanocarriers with reversibly hydrophobized co-drugs for treating aggressive neuroblastoma. 2nd International Conference on Pharmaceutical Nanotechnology & Nanomedicine,New York, NY March 2019 Notes: poster presentation.
Inamdar VV, Fitzpatrick E, Alferiev I, Nagaswami C, Levy RJ, Fishbein I, Stachelek SJ: Enhanced Biocompatibility of Stents via CD47 Surface Functionalization. Society for Biomaterials 2019 Annual Meeting, April 3-6, Seattle, WA Apr 2019 Notes: podium presentation.
Alferiev IS, Guerrero DT, Nguen F, Guan P, Kolla V, Soberman D, Fishbein I, Levy RJ, Brodeur GM, Chorny M: Poloxamer-based polymeric prodrugs as an experimental therapy for aggressive solid tumors. Conference on Polymers in Life Sciences; Philadelphia, PA May 2019 Notes: poster presentation.
Guerrero DT, Alferiev IS, Nguen F, Guan P, Kolla V, Soberman D, Fishbein I, Levy RJ, Brodeur GM, Chorny M: Formulation and evaluation of biodegradable polymeric nanoparticles as injectable anticancer co-drug carriers. Conference on Polymers in Life Sciences, Philadelphia, PA May 2019 Notes: poster presentation.
Hooshdaran B, Pressly BB, Alferiev IS, Wilensky RL, Gorman RC, Smith JD, Hazen S, Levy RJ, Fishbein I: Stent-based gene therapy of restenosis with an oxidation-resistant apoA1 mutant. AHA Vascular Discovery Annual Meeting, 2019, May 14-16, Boston, MA May 2019 Notes: podium presentation.
Inamdar VV, Fitzpatrick E, Alferiev IS, Nagaswami C, Levy RJ, Fishbein I, Stachelek SJ: CD47 surface functionalization increases the biocompatibility of metal stents. AHA: Vascular Discovery Annual Meeting, 2019 May 14-16, Boston, MA May 2019 Notes: poster presentation.
Pressly BB, Guerrero DT, Alferiev IS, Hooshdaran B, Kuoch M, Chorny M, Fishbein I: Induction of a Prohealing Macrophage Phenotype with Stent-eluted Gene Vectors for the Prevention of Restenosis. AHA: Vascular Discovery Annual Meeting, 2019 May 14-16, Boston, MA May 2019 Notes: poster presentation.
Guerrero DT, Alferiev IS, Nguen F, Guan P, Kolla V, Soberman D, Fishbein I, Levy RJ, Brodeur GM, Chorny M: Codrug-impregnated nanocarriers as an experimental therapy for aggressive neuroblastoma. International Conference on Pharma Research and Development; Philadelphia, PA Jun 2018 Notes: poster presentation.
Guerrero DT, Alferiev IS, Nguen F, Guan P, Kolla V, Soberman D, Fishbein I, Levy RJ, Brodeur GM, Chorny M: Mutual prodrugs for treating aggressive neuroblastoma with biodegradable nanocarriers. 256th American Chemical Society National Meeting: Nanoscience, Nanotechnology & Beyond; Boston, MA August 2018 Notes: poster presentation.
Awards and Honors
2005, Innovation Award at Stokes Fellows Research Poster Day, Children's Hospital of Philadelphia
1997, Goldhaber Family Award for Academic Achievements, Hebrew University of Jerusalem
1996, The Nagai Foundation Graduate Student Award, Controlled Release Societyl
Editorial and Academic Positions
2020-present, Reviewer: Bioengineering
2020-present, Reviewer: Physiological research
2019-present, Reviewer: Scientific Reports
2019-present, Reviewer: Journal of Cardiovascular Medicine and Cardiology
2019-present, Reviewer: Experimental and Therapeutic Medicine
2019-present, Reviewer: Genes
2018-present, Reviewer: Journal of Exploratory Research in Pharmacology
2017-present, Reviewer: Journal of Pragmatic and Observational Research
2017-present, Reviewer: Molecular Medicine Reports
2016-present, Reviewer: Immunological Investigations
2016-present, Reviewer: BMC Cardiovascular Disorders
2016-present, Reviewer: ACS Nano
2016-present, Reviewer: Cardiovascular Pathology
2015-present, Reviewer: International Journal of Molecular Medicine
2015-present, Reviewer: Journal of Cardiovascular Diseases and Diagnosis
2015-present, Reviewer: Biomaterials
2014-present, Reviewer: Nanomedicine: Nanotechnology, Biology and Medicine
2014-present, Reviewer: Gene Therapy
2014-present, Reviewer: Journal of Clinical and Experimental Cardiology
2014-present, Reviewer: Tissue engineering
2014-present, Reviewer: Journal of Investigative Medicine
2014-present, Reviewer: Pediatric Research
2014-present, Reviewer: Molecular Therapy
2013-present, Reviewer: Nanotechnology
2013-present, Reviewer: Expert Review of Medical Devices
2013-present, Reviewer: Theranostics
2013-present, Editorial Board Member: "Angiology: open access"
2012-present, Reviewer: Molecular Pharmaceutics
2011-present, Reviewer: Expert Opinion on Drug Delivery
2010-present, Reviewer: Journal of Biomedical Materials Research
2009-present, Reviewer: Human Gene Therapy
2009-present, Reviewer: Journal of Drug Delivery Science and Technology
2008-present, Reviewer: Journal of Gene Medicine
2007-present, Reviewer: Nanomedicine
Academic and Institutional Committees
2013-present, Clinical and Scientific Advisory Committee (CSAC) for the Pennsylvania Pediatric Device Consortium (PPDC)
2010-present, Voting Member, Scholarship Oversight Committee, Division of Cardiology
Leadership and Memberships
Memberships in Professional Organizations
2018-present, National Institute of Health
- 2018-present, Vascular Cell and Molecular Biology Study Section, Ad hoc reviewer
2006-present, American Heart Association
2005-present, American Society of Gene and Cell Therapy
Patient Experience Ratings
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