Gerald B. Wertheim, MD, PhD

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Gerald B. Wertheim, MD, PhD, is Director of Hematopathology and a pathologist with the Hematopathology Laboratory at Children's Hospital of Philadelphia.

Locations: Main Hospital
Appointments and Referrals: 1-800-TRY-CHOP (1-800-879-2467)

Education and Training

Medical School

MD, PhD - University of Pennsylvania School of Medicine, Philadelphia, PA

Residency

Anatomic and Clinical Pathology - Hospital of the University of Pennsylvania, Philadelphia, PA

Fellowship

Hematopathology - Hospital of the University of Pennsylvania, Philadelphia, PA

Board Certification

Anatomic Pathology
Hematopathology

Undergraduate Degree

BS in Chemistry - Yale University, New Haven, CT

Titles and Academic Titles

Director of Hematopathology

Pathologist

Laboratory Director, Division of Genomic Diagnostics

Associate Professor of Clinical Pathology and Laboratory Medicine, Perelman School of Medicine at the University of Pennsylvania

Departments and Services

Publications

Papers

2021

Kadauke S, Myers RM, Li Y, Aplenc R, Baniewicz D, Barrett DM, Barz Leahy A, Callahan C, Dolan JG, Fitzgerald JC, Gladney W, Lacey SF, Liu H, Maude SL, McGuire R, Motley LS, Teachey DT, Wertheim GB, Wray L, DiNofia AM, Grupp SA: Risk-Adapted Preemptive Tocilizumab to Prevent Severe Cytokine Release Syndrome After CTL019 for Pediatric B-Cell Acute Lymphoblastic Leukemia: A Prospective Clinical Trial. Journal of Clinical Oncology 39(8): 920-930, January 2021 Notes: Online ahead of print.

Zhao X, Kotch C, Fox E, Surrey LF, Wertheim GB, Baloch ZW, Lin F, Pillai V, Luo M, Kreiger PA, Pogoriler JE, Linn RL, Russo PA, Santi M, Resnick AC, Storm PB, Hunger SP, Bauer AJ and Li MM: NTRK Fusions Identified in Pediatric Tumors: The Frequency, Fusion Partners, and Clinical Outcome. JCO Precision Oncology 5: 204-214, January 2021.

Regina M Myers, Yimei Li, Allison Barz Leahy, David M Barrett, David T Teachey, Colleen Callahan, Christina C Fasano, Susan R Rheingold, Amanda DiNofia, Lisa Wray, Richard Aplenc, Diane Baniewicz, Hongyan Liu, Pamela A Shaw, Edward Pequignot, Kelly D Getz, Jennifer L Brogdon, Andrew D Fesnak, Donald L Siegel, Megan M Davis, Chelsie Bartoszek, Simon F Lacey, Elizabeth O Hexner, Anne Chew, Gerald B Wertheim, Bruce L Levine, Carl H June, Stephan A Grupp, Shannon L Maude: Humanized CD19-Targeted Chimeric Antigen Receptor (CAR) T Cells in CAR-Naive and CAR-Exposed Children and Young Adults With Relapsed or Refractory Acute Lymphoblastic Leukemia. Journal of Clinical Oncology June 2021 Notes: DOI: 10.1200/JCO.20.03458.

2020

Balci TB, Strong A, Kalish JM, Zackai E, Maris JM, Reilly A, Surrey LF, Wertheim GB, Marcadier JL, Graham GE, Carter MT: Tatton-Brown-Rahman syndrome: Six individuals with novel features. American Journal of Medical Genetics January 2020.

Oberley MJ, Raikar SS, Wertheim GB, Malvar J, Sposto R,Rabin KR, Punia JN, Seif AE, Cahen VC, Schore RJ, Luca DC, Guinipero T, Woods WG, O'Gorman MRG, Orgel E: Significance of minimal residual disease in pediatric mixed phenotype acute leukemia: a multicenter cohort study. Leukemia February 2020 Notes: Epub ahead of print.

Arenas DJ, Floess K, Kobrin D, Pai RL, Srkalovic MB, Tamakloe MA, Rasheed R, Ziglar J, Khor J, Parente SAT, Pierson SK, Martinez D, Wertheim GB, Kambayashi T, Baur J, Teachey DT, Fajgenbaum DC: Increased mTOR activation in idiopathic multicentric Castleman disease. Blood 135(19): 1673‐1684, March 2020 Notes: Epub ahead of print.

Bellissimo DC, Chen CH, Zhu Q, Bagga S, Lee CT, He B, Wertheim GB, Jordan M, Tan K, Worthen GS, Gilliland DG, Speck NA: Runx1 negatively regulates inflammatory cytokine production by neutrophils in response to Toll-like receptor signaling. Blood Advances 4(6): 1145-1158, March 2020.

Cai Z, Aguilera F, Ramdas B, Daulatabad SV, Srivastava R, Kotzin JJ, Carroll M, Wertheim G, Williams A, Janga SC, Zhang C, Henao-Mejia J, Kapur R.: Targeting Bim via a lncRNA Morrbid Regulates the Survival of Preleukemic and Leukemic Cells. Cell Reports 31(12): 107816, June 2020.

Hurtz C, Wertheim GB, Loftus JP, Blumenthal D, Lehman A, Li Y, Bagashev A, Manning B, Cummins KD, Burkhardt JK, Perl AE, Carroll M, Tasian SK: Oncogene-independent BCR-like signaling adaptation confers drug resistance in Ph-like ALL. The Journal of Clinical Investigation 130(7): 3637-3653, July 2020.

Rentas S, Pillai V, Wertheim GB, Akgumus GT, Nichols KE, Deardorff MA, Conlin LK, Li MM, Olson TS, Luo M   : Evolution of Histomorphologic, Cytogenetic, and Genetic Abnormalities in an Untreated Patient With MIRAGE Syndrome. Cancer Genetics 245: 42-48, July 2020.

Jung HS, Lin F, Wolpaw A, Reilly AF, Margolskee E, Luo M, Wertheim GB, Li MM: A Novel KMT2A-ARHGEF12 Fusion Gene Identified in a High-Grade B-cell Lymphoma. Cancer Genetics Page: 41-43, August 2020 Notes: Online ahead of print.

Kelemen K, Saft L, Craig FE, Orazi A, Nakashima M, Wertheim GB, George TI, Horny HP, King RL, Quintanilla-Martinez L, Wang SA, Rimsza LM, Reichard KK: Eosinophilia/Hypereosinophilia in the Setting of Reactive and Idiopathic Causes, Well-Defined Myeloid or Lymphoid Leukemias, or Germline Disorders. American journal of clinical pathology 155(2): 179-210, December 2020 Notes: Online ahead of print.

2019

Ding YY, Stern JW, Jubelirer TF, Wertheim GB, Li F, Chang F, Gu Z, Mullighan CG, Li Y, Harvey RC, Chen IM, Willman CL, Hunger SP, Li MM, Tasian SK: Clinical efficacy of ruxolitinib and chemotherapy in a child with Philadelphia chromosome-like acute lymphoblastic leukemia with GOLGA5-JAK2 fusion and induction failure. Haematologica 2018 Notes: Epub ahead of print.

Mack EA, Stein SJ, Rome KS, Xu L2, Wertheim GB, Melo RCN, Pear WS: Trib1 regulates eosinophil lineage commitment and identity by restraining the neutrophil program. Blood 133(22): 2413-2426, May 30 2019.

Fajgenbaum DC, Langan RA, Japp AS, Partridge HL, Pierson SK, Singh A, Arenas DJ, Ruth JR, Nabel CS, Stone K, Okumura M, Schwarer A, Jose FF, Hamerschlak N, Wertheim GB, Jordan MB, Cohen AD, Krymskaya V, Rubenstein A, Betts MR, Kambayashi T, van Rhee F, Uldrick TS: Identifying and targeting pathogenic PI3K/AKT/mTOR signaling in IL-6-blockade-refractory idiopathic multicentric Castleman disease. The Journal of Clinical Investigation 130: 4451-4463, Aug 13 2019.

Pillai V, Muralidharan K, Meng W, Bagashev A, Oldridge DA, Rosenthal J, Van Arnam J, Melenhorst JJ, Mohan D, DiNofia AM, Luo M, Cherian S, Fromm JR, Wertheim G, Thomas-Tikhonenko A, Paessler M, June CH, Luning Prak ET, Bhoj VG, Grupp SA, Maude SL, Rheingold SR: CAR T-cell therapy is effective for CD19-dim B-lymphoblastic leukemia but is impacted by prior blinatumomab therapy. Blood Advances 3(22): 3539-3549, November 2019.

2018

Bu Y, Yoshida A, Chitnis N, Altman A, Tameire F, Oran A, Gennaro v, Armeson K, McMahon S, Wertheim G, Dang C, Ruggero D, Koumenis C, Fuchs S, Diehl JA: PERK-miR-211 integrates oncogenic signaling with circadian oscillation and protein synthesis. Nature Cell Biology 20(1): 104-115, Jan 2018.

Sarthy J, Zha J, Babushok D, Shenoy A, Fan JM, Wertheim G, Himebauch A, Munchel A, Taraseviciute A, Yang S, Shima H, Narumi S, Meshinchi S, Olson TS: Poor outcome with hematopoietic stem cell transplantation for bone marrow failure and MDS with severe MIRAGE syndrome phenotype. Blood Adv 2(2): 120-125, Jan 2018.

Krevvata M, Shan X, Zhou C, Dos Santos C, Habineza Ndikuyeze G, Secreto A, Glover J, Trotman W, Brake-Silla G, Nunez-Cruz S, Wertheim G, Ra HJ, Griffiths E, Papachristou C, Danet-Desnoyers G, Carroll M: Cytokines increase engraftment of human acute myeloid leukemia cells in immunocompromised mice but not engraftment of human myelodysplastic syndrome cells. Haematologica 103(6): 959-971, June 2018.

Rosenthal J, Naqvi AS, Luo M, Wertheim G, Paessler M, Thomas-Tikhonenko A, Rheingold SR, Pillai V: Heterogeneity of surface CD19 and CD22 expression in B lymphoblastic leukemia. Am J Hematol July 2018 Notes: Epub ahead of print.

2017

Tasian SK, Hurtz C, Wertheim GB, Bailey NG, Lim MS, Harvey RC, Chen IM, Willman CL, Astles R, Zebrowski A, Reshmi SC, Li MM, Frey NV, Luger SM, Carroll M, Perl AE.: High incidence of philadelphia chromosome-like acute lymphoblastic leukemia (Ph-like ALL) in older adults with B-ALL. Leukemia January 3 2017.

Yu H, Sotillo E, Harrington C, Wertheim G, Paessler M, Maude SL, Rheingold SR, Grupp SA, Thomas-Tikhonenko A, Pillai V.: Repeated loss of target surface antigen after immunotherapy in primary mediastinal large B cell lymphoma. American Journal of Hematology 92(1): E11-E13, January 2017.

DiNardo CD, Luskin MR, Carroll M, Smith C, Harrison J, Pierce S, Kornblau S, Konopleva M, Kadia T, Kantarjian H, Wertheim G, Master SR: Validation of a Clinical Assay of Multi-Locus DNA Methylation for Prognosis of Newly Diagnosed AML. American Journal of Hematology 92(2): E14-E15, Feb  2017 Notes: Co-Senior Author.

King RL, Paessler ME, Howard MT, Wertheim GB: Incidental EBV-positivity in paediatric post-transplant specimens demonstrates the need for stringent criteria for diagnosing post-transplant lymphoproliferative disorders. Journal of Clinical Pathology 70(3): 270-273, Mar 2017.

Xu Y, Wertheim G, Morrissette JJD, Bagg A: BRAF kinase domain mutations in de novo acute myeloid leukemia with monocytic differentiation. Leukemia and Lymphoma 58(3): 743-745, Mar 2017.

Xu Y, Morrissette J, Wertheim GB, Bagg A: BRAF kinase domain mutations in de novo acute myeloid leukemia with monocytic differentiation. Leukemia and Lymphoma  31(4): 9813-3, Apr 2017.

Kim E, Cheng Y, Bolton-Gillespie E, Cai X, Ma C, Tarangelo A, Le L, Jambhekar M, Raman P, Hayer KE, Wertheim G, Speck NA, Tong W, Viatour P: Rb family proteins enforce the homeostasis of quiescent hematopoietic stem cells by repressing Socs3 expression. Journal of Experimental Medicine 214(7): 1901-1912, May 2017.

Green AM, Budagyan K, Hayer KE, Reed MA, Savani MR, Wertheim GB, Weitzman MD: Cytosine deaminase APOBEC3A sensitizes leukemia cells to inhibition of the DNA replication checkpoint. Cancer Research 77(17): 4579-88, Sep 2017.

Grenstein LAV, Coughlin CC, Flynn AT, Pillai V, Boos MD, Wertheim GW, Treat JR, Teachey DT: Severe Mucha-Habermann-like Ulceronecrotic Skin Disease in T-cell ALL Responsive to Basiliximab and Stem Cell Transplant  Pediatric Dermatology 34(5): e265-e270, September 2017.

Pajcini KV, Xu L, Shao L, Petrovic J, Palasiewicz K, Ohtani Y, Bailis W, Lee C, Wertheim GB, Rajeswaran M, Musuthamy N, Li Y, Meijerink JPP, Blacklow SC, Faryabi RB, Cherry S and Pear WS: MafB is a novel enhancer of oncogenic Notch signaling in T-cell acute lymphoblastic leukemia. Science Signaling  10(505), November 2017.

Lalonde E, Wertheim G, Li MM: Clinical Impact of Genomic Information in Pediatric Leukemia. Front Pediatr 5: 263, December 2017.

2016

Bhoj VG, Arhontoulis D, Wertheim G, Capobianchi J, Callahan CA, Ellebrecht CT, Obstfeld AE, Lacey SF, Melenhorst JJ, Nazimuddin F, Hwang WT, Maude SL, Wasik MA, Bagg A, Schuster S, Feldman MD, Porter DL, Grupp SA, June CH, Milone MC: Persistence of long-lived plasma cells and humoral immunity in individuals responding to CD19-directed CAR T-cell therapy. Blood 128(3): 360-370, January 2016.

Luskin M, Gimotty PA, Smith C, Loren A, Figueroa M, Harrison J, Sun Z, Tallman M, Paietta E, Litzow M, Melnick A, Levine RL, Fernandez H, Luger S, Carroll M, Master S, Wertheim G: A clinical measure of DNA methylation predicts outcome in de novo acute myeloid leukemia. JCI Insight 1(9), Jun 16 2016.

Teachey DT, Lacey SF, Shaw PA, Melenhorst JJ, Maude SL, Frey N, Pequignot E, Gonzalez VE, Chen F, Finklestein, J, Barrett DM, Weiss, SL, Fitzgerald JC, Berg, RA MD, Aplenc, R, Callahan C, Rheingold, SR, Zheng Z, Rose-John S, Nazimuddin F, Wertheim GB, Levine BL, June CH, Porter DL, Grupp SA : Identification of Predictive Biomarkers for Cytokine Release Syndrome after Chimeric Antigen Receptor T cell Therapy for Acute Lymphoblastic Leukemia  Cancer Discovery 6(6): 667-79, Jun 2016.

2015

Wertheim GB, Smith C, Luskin M, Rager A, Figueroa ME, Carroll M, Master SR: Validation of DNA Methylation to Predict Outcome in Acute Myeloid Leukemia by Use of xMELP. Clinical Chemistry 61(1): 249-258, Jan 2015.

DeMicco A, Naradikian MS, Sindhava VJ, Yoon JH, Gorospe M, Wertheim GB, Cancro MP, Bassing CH: B Cell-Intrinsic Expression of the HuR RNA-Binding Protein Is Required for the T Cell-Dependent Immune Response In Vivo. Journal of Immunology 195(7): 3449-62, October 2015.

2014

Choi, SM, Papenhausen P, Wertheim G, and King RL: Near-haploid B lymphoblastic leukemia with an apparent hyperdiploid karyotype: the critical role of SNP analysis in establishing proper diagnosis. Journal Of Hematopathology 2014.

Gerhardt DM, Pajcini KV, D'altri T, Tu L, Jain R, Xu L, Chen M, Rentschler S, Shestova O, Wertheim GB, Tobias J, Kluk M, Wood AW, Aster JC, Gimotty PA, Epstein JA, Speck N, Bigas A, Pear WS : The Notch1 transcriptional activation domain is required for development and reveals a novel role for Notch1 signaling in fetal hematopoietic stem cells. Genes and Development 2014.

Luskin M*, Wertheim G*, Morrissette J, Daber R, Biegel J, Wilmoth D, Kersun L, King R, Paessler M, Simon C, Aplenc R and Loren A*. Authors contributed equally: CLL/SLL diagnosed in an adolescent. Pediatric Blood & Cancer 2014.

O'Brien S, Rajan MT, Wertheim G, Wells A: Ikaros imposes a barrier to CD8+ T cell differentiation by restricting autocrine IL-2 production. Journal of Immunology 2014.

Raess, PW, van de Geijn GJM, Njo TL, Klop B, Sukhachev D, Wertheim G, McAleer T, Master SR, Bagg A.: Automated screening for myelodysplastic syndromes through analysis of complete blood count and cell population data parameters. American Journal of Hematology 2014.

Wertheim, GBW, Smith, C, Figueroa, ME, Kalos, M, Bagg, A, Carroll M, and Master, SR: Microsphere-based Multiplex Analysis of DNA Methylation in Acute Myeloid Leukemia. Journal of Mol. Diagnostics 2014.

2012

Wertheim Gerald BW, Daber R, Bagg A: Molecular Diagnostics of Acute Myeloid Leukemia: It's a (Next) Generational Thing. The Journal of molecular diagnostics : JMD Nov 2012.

Wertheim GBW, Hexner E, Bagg A: Molecular-Based Classification of Acute Myeloid Leukemia and Its Role in Directing Rational Therapy: Personalized Medicine for Profoundly Promiscuous Proliferations. Molecular diagnosis & therapy Nov 2012.

2011

Chi AWS, Chavez A, Xu L, Weber BN, Shestova O, Shaffer A, Wertheim GB, Pear WS, Izon D, Bhandoola A: Identification of Flt3+CD150- myeloid progenitors in adult mouse bone marrow that harbor T lymphoid developmental potential. Blood 118(10): 2723-32, Jul 2011.

2009

Wertheim GB, Yang TW, Pan TC, Ramne A, Liu Z, Gardner HP, Dugan KD, Kristel P, Kreike B, Van de Vijver MJ, Cardiff RD, Reynolds C, Chodosh LA: The Snf1-related kinase, Hunk, is essential for mammary tumor metastasis. Proceedings of the National Academy of Sciences of the United States of America 106(37): 15855-60, Sep 2009.

2008

Wertheim GB, Bagg A: Nucleophosmin (NPM1) mutations in acute myeloid leukemia: an ongoing (cytoplasmic) tale of dueling mutations and duality of molecular genetic testing methodologies. The Journal of Molecular Diagnostics  10(3): 198-202, May 2008.

2002

Gunther EJ, Belka GK, Wertheim GB, Wang J, Hartman JL, Boxer RB, Chodosh LA: A novel doxycycline-inducible system for the transgenic analysis of mammary gland biology. The FASEB journal : official publication of the Federation of American Societies for Experimental Biology 16(3): 283-92, Mar 2002.

2000

Gardner H P, Wertheim G B, Ha S I, Copeland N G, Gilbert D J, Jenkins N A, Marquis S T, Chodosh L A: Cloning and characterization of Hunk, a novel mammalian SNF1-related protein kinase. Genomics 63(1): 46-59, Jan 2000.

Gardner H P, Belka G K, Wertheim G B, Hartman J L, Ha S I, Gimotty P A, Marquis S T, Chodosh L A: Developmental role of the SNF1-related kinase Hunk in pregnancy-induced changes in the mammary gland. Development (Cambridge, England) 127(20): 4493-509, Oct 2000.

Abstracts

2021

Lytle A, Pillai V, Wertheim GW, Paessler M, Croy C and Rheingold S: Blinatumomab Induces Bone Marrow Lymphoid Infiltrates That Correlate with Response in B-ALL. United States and Canadian Academy of Pathology 2021 Baltimore, Maryland March 2021.

Wei J, Faude S, Xu X, Wertheim G, Paessler M, Bhoj V, Pillai V: Morphological and Immunophenotypic Characterization of CAR T Cells. United States and Canadian Academy of Pathology 2021 Baltimore, Maryland March 2021.

2020

Faude S, Pillai V, Paessler ME, Wertheim GBW: Absolute Lymphocyte Count Kinetics Correlates with Response and Toxicity in CAR T Therapy. United States and Canadian Academy of Pathology 2020 Los Angeles, California March 2020.

Nelson ND, Meng W, Rosenfeld A, Bullman S, Pedamullu CS, Nomburg JL, Wertheim GBW, Paessler, ME, Pinkus GS, Hornick JL, Meyerson ML, Prak EL, Pillai V: Characterization of Plasmacytoid Dendritic Cells, Microbial Sequences, and Identification of a Candidate Public T-cell Clone in Kikuchi-Fujimoto Lymphadenitis. United States and Canadian Academy of Pathology 2020 Los Angeles, California March 2020.

Yang G, Faude S, Paessler ME, Wertheim GBW, Pillai V: CD19-Negative B-ALL Relapses after CAR T Cell Therapy are Associated with a Hypermutator Phenotype  United States and Canadian Academy of Pathology 2020 Los Angeles, California March 2020.

2019

Modi M, Cotner C, Wertheim G, Paessler M, Tasian SK, Luo M: Clusters of CD123-Positive Plasmacytoid Dendritic Cells in JMML. United States and Canadian Academy of Pathology 2019 MeetingNational Harbor, Maryland, 2019 March 2019.

Books

Chapters

2021

Margolskee E, Wertheim GBW, Harvey RC: Pathology and Molecular Diagnosis of Leukemias and Lymphomas. Pizzo and Poplack's Pediatric Oncology 8th Edition Page: 117-128, 2021.

2011

Wertheim GB, Bagg A: Minimal residual disease testing to predict relapse following transplant for AML and high-grade myelodysplastic syndromes. Expert review of molecular diagnostics 11(4): 361-6, May 2011.

Posters and Presentations

Invited Lectures

2021

Wertheim G B. "Pediatric Hematopathology Cases", Thomas Jefferson University Hospital, Philadelphia, PA (Due to COVID lecture was virtual). Jan 2021.

Awards and Honors

2012, International Society of Laboratory Hematology; One Top Five Abstracts at Society's International Meeting; Nice, France.
2005, Endocrinology Research Award, University of Pennsylvania School of Medicine
1996, The Stella Botelho Award in Physiology, University of Pennsylvania School of Medicine
1995-2005, 21st Century/Gamble Scholarship (Full Four-Year Medical School Tuition), University of Pennsylvania School of Medicine
1994, The Werner Bergmann Award in Chemistry, Yale University
1993, Phi Beta Kappa, Yale University

Editorial and Academic Positions

Editorial positions

2021-present, Ad Hoc Reviewer, Leukemia Research
2020-present, Ad Hoc Reviewer, Blood
2017-present, Ad Hoc Reviewer, Trends in Cancer
2017-present, Ad Hoc Reviewer, American Journal of Clinical Pathology
2016-present, Ad Hoc Reviewer, Clinical Chemistry and Laboratory Medicine
2016-present, Ad Hoc Reviewer, Histopathology
2016-present, Ad Hoc Reviewer, Molecular and Cellular Biochemistry
2016-present, Member, Editorial Board, Journal of Molecular Diagnostics
2015-present, Ad Hoc Reviewer, FASEB Journal
2011-present, Journal of Molecular Diagnostics, Ad Hoc Reviewer
2011-present, Cancer Genetics, Ad Hoc Reviewer

Academic and institutional committees

2020-present, Program Member, Undergraduate Medical Education (UME) Curriculum Development Team - Hematology Block, Vingroup-Penn Alliance, Perelman School of Medicine, University of Pennsylvania
2016-present, Biobanking Committee
2015-present, Hematopathology Clinical Competency Committee, Chair

Leadership and Memberships

Memberships in Professional Organizations

International

2015-present, Society of Hematopathology
2015-present, The United States and Canadian Academy of Pathology

National

2015-present, American Society of Hematology