Stephen R. Master, MD, PhD

Stephen R. Master, MD, PhD
Stephen R. Master, MD, PhD, is the Division Chief and Director of Metabolic and Advanced Diagnostics at Children's Hospital of Philadelphia.

Locations: Main Campus
Appointments and Referrals: 1-800-TRY-CHOP (1-800-879-2467)

Education and Training

Medical School

MD - University of Pennsylvania School of Medicine, Philadelphia, PA

Residency

Clinical Pathology - Hospital of the University of Pennsylvania, Philadelphia, PA

Board Certification

Clinical Informatics
Clinical Pathology

Graduate Degree

PhD in Cell and Molecular Biology - University of Pennsylvania School of Medicine, Philadelphia, PA
MS in Translational Research - University of Pennsylvania, Philadelphia, PA

Titles and Academic Titles

Division Chief and Director, Metabolic and Advanced Diagnostics

Associate Professor of Pathology and Laboratory Medicine, Perelman School of Medicine at the University of Pennsylvania

Departments and Services

Publications

Papers

2017

Wertheim GB, Luskin MR, Carroll M, Master SR: Microsphere-based assessment of DNA methylation for AML prognosis. Methods Mol Biol(1633), 125-136, 2017.

Niazi MKK, Chung JH, Heaton-Johnson KJ, Martinez D, Castellanos R, Irwin MS, Master SR, Pawel BR, Gurcan MN, Weiser DA: Advancing clinicopathologic diagnosis of high-risk neuroblastoma using computerized image analysis and proteomic profiling. Pediatr Dev Pathol Jul, 2017 Notes: Epub ahead of print.

Master CL, Master SR, Wiebe DJ, Storey EP, Lockyer JE, Podolak OE, Grady MF: Vision and Vestibular System Dysfunction Predicts Prolonged Concussion Recovery in Children. Clin J Sport Med Oct 2017.

2016

Luskin ML, Gimotty PA, Smith C, Loren AW, Figueroa ME, Harrison J, Sun Z Tallman MS, Paietta EM, Litzow MR, Melnick A, Levine R, Fernandez HF, Luger SM, Carroll M, Master SR *, Wertheim GBW *.  : A Clinical Measure of DNA Methylation Predicts Outcome in De Novo AML. JCI Insight 1(9), 2016 Notes: Both authors contributed equally.

Garrigan C, Han J, Tolomeo P, Johnson KJ, Master SR, Lautenbach E, Nachamkin I: Evaluation of a research use only luminex based assay for measurement of procalcitonin in serum samples. Am J Trasl Res 8(10): 4362-4369, 2016.

DiNardo CD #, Luskin MR #, Carroll M, Smith C, Harrison J, Pierce S, Kornblau S, Konopleva M, Kadia T, Katarjian H, Wertheim GM *, Master SR : Validation of a Clinical Assay of Multi-Locus DNA Methylation for Prognosis of Newly Diagnosed AML   Am J Hematol 8(10): 4362-67, 2016 Notes: #Both authors contributed equally.

Storey EP, Master SR, Lockyer JE, Podolak OE, Grady MF, Master CL: Near Point of Convergence after Concussion in Children. Optom Vis Sci 94(1): 96-100, July  2016.

Master CL, Scheiman M, Gallaway M, Goodman A, Robinson RL, Master SR, Grady MF: Vision Diagnoses are Common after Concussion in Adolescents. Clin Pediatr 55(3): 260-7, Mar 2016.

2015

Shah R, Matthews GJ, Shah RY, McLaughlin C, Chen J, Wolman M, Master SR, Chai B, Xie D, Rader DJ, Raj DS, Mehta NN, Budoff M, Fischer MJ, Go AS, Townsend RR, He J, Kusek JW, Feldman HI, Foulkes AS, Reilly MP: Study Investigators. Serum Fractalkine (CX3CL1) and Cardiovascular Outcomes and Diabetes: Findings from the Chronic Renal Insufficiency Cohort (CRIC) Study. Am J Kidney Dis 66(2): 266-73, Aug 2015.

Arsenault PR, Heaton-Johnson KJ, Li LS, Song D, Ferreira VS, Patel N, Master SR (*), Lee FS (*): Identification of Prolyl Hydroxylation Modifications in Mammalian Cell Proteins. Proteomics 15(7): 1259-67, Apr 2015.

Wei S, Bing Z Yao Y, Master SRM: High Expression of miR-182 in Cytology Specimens of High-Grade Urothelial Cell Carcinoma: A Potential Diagnostic Marker. Acta Cytol 59(1): 109-12, Feb 2015.

Liu KD, Yang W, Go AS, Anderson AH, Feldman HI, Fischer MJ, He J, Kallem RR, Kusek JW, Master SR, Miller ER, Rosas SE, Steigerwalt S, Tao K, Weir MR, Hsu CY: Urine Neutrophil Gelatinase-Associated Lipocalin and Risk of Cardiovascular Disease and Death in CKD: Results From the Chronic Renal Insufficiency Cohort (CRIC) Study. Am J Kidney Dis 65(2): 267-74, Feb 2015.

Wertheim GBW, Smith C, Luskin M, Rager A, Figueroa ME, Carroll M, Master SR: Validation of DNA Methylation to Predict Outcome in Acute Myeloid Leukemia using xMELP. Clin Chem 61(1): 249-58, January 2015.

2014

Mehta NN, Matthews G,Krishnamoorthy P,Shah R,McLaughlin C,Patel P,Budoff M,Chen J,Wolman M,Go A,He J,Kanetsky PA,Master S,Rader DJ,Raj D,Gadegbeku CA,Shah R,Schreiber M,Fischer MJ,Townsend RR,Kusek J,Feldman HI,Foulkes A, Reilly MP,and the Chronic Renal Insufficiency Cohort (CRIC) Study Investigators: Higher plasma CXCL12 levels predict incident myocardial infarction and death in chronic kidney disease: Findings from the Chronic Renal Insufficiency Cohort Study. Eur Heart J 35(31): 2115-22, Aug 2014.

Babushok DV, Cardamone D, Rulander N, Master SR, Hexner EO: A Common, Under-Recognized Cause of Elevated Serum Erythropoietin. Am J Med Page: S0002-9343(14)00685-8, Aug 2014.

Wing MR, Yang W, Teal V, Navaneethan S, Tao K, Ojo A, Guzman NN, Reilly M, Wolman M, Rosas SE, Cuevas M, Fischer M, Lustigova E, Master SR, Xie D, Appleby D, Joffe M, Kusek J, Feldman HI, Raj DS; for the Chronic Renal Insufficiency Cohort (CRIC) Study.: Race modifies the association between adiposity and inflammation in patients with chronic kidney disease: Findings from the CRIC study. Obesity (Silver Spring) 22(5): 1359-66, May 2014.

Song D, Li LS, Arsenault PR, Heaton-Johnson KJ, Master SR, Lee FS: Defective Tibetan PHD2 Binding to p23 Links High Altitude Adaptation to Altered Oxgen Sensing. J Biol Chem 289(21): 14656-65, May 2014.

Raess P, ven de Geijn G-J, Njo T, Klop B, Sukhachev D, Wertheim G, McAleer T, Master SR (*), Bagg A (*): Automated screening for myelodysplastic syndromes through analysis of complete blood count and cell population data parameters. Am J Hematology 89(4): 369-74, Apr 2014 Notes: (*) co-corresponding authors, contributed equally.

Wertheim GBW, Smith C, Figueroa ME, Kalos M, Bagg A, Carroll M, Master SR: Microsphere-based Multiplex Analysis of DNA Methylation in Acute Myeloid Leukemia. J Mol Diagn 16(2): 207-15, Mar 2014.

2013

Rulander NJ,Cardamone D,Senior M,Snyder PJ,Master SR: Interference from Anti-Streptavidin Antibody. Arch Pathol Lab Med 137(8): 1141-6, Aug 2013.

Ferguson JF, Matthews GJ, Townsend RR, Raj DS, Kanetsky PA, Budoff M, Fischer MJ, Rosas SE, Kanthety R, Rahman M, Master SR, Qasim A, Li M, Mehta NN, Shen H, Mitchell BD, O'Connell JR, Shuldiner AR, Ho WK, Young R, Rasheed A, Danesh J, He J, Kusek JW, Ojo AO, Flack J, Go AS, Gadegbeku CA, Wright JT Jr, Saleheen D, Feldman HI, Rader DJ, Foulkes AS, Reilly MP; CRIC Study Principal Investigators.: Candidate gene association study of coronary artery calcification in chronic kidney disease: findings from the CRIC study (Chronic Renal Insufficiency Cohort). J Am Coll Cardiol 62(9): 789-98, Aug 2013.

Scialla JJ*, Lau WL*, Reilly MP, Isakova T, Yang HY, Crouthamel MH, Chavkin NW, Rahman M, Wahl P, Amaral AP, Hamano T, Master SR, Nessel L, Chai B, Xie D, Kallem RR, Chen J, Lash JP, Kusek JW, Budoff MJ, Giachelli CM, Wolf M: Fibroblast Growth Factor 23 is not associated with and does not induce arterial calcification. Kidney Int 83(6): 1159-68, Jun 2013.

Platt JM, Ryvkin P, Wannat J, Donahue G, Ricketts MD, Barrett S, Song S, Chavez A, Abdallah K, Master SR, Wang LS, Johnson FB: Rap1 relocalization contributes to the chromatin-mediated gene expression profile and pace of cell senescence. Genes Dev 27(12): 1406-20, Jun 2013.

Song D, Li LS, Heaton-Johnson KJ, Arsenault PR, Master SR, Lee FS: Prolyl Hydroxylase Domain Protein 2 (PHD2) Binds a Pro-Xaa-Leu-Glu Motif, Linking it to the Heat Shock Protein 90 Pathway. J Biol Chem 288(14): 7662-74, Apr 2013.

Ferguson JF, Patel PN, Shah RY, Mulvey CK, Gadi R,Nijjar PS,Usman HM,Mehta NM,Shah R,Master SR,Propert KJ,Reilly MP: Race and gender variation in response to evoked inflammation. J Trans Med 11: 63, Mar 2013.

Bose S, Krishnamoorthy P,Varanasi A, Nair J,Schutta M,Braunstein S, Iqbal N, Schwartz S, St. Clair C, Master SR,Rader DJ,Reilly MP,Mehta NN: Measurement of waist circumference predicts coronary atherosclerosis beyond plasma adipokines. Obesity 21((1)): E118-23, Jan 2013.

2012

Bing Z, Master SR, Tobias JW, Baldwin DA, Xu XW, Tomaszewski JE: MicroRNA Expression Profiles of Seminoma from Paraffin-Embedded Formalin-Fixed Tissue. Virchows Archive 461(6): 663-8, Dec 2012.

Bing Z, Li JH, Master SR, Lee CCR, Puthiyveettil R, Tomaszewski JE: Fluorescence In Situ Hybridization of Chromosome Abnormalities of Upper Urinary Tract Urothelial Carcinoma in Paraffin Embedded Tissue. Am J Clin Pathol 138(3): 382-9, Sep 2012.

Mulvey CK, Ferguson JF, Tabita-Martinez J, Kong S, Shah RY, Patel PN, Master SR, Usman MH, Propert KJ, Shah R, Mehta NN, Reilly MP: Peroxisome Proliferator-Activated Receptor-alpha Agonism with Fenofibrate Does Not Suppress Inflammatory Responses to Evoked Endotoxemia. J Am Heart Assoc 1(4): e002923, Aug 2012.

Mehta NN, Heffron SP, Patel PN, Ferguson J, Shah RD, Hinkle CC, Krishnamoorthy P, Shah R, Tabita-Martinez J, Terembula K, Master SR, Rickels MR, Reilly MP: A human model of inflammatory cardio-metabolic dysfunction; a double blind placebo-controlled crossover trial. Journal of Translational Medicine 10: 124, Jun 2012.

Narayan SB, Master SR, Sireci AN, Bierl C, Stanley PE, Li C, Stanley CA, Bennett MJ: Short-Chain 3-Hydroxyacyl-Coenzyme A Dehydrogenase Associates with a Protein Super-Complex Integrating Multiple Metabolic Pathways. PLoS ONE 7(4): e35048, Apr 2012.

Ferguson JF, Hinkle CC, Mehta NN, Bagheri R, DerOhannessian SL, Shah R, Wolfe M, Bradfield JP, Hakonarson H, Wang X, Master SR, Rader DJ, Li M, Reilly MP: Translational studies of lipoprotein-associated phospholipase A2 in inflammation and atherosclerosis. J Am Coll Cardiol 59(8): 764-72, Feb 2012.

2011

Golugula A, Lee G, Master SR, Feldman MD, Tomaszewski JE, Speicher DW, Madabhushi A: Supervised Regularized Canonical Correlation Analysis: Integrating Histologic and Proteomic Measurements for Predicting Biochemical Failures Following Prostate Surgery. BMC Bioinformatics 12(483), Dec 2011.

2010

Romero R, Kusanovic JP, Gotsch F, Erez O, Vaisbuch E, Mazaki-Tovi S, Moser A, Tam S, Leszyk J, Master SR, Juhasz P, Pacora P, Ogge G, Gomez R, Yoon BH, Yeo L, Hassan SS, Rogers W: Isobaric Labeling and Tandem Mass Spectrometry: A Novel Approach for Profiling and Quantifying Proteins Differentially Expressed in Amniotic Fluid in Preterm Labor with and without Intra-amniotic Infection/Inflammation. J Maternal-Fetal and Neonatal Med 23(4): 261-80, Apr 2010.

Everett L, Bierl C, Master SR: Statistical Bias in Multi-Stage Proteomic Search Strategies. J Proteome Res 9(2): 700-707, Feb 2010.

2009

Kricka LJ, Milone M, Master SR, Shaw L, Young DS, Fontanilla R, Smith T, Gardiner J, Cardamone D, Fleming M, Rhoads DG: Effect of a variable magnetic field on clinical laboratory testing. Clin Chem 55: 1249-50, Jun 2009.

Furlow PW, Percy MJ, Sutherland S, Bierl C, McMullin MF, Master SR, Lappin TR, Lee FS: Erythrocytosis-associated HIF-2alpha mutations demonstrate a critical role for residues C-terminal to the hydroxylacceptor proline. J Bio Chem 248(14): 9050-8, Apr 2009.

2008

Orwig KE, Ryu BY, Master SR, Mack M, Avarbock MR, Chodosh LA, Brinster RL: Genes Involved in Post-transcriptional Regulation are Over Represented in Stem/progenitor Spermatogonia of Cryptorchid Mouse Testes. Stem Cells 26(4): 927-938, Apr 2008.

2007

Tetzlaff MT, Liu A, Xu X, Master SR, Baldwin DA, Tobias JW, Livolsi VA, Baloch ZW: Differential Expression of miRNAs in Papillary Thyroid Carcinoma Compared to Multinodular Goiter Using Formalin Fixed Paraffin Embedded Tissues. Endocr Path 18(3): 163-73, 2007.

2006

Wang M *, Master SR *, Chodosh LA: Computational Expression Deconvolution in a Complex Mammalian Organ. BMC Bioinformatics 7: 328, Jul 2006 Notes: * Both authors contributed equally.

2005

Master SR, Stoddard AJ, Bailey LC, Pan TC, Dugan KL, Chodosh LA.: Genomic analysis of early murine mammary gland development using novel probe-level algorithms. Genome Biol 6: R20, Feb 2005.

2003

Fox CJ, Hammerman PS, Cinalli RM, Master SR, Chodosh LA, Thompson CB.: The serine/threonine kinase Pim-2 is a transcriptionally regulated apoptotic inhibitor. Genes Dev 17(15): 1841-54, Aug 2003.

2002

D'Cruz CM, Moody SE, Master SR, Hartman JL, Keiper EA, Imielinski MB, Cox JD, Wang JY, Ha SI, Keister BA, Chodosh LA.: Persistent parity-induced changes in growth factors, TGF-beta3, and differentiation in the rodent mammary gland. Mol Endocrinol 16(9): 2034-51, Sep 2002.

Li C, Fox CJ, Master SR, Bindokas VP, Chodosh LA, Thompson CB.: Bcl-X(L) affects Ca(2+) homeostasis by altering expression of inositol 1,4,5-trisphosphate receptors. Proc Natl Acad Sci U S A 99(15): 9830-5, July 2002.

Master SR, Hartman JL, D'Cruz CM, Moody SE, Keiper EA, Ha SI, Cox JD, Belka GK, Chodosh LA.: Functional microarray analysis of mammary organogenesis reveals a developmental role in adaptive thermogenesis. Mol Endocrinol 16(6): 1185-203, Jun 2002.

2001

Sarkisian CJ, Master SR, Huber LJ, Ha SI, Chodosh LA.: Analysis of murine Brca2 reveals conservation of protein-protein interactions but differences in nuclear localization signals. J Biol Chem 276(40): 37640-8, Oct 2001.

Huber LJ, Yang TW, Sarkisian CJ, Master SR, Deng CX, Chodosh LA.: Impaired DNA damage response in cells expressing an exon 11-deleted murine Brca1 variant that localizes to nuclear foci. Mol Cell Biol 21(12): 4005-15, Jun 2001.

1996

Poetter K, Jiang H, Hasssanzadeh S, Master SR, Chang A, Dalakas MC, Rayment I, Sellers JR, Fananapazir L, Epstein ND: Mutations in either the essential or regulatory light chains of myosin are associated with a rare myopathy in human heart and skeletal muscle. Nature Genetics 13(1): 63-9, May 1996.

Books

Chapters

2012

Master SR: Multiple Endocrine Neoplasia Type 2A. Clinical Decision Support: LABORATORY MEDICINE. Laposata MAlter DMelanson SEF (eds.). Decision Support in Medicine LLC, Wilmington, DE, 2012.

Master SR: Multiple Endocrine Neoplasia Type 1. Clinical Decision Support:LABORATORY MEDICINE. Laposata MAlter DMelanson SEF (eds.). Decision Support in Medicine LLC, Wilmington, DE, 2012.

Master SR: McCune-Albright Syndrome. Clinical Decision Support:LABORATORY MEDICINE. Laposata MAlter DMelanson SEF (eds.). Decision Support in Medicine LLC, Wilmington, DE, 2012.

Master SR: Multiple Endocrine Neoplasia Type 2b. Clinical Decision Support:LABORATORY MEDICINE. Laposata MAlter DMelanson SEF (eds.). Decision Support in Medicine LLC, Wilmington, DE, 2012.

Master SR: Polyglandular Autoimmune Syndrome. Clinical Decision Support:LABORATORY MEDICINE. Laposata MAlter DMelanson SEF (eds.). Decision Support in Medicine LLC, Wilmington, DE, 2012.

Kim AS, Master SR, Dunphy CH: Molecular Profiling Methods in the Diagnosis of Hematologic Disorders. Laboratory Hematology Practice. Kottke-Marchant K, Davis BH (eds.). Blackwell Publishing Limited, Page: 244, 2012.

2005

Kricka LJ, Master SR: Validation and Quality Control of Protein Microarray-Based Analytical Methods. Microarrays in Clinical Diagnostics. Joos TO, Fortina P (eds.). Humana Press, Page: 233-255, 2005.

Posters and Presentations

2017

Master SR: Problems with Non-Harmonized Results: How do we Prevent Them?   69th American Association for Clinical Chemistry Annual Scientific Meeting, San Diego, CA  Aug 2017 Notes: Oral Presentation.

Master SR: Using R to Build a Real Time Machine Learning Algorithm to Screen for Myelodysplastic Syndrome. 69th American Association for Clinical Chemistry Annual Scientific Meeting, San Diego, CA  Aug 2017 Notes: Oral Presentation.

Wertheim G, Pillai V, Sargent R, Paessler M, Lewen M, Li J, Master S, Carroll M, Luskin M: Immunohistochemistry Identifies AML Subtypes with Distinct Genetics and Prognosis. United States and Canadian Academy of Pathology 2017 Annual Meeting, San Antonio, Texas  Mar 2017 Notes: Poster Presentation.

Invited Lectures

2018

Master SR. "Using R for Method Validation Studies", AACC Annual Meeting, Chicago, IL. Jul, 2018.

Master SR. "Are you Feeding your Instruments the Right Water?", AACC Annual Meeting, Chicago, IL. Jul, 2018.

Master SR. "Bioinformatics and Quality Control for Multiplex Oncology Assays", Moffitt Cancer Center, Tampa, FL. May, 2018.

Master SR. "Bioinformatic Applications in Clinical Pathology", Pathology Grand Rounds, ARUP Laboratories (University of Utah), Salt Lake City, UT. Mar, 2018.

2017

Master SR. "FDA/CLIA Requirements for Validation of Laboratory Developed Tests in the USA", 29th World Congress of World Association of Societies of Pathology and Laboratory Medicine (WASPaLM), Kyoto, Japan. Nov, 2017.

Master SR. "Breaking Up with Excel: A Newbie's Guide to the R Statistical Programming Language" (2-day short course with Daniel Holmes), Mass Spectrometry Applications in the Clinical Lab EU 2017, Salzburg, Austria. Sep, 2017.

Master SR. "Communicating with Physicians: Case Studies from the US System", Chinese Society for Laboratory Medicine, Hongzhou, China. Sep, 2017.

Master SR. "Bioinformatic Applications in Clinical Pathology", Children's Hospital of Philadelphia, Philadelphia, PA. Jun, 2017.

Master SR. "Bioinformatic Applications in Clinical Pathology", Pathology Grand Rounds, Washington University St. Louis, MO. Mar, 2017.

Master SR. "Multiplex Clinical Quality Control for Emerging Diagnostics", Beth Israel Deaconess Hospital, Boston, MA. Mar, 2017.

Master SR. "Big Data in Medical Diagnostics", University of Pennsylvania Association of Senior and Emeritus Faculty, Philadelphia, PA. Feb, 2017.

Master SR. "Informatics Strategies for Multiplex Clinical Quality Control", CHOP Hematopathology Grand Rounds, Philadelphia, PA. Jan, 2017.

Master SR. "Breaking Up with Excel: A Newbie's Guide to the R Statistical Programming Language" (2-day short course with Daniel Holmes), Mass Spectrometry Applications in the Clinical Lab 2017, Palm Springs, CA. Jan, 2017.

Awards and Honors

2015, Outstanding Speaker Award, American Association for Clinical Chemistry
2015-2016, Weill Cornell Healthcare Leadership Fellowship Award, Weill Cornell Medical Center, New York Presbyterian
2012, Outstanding Speaker Award, American Association for Clinical Chemistry
2013, American Association for Clinical Chemistry, Division Achievement Award for "Advancing the Profession"
2004, William Pepper Fellowship Award in Laboratory Medicine, Hospital of the University of Pennsylvania
2003, Best Scientific Session, Microarray and Tissue Array Analysis
2001, Endocrine Society Travel Award
2001, Endocrine Society Medical Student Achievement Award, University of Pennsylvania School of Medicine
1996-1999, DOD Breast Cancer Predoctoral Training Award
1991, Dean's Letter of Commendation, SUNY at Buffalo School of Medicine
1990, Cum laude in Molecular Biology, Princeton University
1989-1990, NSF Summer Undergraduate Research Fellowship

Editorial and Academic Positions

Editorial Positions

2016-present, Clinical Mass Spectrometry, Editorial Board
2013-present, Cancer Research, Board of Editors
2013-present, Clinical Proteomics
 - 2016-present, Associate Editor
 - 2013-present, Editorial Board
2011-present, Clinical Chemistry
 - 2015-present, Associate Editor
 - 2011-present, Editorial Board
2010-present, BMC Cancer, reviewer
2010-present, American Journal of Obstetrics and Gynecology, reviewer
2008-present, Journal of Proteome Research, reviewer
2008-present, American Journal of Pathology, reviewer
2008-present, Archives of Pathology and Laboratory Medicine, section editor for clinical chemistry
2006-present, DNA and Cell Biology, reviewer
2006-present, Breast Cancer Research, reviewer
2005-present, Journal of Molecular Diagnostics, reviewer
2005-present, Clinical Chemistry, reviewer

Academic and Institutional Committees

2018-present, Institute for Biomedical Informatics, Perelman School of Medicine

Leadership and Memberships

Memberships in Professional Organizations

International

2018-present, Society for the Study of Inborn Errors of Metabolism
2013-present, International Consortium for Harmonization of Clinical Laboratory Results
 - 2013-present, Harmonization Oversight Group

National

2014-present, National Academy of Clinical Biochemistry
2006-present, Academy of Clinical Laboratory Physicians and Scientists
2005-present, College of American Pathologists
2005-present, American Society for Mass Spectrometry
2004-present, American Association for Clinical Chemistry
 - 2017-present, Board of Directors
 - 2017-present, Informatics Division Executive Committee Member
2000-present, American Association for the Advancement of Science