Melanie A. Ruffner, MD, PhD

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Melanie A. Ruffner, MD, PhD, is an attending physician with the Division of Allergy and Immunology and the Center for Pediatric Eosinophilic Disorders at Children's Hospital of Philadelphia.

Areas of Expertise: Food allergy, Eosinophilic esophagitis, Food protein induced enterocolitis syndrome, Care of patients with inherited deficiencies of the immune system, Environmental allergies, Atopic dermatitis, Asthma, Anaphylaxis
Locations: Buerger Center for Advanced Pediatric Care

Education and Training

Medical School

MD - University of Pittsburgh, School of Medicine, Pittsburgh, PA


Pediatrics - Children's Hospital of Philadelphia, Philadelphia, PA


Pediatrics - Children's Hospital of Philadelphia, Philadelphia, PA


Allergy & Immunology - Children's Hospital of Philadelphia, Philadelphia, PA
Post-Doctoral Fellow - Children's Hospital of Philadelphia Research Institute, Philadelphia, PA

Board Certification

Allergy and Immunology – American Board of Allergy and Immunology
Pediatrics – American Board of Pediatrics

Graduate Degree

PhD in Bioengineering - University of Pittsburgh, Pittsburgh, PA

Titles and Academic Titles

Attending Physician

Conditions Treated

Departments and Services




Godwin B, Liacouras C, Mehta V, Eisenberg J, Agawu A, Brown-Whitehorn TF, Ruffner MA Verma R, Cianferoni A, Spergel JM, Muir AB: A Review of Tertiary Referrals for Management of Pediatric Esophageal Eosinophilia Frontiers Pediatrics June 2018 Notes:

Ruffner Melanie A, USIDNET Body Weight Group, Sullivan Kathleen E: Complications associated with underweight primary immunodeficiency patients: prevalence and associations within the USIDNET Registry Journal of Clinical Immunology 38(3): 283-293, April 2018.

Fahey Lisa, Guzek Ryan, Ruffner Melanie, Sullivan Kathleen, Spergel Jonathan, Cianferoni Antonella: EMSY is Increased and Activates TSLP & CCL5 Expression in Eosinophilic Esophagitis. Pediatric Allergy and Immunology April 2018.

Cianferoni Antonella, Ruffner Melanie A, Guzek Ryan, Guan Shabao, Brown-Whitehorn Terri, Spergel Jonathan M: Activated Th2 cells and milk-specific Th2 cells are increased in milk-induced eosinophilic esophagitis. Annals of Allergy, Asthma & Immunology 120(2): 177-183.e2, February 2018. PMCID: PMC5875940

Crowley Terrence B, Ruffner Melanie A, McDonald-McGinn Donna M, Sullivan Kathleen E: Variable immune deficiency related to deletion size in chromosome 22q11.2 deletion syndrome. American Journal of Medical Genetics, Part A January 2018 Notes:


Ruffner MA, Aksamit TR, Thomashow B, Choate R, DiMango A, Turino GM, O'Donnell AE, Johnson MM, Olivier KN, Fennelly K, Daley CL, Winthrop KL, Metersky ML, Salathe MA, Knowles MR, Daniels MLA, Noone PG, Tino G, Griffith DE, Sullivan KE.: Frequency of untreated hypogammaglobulinemia in bronchiectasis. Ann Allergy Asthma Immunol May 2017.

Ruffner Melanie A, Brown-Whitehorn Terri F, Verma Ritu, Cianferoni Antonella, Gober Laura, Shuker Michele, Muir Amanda B, Liacouras Chris A, Spergel Jonathan M: Clinical tolerance in eosinophilic esophagitis. The Journal of Allergy and Clinical Immunology: In Practice 6(2): 661-3, March 2017.


Ruffner Melanie A, Ruymann Kathryn, Barni Simona, Cianferoni Antonella, Brown-Whitehorn Terri, Spergel Jonathan M: Food protein-induced enterocolitis syndrome: insights from review of a large referral population. The Journal of Allergy and Clinical Immunology. In practice 1(4): 343-9, Jul-Aug 2013.


Yang Chenjie, Ruffner Melanie A, Kim Seon-Hee, Robbins Paul D: Plasma-derived MHC class II+ exosomes from tumor-bearing mice suppress tumor antigen-specific immune responses. European journal of immunology 42(7): 1778-84, Jul 2012. PMCID: PMC3471134


Ruffner Melanie A, Finegold David N, MacGinnitie Andrew J: Infant with unusual food reactions. Acta paediatrica (Oslo, Norway : 1992) 100(10): 1289, 1394-5, Oct 2011.


Ruffner Melanie A, Robbins Paul D: Dendritic cells transduced to express interleukin 4 reduce diabetes onset in both normoglycemic and prediabetic nonobese diabetic mice. PloS one 5(7): e11848, Jul 2010. PMCID: PMC2912295


Ruffner Melanie A, Kim Seon Hee, Bianco Nicole R, Francisco Loise M, Sharpe Arlene H, Robbins Paul D: B7-1/2, but not PD-L1/2 molecules, are required on IL-10-treated tolerogenic DC and DC-derived exosomes for in vivo function. European journal of immunology 39(11): 3084-90, Nov 2009. PMCID: PMC3075967

Bianco Nicole R, Kim Seon Hee, Ruffner Melanie A, Robbins Paul D: Therapeutic effect of exosomes from indoleamine 2,3-dioxygenase-positive dendritic cells in collagen-induced arthritis and delayed-type hypersensitivity disease models. Arthritis and rheumatism 60(2): 380-9, Feb 2009. PMCID: PMC3491653


Kim T-K, Sharma B, Williams C G, Ruffner M A, Malik A, McFarland E G, Elisseeff J H: Experimental model for cartilage tissue engineering to regenerate the zonal organization of articular cartilage. Osteoarthritis and cartilage 11(9): 653-64, Sep 2003.



Ruffner MA, Crowley TB, McDonald-McGinn DM, Sullivan KE: Chromosome 22q11.2 Deletion Size Affects Peripheral Lymphocyte Subset Counts. Clinical Immunology Society Annual Meeting April 2018.

Ruffner MA, Garabedian EK, Fuleihan RL, Sullivan KE, Spergel JM: Eosinophilic Gastrointestinal Disease in Patients with Primary Immunodeficiency American Academy of Allergy, Asthma and Immunodeficiency Annual Meeting March 2018.


Ruffner Melanie A, Raman Swathi, Mauer Kelly, Muir Amanda, Spergel Jonathan M, Sullivan Kathleen E: Role of Toll-like receptor signaling in epithelial barrier dysfunction in eosinophilic esophagitis. American College of Allergy, Asthma and Immunology Annual Meeting, Boston, MA October 2017.

Ruffner Melanie A, Brown-Whitehorn Terri F, Verma Ritu, Cianferoni Antonella, Gober Laura, Shuker Michelle, Muir Amanda B, Liacouras Chris A, Spergel Jonathan M: Investigation of complete clinical tolerance in a pediatric eosinophilic esophagitis population. 4th CURED Research Conference, Cincinnati OH October 2017.


Wang, K, Ruffner, M, Brown-Whitehorn, T, Spergel, J: Anaphylaxis to intralipid after bone marrow transplant. Annals of Allergy Asthma & Immunology 117(5 S): S33, Nov 2016.




Ruffner MA and Brown-Whitehorn TF: "A 5-month old with vomiting, lethargy, and pallor" Pediatric Allergy and Clinical Immunology. Nima Rezaei (eds.). Springer, 2018.


Ruffner MA, Plevy SE, Cheung AT: "Applications of Nucleotide-based Therapy in the Treatment of Inflammatory Bowel Disease." Gene Therapy for Autoimmune and Inflammatory Diseases Y. Chernajovsky and P. Robbins (eds.). Basel, Switzerland: Birkhäuser Verlag AG, Page: 19-34, 2010.

Gaddy DF, Ruffner MA, and Robbins PD: "Autoimmune disorders" A Guide to Human Gene Therapy. R. Herzog and S. Zolotukhin (eds.). New Jersey, USA: World Scientific, Page: 295-308, 2010.


Elisseeff J, Ruffner MA, Kim TK, Williams CG. : "Cellular Photoencapsulation in Hydrogels" Culture of Cells for Tissue Engineering I. Freshney and G. Vunjak-Novakovic (eds.). New Jersey, USA: John Wiley & Sons, Page: 214-236, 2006.

Posters and Presentations


Lisa Fahey, Ryan Guzek, Melanie A Ruffner, Kathleen Sullivan Jonathan Spergel, Antonella Cianferoni: EMSY Gene Silencing in the Esophageal Epithelium Gastroenterology 152(5): S855, May 2017 Notes: Presented at Digestive Diseases Week Annual Meeting 2017.

Invited Lectures 


Ruffner M A. "Eosinophilic Esophagitis 101: The Allergy Perspective," EoE Patient Education Day, Children's Hospital of Philadelphia

Ruffner M A. "Chromosome 22q11.2 Deletion Size Affects Peripheral Lymphocyte Subset Counts" Clinical Immunology Society 2018 Annual Meeting


Ruffner M A. "Newborn Screening for Severe Combined Immunodeficiency," St. Christopher's Hospital, Immunology Department Teaching Rounds

Ruffner M A. "Pediatric Eosinophilic Esophagitis," Crozer Pediatric Residency Teaching Conference

Ruffner M A. "Outgrowing eosinophilic esophagitis: it is possible," American Academy of Allergy, Asthma and Immunology Annual Meeting

Ruffner M A. "The Eosinophilic Esophagitis Transcriptome is Altered with Therapy," Children's Hospital of Philadelphia "Postdoc Seminar Series - A Focus on Trainee Researchers"


Ruffner M A. "Factors regulating inflammation in eosinophilic esophagitis," Children's Hospital of Pittsburgh Allergy Department Research Seminar

Ruffner M A. "Worsening lung findings in a SCID patient," Clinical Immunology Summer School in Primary Immunodeficiency Diseases

Ruffner M A. "Body weight and infectious outcomes in patients with primary immunodeficiency," American Academy of Allergy, Asthma and Immunology Annual Meeting

Awards and Honors

2018, US Immunodeficiency Network Travel Grant

2017, Pennsylvania Allergy Educational Research Fund Travel Scholarship

2017, 4th CURED Research and Patient Education Conference Travel Award

2016-2017, American College of Allergy, Asthma and Immunology Young Faculty Award

2016, Clinical Immunology Society's (CIS) School in Primary Immunodeficiency Diseases Attendee

2015-2018, American Academy of Allergy Asthma and Immunology FIT Travel Scholarship

2015-2017, NIH Institutional T32 "Children's Hospital of Philadelphia Institutional Training in Pediatrics" Fellowship

2015, Pennsylvania Allergy Educational Research Fund Award

2014, CHOP CARES Community Action Grant Awardee for "Modeling Reading Behavior Through Video"

2014-2015, American College of Allergy, Asthma and Immunology FIT Travel Scholarship

2011, American Academy of Allergy Asthma and Immunology Chrysalis Project Award

2009, Graduate Class Marshall, Swanson School of Engineering

2008-2011, NIH Individual NRSA F30, "IL-4 Overexpressing Dendritic Cells and Exosomes for Treatment of Type I Diabetes"

2006, George M. Bevier Fellowship, University of Pittsburgh School of Engineering

2006-2008, NIH Institutional T32 Fellowship "University of Pittsburgh/Carnegie Mellon Biotechnology Training Program"

2003, Johns Hopkins University School of Engineering general and departmental graduation honors

2003, Merit Award Undergraduate Division IV, Program for the Advancement of Arc Welded Design, Engineering and Fabrication. Entry: "Device to measure bioimpedance in tissue: Towards early detection of preterm labor."

2001-2003, Johns Hopkins University Dean's List

Editorial and Academic Positions

Editorial Positions

2018-present, Basic and Clinical Immunology, reviewer
2018-present, Annals of Allergy, Asthma, and Immunology, ad hoc reviewer
2017-present, Clinical & Experimental Allergy, ad hoc reviewer

Academic and Institutional Committees

2017-present, Non-Admission Committee Interviewer, Pediatric Residency Program

Leadership and Memberships

Memberships in Professional Organizations


2014-present, Clinical Immunology Society


2014-present, American College of Allergy, Asthma, and Immunology
2011-present, American Academy of Pediatrics
2011-present, American Academy of Allergy, Asthma, and Immunology
2009-present, Biomedical Engineering Society